Construct: sgRNA BRDN0001148242
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTCTACCGACTCATCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARAF (369)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75661
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
47563325 |
+ |
ARAF |
NNNTCTACCGACTCATCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
82088481 |
- |
FASN |
NNNTCTCCCCACTCATCAAG |
NGG |
2 |
0.175 |
Tier I |
| 3 |
NC_000006.12 |
6 |
17956115 |
+ |
KIF13A |
NNNTCTAAGGACTCATCAAG |
NGG |
2 |
0.4024 |
Tier II |
| 4 |
NC_000008.11 |
8 |
61586818 |
- |
ASPH |
NNNTCTACCAACTCAGCAAG |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000009.12 |
9 |
127381346 |
+ |
GARNL3 |
NNNTCTACAGACTCATCAAG |
NTG |
2 |
0.0334 |
Tier II |
| 6 |
NC_000013.11 |
13 |
41520084 |
- |
LOC105370174 |
NNNTCTAAGGACTCATCAAG |
NGG |
2 |
0.4024 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
57099835 |
- |
Gria1 |
NNNTCTTCTGACTCATCAAG |
NGG |
2 |
0.3828 |
Tier II |
| 2 |
NC_000084.6 |
18 |
56818971 |
- |
Marchf3 |
NNNTCTACAGACTCATCAAG |
NGC |
2 |
0.019 |
Tier II |
| 3 |
NC_000081.6 |
15 |
98721670 |
+ |
Ccdc65 |
NNNACTACCGACTCATCAAG |
NTG |
2 |
0.0186 |
Tier II |
| 4 |
NC_000069.6 |
3 |
145632323 |
+ |
Gm40164 |
NNNTCGACCGACTCATCAAC |
NGG |
2 |
0.2449 |
Tier III |
Other clones with same target sequence:
(none)
