Construct: sgRNA BRDN0001148253

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGGGCAAGGATGTAGGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME6 (10201)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76221

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 48295221 + NME6 NNNGGGCAAGGATGTAGGCT NGG 0 1.0 Tier I
2 NC_000001.11 1 92614615 - EVI5 NNNGGACAAAGATGTAGGCT NGG 2 0.9333 Tier II
3 NC_000006.12 6 88961154 - RNGTT NNNGGACAAAGATGTAGGCT NGG 2 0.9333 Tier II
4 NC_000002.12 2 148377234 + MBD5 NNNGGAAAAGGATGTAGGCT NGG 2 0.75 Tier II
5 NC_000007.14 7 7721990 - UMAD1 NNNGGGAAAAGATGTAGGCT NGG 2 0.7 Tier II
6 NC_000017.11 17 66659436 + PRKCA NNNAGTCAAGGATGTAGGCT NGG 2 0.6 Tier II
7 NC_000014.9 14 105179421 - NUDT14 NNNGGACACGGATGTAGGCT NGG 2 0.5714 Tier II
8 NC_000004.12 4 21520059 + KCNIP4 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
9 NC_000004.12 4 37565953 - C4orf19 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
10 NC_000006.12 6 83010750 + UBE3D NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
11 NC_000006.12 6 159215781 + FNDC1 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
12 NC_000006.12 6 168005721 + KIF25 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
13 NC_000006.12 6 170342118 + FAM120B NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
14 NC_000007.14 7 4194654 + SDK1 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
15 NC_000008.11 8 3670279 - CSMD1 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
16 NC_000009.12 9 100079250 - ERP44 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
17 NC_000009.12 9 112376702 + PTBP3 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
18 NC_000011.10 11 7550866 - PPFIBP2 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
19 NC_000011.10 11 85157119 - DLG2 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
20 NC_000011.10 11 85163050 - DLG2 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
21 NC_000012.12 12 66340780 + HELB NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
22 NC_000015.10 15 73582861 - NPTN NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
23 NC_000023.11 X 55034984 + PAGE2B NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier II
24 NC_000003.12 3 174883094 + NAALADL2 NNNGGGGAAAGATGTAGGCT NGG 2 0.4392 Tier II
25 NC_000012.12 12 51707484 + SCN8A NNNGGGGAAAGATGTAGGCT NGG 2 0.4392 Tier II
26 NC_000017.11 17 50349650 + XYLT2 NNNGGGCATGGATGTAGGCA NGG 2 0.3375 Tier II
27 NC_000023.11 X 31179684 - DMD NNNTGGCAAGGATGTAGACT NGG 2 0.2517 Tier II
28 NC_000003.12 3 68141668 + TAFA1 NNNGTGAAAGGATGTAGGCT NGG 2 0.225 Tier II
29 NC_000010.11 10 119350111 + GRK5 NNNGGGTAAGGATGCAGGCT NGG 2 0.2216 Tier II
30 NC_000008.11 8 27375329 + PTK2B NNNGGGCCAGGATGTAGCCT NGG 2 0.2041 Tier II
31 NC_000010.11 10 102800448 + WBP1L NNNGGGCAAGGAGGTAGTCT NGG 2 0.1739 Tier II
32 NC_000017.11 17 67423449 + PITPNC1 NNNAGGCAAGGATGTGGGCT NGG 2 0.1731 Tier II
33 NC_000020.11 20 42793751 - PTPRT NNNGGGCATGGATGCAGGCT NGG 2 0.1636 Tier II
34 NC_000011.10 11 120222024 + OAF NNNGGGCAAGGAGGAAGGCT NGG 2 0.151 Tier II
35 NC_000003.12 3 69529100 + FRMD4B NNNGGGCAAAGATGTAGGGT NGG 2 0.1167 Tier II
36 NC_000020.11 20 44149279 - JPH2 NNNGGGCTAGGCTGTAGGCT NGG 2 0.1128 Tier II
37 NC_000003.12 3 13861154 - WNT7A NNNGGGCAAGAATGTAGGCC NGG 2 0.0909 Tier II
38 NC_000011.10 11 126218839 + FAM118B NNNGGACAAGGATGGAGGCT NGG 2 0.05 Tier II
39 NC_000011.10 11 131851295 + NTM NNNGGGCAAGGAAGGAGGCT NGG 2 0.0346 Tier II
40 NC_000006.12 6 3368265 + SLC22A23 NNNTGGCAAGGATGTAGGCC NGG 2 0.0331 Tier II
41 NC_000020.11 20 33789395 - ZNF341 NNNGGGCAAGGAGGTAGGGT NGG 2 0.0326 Tier II
42 NC_000011.10 11 104142846 + PDGFD NNNGGGCAATGATGTAGGCT NTG 2 0.0317 Tier II
43 NC_000016.10 16 57069747 - NLRC5 NNNGGGCAAGGATGTGGGGT NGG 2 0.024 Tier II
44 NC_000017.11 17 58276181 - MPO NNNGGGCATGGATGTAGGCT NTG 2 0.0234 Tier II
45 NC_000002.12 2 95034620 - MAL NNNGGGCCAGGATGGAGGCT NGG 2 0.0214 Tier II
46 NC_000002.12 2 10411846 + HPCAL1 NNNGGGCAAGGAGGTAGGCT NGA 2 0.0181 Tier II
47 NC_000012.12 12 103143012 + C12orf42 NNNGGGCAAGGTTGGAGGCT NGG 2 0.0167 Tier II
48 NC_000001.11 1 94161830 + LOC105378858 NNNGGACAAAGATGTAGGCT NGG 2 0.9333 Tier III
49 NC_000004.12 4 187381905 + LOC339975 NNNGGACAAAGATGTAGGCT NGG 2 0.9333 Tier III
50 NC_000013.11 13 101006906 + NALCN-AS1 NNNGGACAAAGATGTAGGCT NGG 2 0.9333 Tier III
51 NC_000023.11 X 21899789 + LOC124905259 NNNGGAGAAGGATGTAGGCT NGG 2 0.4706 Tier III
52 NC_000002.12 2 185429204 - LOC124907919 NNNGGGGAAAGATGTAGGCT NGG 2 0.4392 Tier III
53 NC_000001.11 1 147435624 + LINC00624 NNNGGGCAAGGCTGTAGGCA NGG 2 0.148 Tier III
54 NC_000009.12 9 106029557 + LOC107987108 NNNGGGCAAGGATCTAGGCT NAG 2 0.1111 Tier III
55 NC_000020.11 20 33789395 - ZNF341-AS1 NNNGGGCAAGGAGGTAGGGT NGG 2 0.0326 Tier III
56 NC_000002.12 2 10411846 + LOC124905972 NNNGGGCAAGGAGGTAGGCT NGA 2 0.0181 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 109841942 - Nme6 NNNGGGCAAGGATATAGGCT NGG 1 0.75 Tier I
2 NC_000083.6 17 34982663 + Lsm2 NNNGGGCAAGGATGTAGTCG NGG 2 0.1176 Tier I
3 NC_000080.6 14 23654998 + Kcnma1 NNNGGGCAAAGATGAAGGCT NGG 2 0.5404 Tier II
4 NC_000073.6 7 43825689 + Klk6 NNNGGGTAAGGATGTAGGCA NGG 2 0.457 Tier II
5 NC_000075.6 9 105687435 + Pik3r4 NNNGGGGAAGGATGTAGGCA NGG 2 0.2647 Tier II
6 NC_000068.7 2 31481254 + Ass1 NNNGGGCCAGGATGAAGGCT NGG 2 0.2481 Tier II
7 NC_000072.6 6 124694751 + Lpcat3 NNNGGGGAAGGATCTAGGCT NGG 2 0.2017 Tier II
8 NC_000072.6 6 99332151 + Foxp1 NNNGGGCAAGGATATAGGCT NAG 2 0.1944 Tier II
9 NC_000069.6 3 108831969 - Stxbp3 NNNGGGCTAGCATGTAGGCT NGG 2 0.1837 Tier II
10 NC_000075.6 9 24158614 - Npsr1 NNNGGGCAAGGAAGTAGGCT NAG 2 0.1795 Tier II
11 NC_000084.6 18 36770787 + Hars NNNGGGCAGGGATGTGGGCT NGG 2 0.1282 Tier II
12 NC_000070.6 4 128790695 - Zfp362 NNNGGGCAAGGATGCAGGAT NGG 2 0.1169 Tier II
13 NC_000080.6 14 35321437 - Grid1 NNNGGGCTAGGATTTAGGCT NGG 2 0.1143 Tier II
14 NC_000074.6 8 49139680 - Tenm3 NNNGGGCAAGTATGTATGCT NGG 2 0.0962 Tier II
15 NC_000068.7 2 174116864 - Npepl1 NNNGGGCAAGGCTGCAGGCT NGG 2 0.0718 Tier II
16 NC_000077.6 11 4481441 + Mtmr3 NNNGGGCAAGGATATAGGCC NGG 2 0.0682 Tier II
17 NC_000075.6 9 80366398 + Impg1 NNNGGGCAAGGTTGTAGGGT NGG 2 0.0417 Tier II
18 NC_000069.6 3 138212262 - Adh7 NNNGTGCAAGGATGTAGGGT NGG 2 0.0375 Tier II
19 NC_000072.6 6 48491387 - Sspo NNNGGGCAGGGATGGAGGCT NGG 2 0.0333 Tier II
20 NC_000073.6 7 143589819 - Cars NNNGGGCAAGGATGGAGCCT NGG 2 0.0238 Tier II
21 NC_000083.6 17 49514003 + Daam2 NNNGGGCAAGGCTGGAGGCT NGG 2 0.0132 Tier II
22 NC_000069.6 3 29124527 + Egfem1 NNNGGGCAAGGGTGTAGGCT NGT 2 0.0116 Tier II
23 NC_000070.6 4 128790695 - Gm52679 NNNGGGCAAGGATGCAGGAT NGG 2 0.1169 Tier III
24 NC_000068.7 2 164183506 - A730032A03Rik NNNGGGCAAGGAGCTAGGCT NGG 2 0.1118 Tier III
25 NC_000067.6 1 51627230 + Gm31636 NNNGGGCTAGGATGTGGGCT NGG 2 0.0824 Tier III
26 NC_000069.6 3 138212262 - 0610031O16Rik NNNGTGCAAGGATGTAGGGT NGG 2 0.0375 Tier III
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Other clones with same target sequence:

(none)