Construct: sgRNA BRDN0001148253
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGGCAAGGATGTAGGCT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NME6 (10201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 48295221 | + | NME6 | NNNGGGCAAGGATGTAGGCT | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 92614615 | - | EVI5 | NNNGGACAAAGATGTAGGCT | NGG | 2 | 0.9333 | Tier II |
3 | NC_000006.12 | 6 | 88961154 | - | RNGTT | NNNGGACAAAGATGTAGGCT | NGG | 2 | 0.9333 | Tier II |
4 | NC_000002.12 | 2 | 148377234 | + | MBD5 | NNNGGAAAAGGATGTAGGCT | NGG | 2 | 0.75 | Tier II |
5 | NC_000007.14 | 7 | 7721990 | - | UMAD1 | NNNGGGAAAAGATGTAGGCT | NGG | 2 | 0.7 | Tier II |
6 | NC_000017.11 | 17 | 66659436 | + | PRKCA | NNNAGTCAAGGATGTAGGCT | NGG | 2 | 0.6 | Tier II |
7 | NC_000014.9 | 14 | 105179421 | - | NUDT14 | NNNGGACACGGATGTAGGCT | NGG | 2 | 0.5714 | Tier II |
8 | NC_000004.12 | 4 | 21520059 | + | KCNIP4 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
9 | NC_000004.12 | 4 | 37565953 | - | C4orf19 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
10 | NC_000006.12 | 6 | 83010750 | + | UBE3D | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
11 | NC_000006.12 | 6 | 159215781 | + | FNDC1 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
12 | NC_000006.12 | 6 | 168005721 | + | KIF25 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
13 | NC_000006.12 | 6 | 170342118 | + | FAM120B | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
14 | NC_000007.14 | 7 | 4194654 | + | SDK1 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
15 | NC_000008.11 | 8 | 3670279 | - | CSMD1 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
16 | NC_000009.12 | 9 | 100079250 | - | ERP44 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
17 | NC_000009.12 | 9 | 112376702 | + | PTBP3 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
18 | NC_000011.10 | 11 | 7550866 | - | PPFIBP2 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
19 | NC_000011.10 | 11 | 85157119 | - | DLG2 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
20 | NC_000011.10 | 11 | 85163050 | - | DLG2 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
21 | NC_000012.12 | 12 | 66340780 | + | HELB | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
22 | NC_000015.10 | 15 | 73582861 | - | NPTN | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
23 | NC_000023.11 | X | 55034984 | + | PAGE2B | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier II |
24 | NC_000003.12 | 3 | 174883094 | + | NAALADL2 | NNNGGGGAAAGATGTAGGCT | NGG | 2 | 0.4392 | Tier II |
25 | NC_000012.12 | 12 | 51707484 | + | SCN8A | NNNGGGGAAAGATGTAGGCT | NGG | 2 | 0.4392 | Tier II |
26 | NC_000017.11 | 17 | 50349650 | + | XYLT2 | NNNGGGCATGGATGTAGGCA | NGG | 2 | 0.3375 | Tier II |
27 | NC_000023.11 | X | 31179684 | - | DMD | NNNTGGCAAGGATGTAGACT | NGG | 2 | 0.2517 | Tier II |
28 | NC_000003.12 | 3 | 68141668 | + | TAFA1 | NNNGTGAAAGGATGTAGGCT | NGG | 2 | 0.225 | Tier II |
29 | NC_000010.11 | 10 | 119350111 | + | GRK5 | NNNGGGTAAGGATGCAGGCT | NGG | 2 | 0.2216 | Tier II |
30 | NC_000008.11 | 8 | 27375329 | + | PTK2B | NNNGGGCCAGGATGTAGCCT | NGG | 2 | 0.2041 | Tier II |
31 | NC_000010.11 | 10 | 102800448 | + | WBP1L | NNNGGGCAAGGAGGTAGTCT | NGG | 2 | 0.1739 | Tier II |
32 | NC_000017.11 | 17 | 67423449 | + | PITPNC1 | NNNAGGCAAGGATGTGGGCT | NGG | 2 | 0.1731 | Tier II |
33 | NC_000020.11 | 20 | 42793751 | - | PTPRT | NNNGGGCATGGATGCAGGCT | NGG | 2 | 0.1636 | Tier II |
34 | NC_000011.10 | 11 | 120222024 | + | OAF | NNNGGGCAAGGAGGAAGGCT | NGG | 2 | 0.151 | Tier II |
35 | NC_000003.12 | 3 | 69529100 | + | FRMD4B | NNNGGGCAAAGATGTAGGGT | NGG | 2 | 0.1167 | Tier II |
36 | NC_000020.11 | 20 | 44149279 | - | JPH2 | NNNGGGCTAGGCTGTAGGCT | NGG | 2 | 0.1128 | Tier II |
37 | NC_000003.12 | 3 | 13861154 | - | WNT7A | NNNGGGCAAGAATGTAGGCC | NGG | 2 | 0.0909 | Tier II |
38 | NC_000011.10 | 11 | 126218839 | + | FAM118B | NNNGGACAAGGATGGAGGCT | NGG | 2 | 0.05 | Tier II |
39 | NC_000011.10 | 11 | 131851295 | + | NTM | NNNGGGCAAGGAAGGAGGCT | NGG | 2 | 0.0346 | Tier II |
40 | NC_000006.12 | 6 | 3368265 | + | SLC22A23 | NNNTGGCAAGGATGTAGGCC | NGG | 2 | 0.0331 | Tier II |
41 | NC_000020.11 | 20 | 33789395 | - | ZNF341 | NNNGGGCAAGGAGGTAGGGT | NGG | 2 | 0.0326 | Tier II |
42 | NC_000011.10 | 11 | 104142846 | + | PDGFD | NNNGGGCAATGATGTAGGCT | NTG | 2 | 0.0317 | Tier II |
43 | NC_000016.10 | 16 | 57069747 | - | NLRC5 | NNNGGGCAAGGATGTGGGGT | NGG | 2 | 0.024 | Tier II |
44 | NC_000017.11 | 17 | 58276181 | - | MPO | NNNGGGCATGGATGTAGGCT | NTG | 2 | 0.0234 | Tier II |
45 | NC_000002.12 | 2 | 95034620 | - | MAL | NNNGGGCCAGGATGGAGGCT | NGG | 2 | 0.0214 | Tier II |
46 | NC_000002.12 | 2 | 10411846 | + | HPCAL1 | NNNGGGCAAGGAGGTAGGCT | NGA | 2 | 0.0181 | Tier II |
47 | NC_000012.12 | 12 | 103143012 | + | C12orf42 | NNNGGGCAAGGTTGGAGGCT | NGG | 2 | 0.0167 | Tier II |
48 | NC_000001.11 | 1 | 94161830 | + | LOC105378858 | NNNGGACAAAGATGTAGGCT | NGG | 2 | 0.9333 | Tier III |
49 | NC_000004.12 | 4 | 187381905 | + | LOC339975 | NNNGGACAAAGATGTAGGCT | NGG | 2 | 0.9333 | Tier III |
50 | NC_000013.11 | 13 | 101006906 | + | NALCN-AS1 | NNNGGACAAAGATGTAGGCT | NGG | 2 | 0.9333 | Tier III |
51 | NC_000023.11 | X | 21899789 | + | LOC124905259 | NNNGGAGAAGGATGTAGGCT | NGG | 2 | 0.4706 | Tier III |
52 | NC_000002.12 | 2 | 185429204 | - | LOC124907919 | NNNGGGGAAAGATGTAGGCT | NGG | 2 | 0.4392 | Tier III |
53 | NC_000001.11 | 1 | 147435624 | + | LINC00624 | NNNGGGCAAGGCTGTAGGCA | NGG | 2 | 0.148 | Tier III |
54 | NC_000009.12 | 9 | 106029557 | + | LOC107987108 | NNNGGGCAAGGATCTAGGCT | NAG | 2 | 0.1111 | Tier III |
55 | NC_000020.11 | 20 | 33789395 | - | ZNF341-AS1 | NNNGGGCAAGGAGGTAGGGT | NGG | 2 | 0.0326 | Tier III |
56 | NC_000002.12 | 2 | 10411846 | + | LOC124905972 | NNNGGGCAAGGAGGTAGGCT | NGA | 2 | 0.0181 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000075.6 | 9 | 109841942 | - | Nme6 | NNNGGGCAAGGATATAGGCT | NGG | 1 | 0.75 | Tier I |
2 | NC_000083.6 | 17 | 34982663 | + | Lsm2 | NNNGGGCAAGGATGTAGTCG | NGG | 2 | 0.1176 | Tier I |
3 | NC_000080.6 | 14 | 23654998 | + | Kcnma1 | NNNGGGCAAAGATGAAGGCT | NGG | 2 | 0.5404 | Tier II |
4 | NC_000073.6 | 7 | 43825689 | + | Klk6 | NNNGGGTAAGGATGTAGGCA | NGG | 2 | 0.457 | Tier II |
5 | NC_000075.6 | 9 | 105687435 | + | Pik3r4 | NNNGGGGAAGGATGTAGGCA | NGG | 2 | 0.2647 | Tier II |
6 | NC_000068.7 | 2 | 31481254 | + | Ass1 | NNNGGGCCAGGATGAAGGCT | NGG | 2 | 0.2481 | Tier II |
7 | NC_000072.6 | 6 | 124694751 | + | Lpcat3 | NNNGGGGAAGGATCTAGGCT | NGG | 2 | 0.2017 | Tier II |
8 | NC_000072.6 | 6 | 99332151 | + | Foxp1 | NNNGGGCAAGGATATAGGCT | NAG | 2 | 0.1944 | Tier II |
9 | NC_000069.6 | 3 | 108831969 | - | Stxbp3 | NNNGGGCTAGCATGTAGGCT | NGG | 2 | 0.1837 | Tier II |
10 | NC_000075.6 | 9 | 24158614 | - | Npsr1 | NNNGGGCAAGGAAGTAGGCT | NAG | 2 | 0.1795 | Tier II |
11 | NC_000084.6 | 18 | 36770787 | + | Hars | NNNGGGCAGGGATGTGGGCT | NGG | 2 | 0.1282 | Tier II |
12 | NC_000070.6 | 4 | 128790695 | - | Zfp362 | NNNGGGCAAGGATGCAGGAT | NGG | 2 | 0.1169 | Tier II |
13 | NC_000080.6 | 14 | 35321437 | - | Grid1 | NNNGGGCTAGGATTTAGGCT | NGG | 2 | 0.1143 | Tier II |
14 | NC_000074.6 | 8 | 49139680 | - | Tenm3 | NNNGGGCAAGTATGTATGCT | NGG | 2 | 0.0962 | Tier II |
15 | NC_000068.7 | 2 | 174116864 | - | Npepl1 | NNNGGGCAAGGCTGCAGGCT | NGG | 2 | 0.0718 | Tier II |
16 | NC_000077.6 | 11 | 4481441 | + | Mtmr3 | NNNGGGCAAGGATATAGGCC | NGG | 2 | 0.0682 | Tier II |
17 | NC_000075.6 | 9 | 80366398 | + | Impg1 | NNNGGGCAAGGTTGTAGGGT | NGG | 2 | 0.0417 | Tier II |
18 | NC_000069.6 | 3 | 138212262 | - | Adh7 | NNNGTGCAAGGATGTAGGGT | NGG | 2 | 0.0375 | Tier II |
19 | NC_000072.6 | 6 | 48491387 | - | Sspo | NNNGGGCAGGGATGGAGGCT | NGG | 2 | 0.0333 | Tier II |
20 | NC_000073.6 | 7 | 143589819 | - | Cars | NNNGGGCAAGGATGGAGCCT | NGG | 2 | 0.0238 | Tier II |
21 | NC_000083.6 | 17 | 49514003 | + | Daam2 | NNNGGGCAAGGCTGGAGGCT | NGG | 2 | 0.0132 | Tier II |
22 | NC_000069.6 | 3 | 29124527 | + | Egfem1 | NNNGGGCAAGGGTGTAGGCT | NGT | 2 | 0.0116 | Tier II |
23 | NC_000070.6 | 4 | 128790695 | - | Gm52679 | NNNGGGCAAGGATGCAGGAT | NGG | 2 | 0.1169 | Tier III |
24 | NC_000068.7 | 2 | 164183506 | - | A730032A03Rik | NNNGGGCAAGGAGCTAGGCT | NGG | 2 | 0.1118 | Tier III |
25 | NC_000067.6 | 1 | 51627230 | + | Gm31636 | NNNGGGCTAGGATGTGGGCT | NGG | 2 | 0.0824 | Tier III |
26 | NC_000069.6 | 3 | 138212262 | - | 0610031O16Rik | NNNGTGCAAGGATGTAGGGT | NGG | 2 | 0.0375 | Tier III |