Construct: sgRNA BRDN0001148255
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGTTCAAAATAGCATAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PPIP5K2 (23262)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76341
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
103138433 |
- |
PPIP5K2 |
NNNTTCAAAATAGCATAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
69897290 |
+ |
CD226 |
NNNTTTAAAATAGCATAAAG |
NGG |
2 |
0.398 |
Tier II |
| 3 |
NC_000010.11 |
10 |
52043677 |
+ |
PRKG1 |
NNNTTCAAAATAGCAAAAAG |
NGG |
2 |
0.3896 |
Tier II |
| 4 |
NC_000010.11 |
10 |
15276991 |
- |
FAM171A1 |
NNNTTGAAAATAGCATAACT |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000001.11 |
1 |
75641638 |
+ |
SLC44A5 |
NNNTTCAAAATTGCATAACA |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000002.12 |
2 |
141452950 |
+ |
LRP1B |
NNNTTCAAAATAGCACAATG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000014.9 |
14 |
20366445 |
+ |
TEP1 |
NNNTTCAAAATACCATAACT |
NGG |
2 |
0.2947 |
Tier II |
| 8 |
NC_000007.14 |
7 |
11147446 |
- |
PHF14 |
NNNTTCAAAATAGCTTAAAG |
NGG |
2 |
0.0857 |
Tier II |
| 9 |
NC_000001.11 |
1 |
242307338 |
+ |
PLD5 |
NNNTTCAAAATAGCAAAACG |
NTG |
2 |
0.0354 |
Tier II |
| 10 |
NC_000006.12 |
6 |
6254359 |
+ |
F13A1 |
NNNTTCAAAATGGCATAACG |
NGC |
2 |
0.016 |
Tier II |
| 11 |
NC_000004.12 |
4 |
113124184 |
- |
ANK2 |
NNNTTCAAAACAGCATAACG |
NGT |
2 |
0.0108 |
Tier II |
| 12 |
NC_000003.12 |
3 |
78841182 |
+ |
ROBO1 |
NNNTTCAAAATAGGATAATG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000001.11 |
1 |
75641638 |
+ |
LOC100421536 |
NNNTTCAAAATTGCATAACA |
NGG |
2 |
0.3125 |
Tier III |
| 14 |
NC_000002.12 |
2 |
51956695 |
+ |
NRXN1-DT |
NNNTTCAAAATAGCACAAAG |
NGG |
2 |
0.2857 |
Tier III |
| 15 |
NC_000003.12 |
3 |
8598622 |
+ |
LOC107986009 |
NNNTTCAAAATAGCCTAACG |
NGG |
1 |
0.2727 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
74311828 |
+ |
Kdm4c |
NNNTTCAAAATGGCAAAACG |
NGG |
2 |
0.6566 |
Tier II |
| 2 |
NC_000072.6 |
6 |
82545620 |
+ |
Tacr1 |
NNNTTTAAAATAGCATAATG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000076.6 |
10 |
116928012 |
- |
Rab3ip |
NNNTTTAAAATAGCATAAAG |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000068.7 |
2 |
141533250 |
- |
Macrod2 |
NNNATCAAAATAGCATAATG |
NGG |
2 |
0.2198 |
Tier II |
| 5 |
NC_000085.6 |
19 |
6369946 |
- |
Sf1 |
NNNATCAAAATAGCATAAAG |
NGG |
2 |
0.2041 |
Tier II |
| 6 |
NC_000082.6 |
16 |
90879537 |
- |
Eva1c |
NNNTTCCAAATAGCATAGCG |
NGG |
2 |
0.175 |
Tier II |
| 7 |
NC_000072.6 |
6 |
27675151 |
+ |
Grm8 |
NNNTTCAAAATATCATAATG |
NGG |
2 |
0.1385 |
Tier II |
| 8 |
NC_000070.6 |
4 |
130681185 |
+ |
Pum1 |
NNNTTCAAAATAGCATAACA |
NGT |
2 |
0.0151 |
Tier II |
| 9 |
NC_000070.6 |
4 |
20507546 |
+ |
Nkain3 |
NNNTTCAAGATAGCATAACG |
NGT |
2 |
0.0108 |
Tier II |
| 10 |
NC_000076.6 |
10 |
116928012 |
- |
Gm51782 |
NNNTTTAAAATAGCATAAAG |
NGG |
2 |
0.398 |
Tier III |
Other clones with same target sequence:
(none)
