Construct: sgRNA BRDN0001148261

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTCACAATATTCAAACACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL1 (8814)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76270

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 50359082 + CDKL1 NNNCACAATATTCAAACACC NGG 0 1.0 Tier I
2 NC_000012.12 12 94247905 - PLXNC1 NNNCACAATATTCAGAGACC NGG 2 0.0382 Tier I
3 NC_000009.12 9 18395149 - ADAMTSL1 NNNCATAATACTCAAACACC NGG 2 0.619 Tier II
4 NC_000019.10 19 35763158 - PROSER3 NNNAACATTATTCAAACACC NGG 2 0.3609 Tier II
5 NC_000002.12 2 191110021 + STAT4 NNNCACAATATGCAAACTCC NGG 2 0.25 Tier II
6 NC_000023.11 X 134745776 - PLAC1 NNNCACAATATTCAAACAAA NGG 2 0.2143 Tier II
7 NC_000016.10 16 55696436 + SLC6A2 NNNCCCACTATTCAAACACC NGG 2 0.2143 Tier II
8 NC_000003.12 3 119996925 + GSK3B NNNCACAATGTTAAAACACC NGG 2 0.2137 Tier II
9 NC_000001.11 1 72072287 - NEGR1 NNNCACCATATTCAAACATC NGG 2 0.2019 Tier II
10 NC_000017.11 17 11296491 - SHISA6 NNNCACTATAGTCAAACACC NGG 2 0.175 Tier II
11 NC_000015.10 15 42391164 + CAPN3 NNNCACAATATTCAAACACA NAG 2 0.1296 Tier II
12 NC_000011.10 11 46540458 + AMBRA1 NNNCACAATATTCCAAAACC NGG 2 0.1 Tier II
13 NC_000013.11 13 97212244 - MBNL2 NNNCAGAATATTCAAACCCC NGG 2 0.0952 Tier II
14 NC_000006.12 6 591642 + EXOC2 NNNCACAATATTCTAACAGC NGG 2 0.0667 Tier II
15 NC_000003.12 3 325918 - CHL1 NNNCACACTATTCAAACAGC NGG 2 0.0536 Tier II
16 NC_000023.11 X 130724323 + ENOX2 NNNCAAAATATTCAAACACC NGC 2 0.0206 Tier II
17 NC_000014.9 14 33754800 - NPAS3 NNNCACAATAGTCAAACACC NTG 2 0.0156 Tier II
18 NC_000019.10 19 43555140 + XRCC1 NNNCACAATATTCAAACACC NTT 2 0.0 Tier II
19 NC_000001.11 1 210619432 - HHAT NNNCACAGTATTCAACCACC NGG 2 0.0 Tier II
20 NC_000005.10 5 61990346 - LOC124900610 NNNCGCAAAATTCAAACACC NGG 2 0.6686 Tier III
21 NC_000003.12 3 624924 - LINC01266 NNNCAAAATATTCAAAAACC NGG 2 0.4333 Tier III
22 NC_000014.9 14 25142421 - LINC02286 NNNTACAATATTAAAACACC NGG 2 0.3077 Tier III
23 NC_000013.11 13 64311304 - LOC124903237 NNNCACAATAGTCAAACACT NGG 2 0.12 Tier III
24 NC_000005.10 5 25281793 + LINC02211 NNNAACAATATTCAAAGACC NGG 2 0.0495 Tier III
25 NC_000009.12 9 82525260 - LOC107987087 NNNCACAATATCCAATCACC NGG 2 0.0 Tier III
26 NC_000006.12 6 96460251 + UFL1-AS1 NNNCACAATATTTAACCACC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 160865936 + Cdkl5 NNNCAACATATTCAAACACC NGG 2 0.4062 Tier I
2 NC_000081.6 15 7970006 + Wdr70 NNNCAAAGTATTCAAACACC NGG 2 0.681 Tier II
3 NC_000084.6 18 64579365 + Atp8b1 NNNCACAAGATTCAGACACC NGG 2 0.4024 Tier II
4 NC_000075.6 9 105271802 + Nek11 NNNTAGAATATTCAAACACC NGG 2 0.4 Tier II
5 NC_000071.6 5 52552820 + Lgi2 NNNCACACAATTCAAACACC NGG 2 0.398 Tier II
6 NC_000076.6 10 85465058 + Btbd11 NNNCACCATATTCAAATACC NGG 2 0.2042 Tier II
7 NC_000080.6 14 62588298 - Fam124a NNNCACAATTTTCAAACCCC NGG 2 0.1681 Tier II
8 NC_000076.6 10 28830066 - Themis NNNCACAATCTTCAAACACA NGG 2 0.1667 Tier II
9 NC_000081.6 15 26541742 + Fbxl7 NNNAACAATATTGAAACACC NGG 2 0.1148 Tier II
10 NC_000070.6 4 155130884 + Morn1 NNNCAGAATATTGAAACACC NGG 2 0.0682 Tier II
11 NC_000083.6 17 8963338 - Pde10a NNNCACAATATCCAAACACC NTG 2 0.0369 Tier II
12 NC_000067.6 1 41862980 + Gm29260 NNNCAAAATAATCAAACACC NGG 2 0.6964 Tier III
13 NC_000076.6 10 87020642 - Gm16271 NNNCATAATAGTCAAACACC NGG 2 0.3714 Tier III
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Other clones with same target sequence:

(none)