Construct: sgRNA BRDN0001148269
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACTACTATACAGACTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL1 (8814)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76268
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50341206 |
- |
CDKL1 |
NNNCTACTATACAGACTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
100647027 |
- |
ACTL6B |
NNNCTACAATACAGACTACG |
NCG |
2 |
0.0857 |
Tier I |
| 3 |
NC_000018.10 |
18 |
13242557 |
- |
LDLRAD4 |
NNNATACTATACAGACTACA |
NGG |
2 |
0.7895 |
Tier II |
| 4 |
NC_000012.12 |
12 |
103765345 |
+ |
NT5DC3 |
NNNCTACTATTCAGACTACA |
NGG |
2 |
0.2885 |
Tier II |
| 5 |
NC_000012.12 |
12 |
103765345 |
+ |
STAB2 |
NNNCTACTATTCAGACTACA |
NGG |
2 |
0.2885 |
Tier II |
| 6 |
NC_000006.12 |
6 |
5347489 |
- |
FARS2 |
NNNCTACTATACACACAACG |
NGG |
2 |
0.2286 |
Tier II |
| 7 |
NC_000009.12 |
9 |
18809961 |
- |
ADAMTSL1 |
NNNCTACTATACAGACTAAC |
NGG |
2 |
0.1837 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
69756620 |
- |
Cdkl1 |
NNNCTACTACACGGACTACG |
NGG |
2 |
0.3478 |
Tier I |
| 2 |
NC_000083.6 |
17 |
73920114 |
- |
Xdh |
NNNCTACCATACAGACTACA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000082.6 |
16 |
75383522 |
+ |
Robo2 |
NNNCTACTATGCAGACTACT |
NGG |
2 |
0.455 |
Tier II |
| 4 |
NC_000082.6 |
16 |
75383522 |
+ |
Gm30116 |
NNNCTACTATGCAGACTACT |
NGG |
2 |
0.455 |
Tier III |
Other clones with same target sequence:
(none)
