Construct: sgRNA BRDN0001148273

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGGAGGCTAAGGTGAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERK (64781)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76330

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 46712202 + CERK NNNGAGGCTAAGGTGAACAG NGG 0 1.0 Tier I
2 NC_000018.10 18 75005921 + ZNF407 NNNGAGACTAAAGTGAACAG NGG 2 0.9333 Tier II
3 NC_000023.11 X 56814815 - NBDY NNNGAGTCTAAGGTGAACAG NGG 1 0.5714 Tier II
4 NC_000007.14 7 101626620 + MYL10 NNNCAGGCAAAGGTGAACAG NGG 2 0.4916 Tier II
5 NC_000004.12 4 4748297 + LOC124900165 NNNAAGGCTAAGGTGAAAAG NGG 2 0.4846 Tier II
6 NC_000003.12 3 10689909 + ATP2B2 NNNGAGGGTAGGGTGAACAG NGG 2 0.4179 Tier II
7 NC_000011.10 11 74819444 - RNF169 NNNGAGGAGAAGGTGAACAG NGG 2 0.4024 Tier II
8 NC_000016.10 16 12373724 - SNX29 NNNGATGCTAAGGTGAAAAG NGG 2 0.359 Tier II
9 NC_000004.12 4 140495115 - MGAT4D NNNGAGGCCAATGTGAACAG NGG 2 0.355 Tier II
10 NC_000002.12 2 84833665 - TRABD2A NNNAAGGCTAAGTTGAACAG NGG 2 0.27 Tier II
11 NC_000005.10 5 119371800 + TNFAIP8 NNNGAGGCCAAGGTGGACAG NGG 2 0.1775 Tier II
12 NC_000010.11 10 26537432 - APBB1IP NNNGAGGCTAAGGAGAACAG NAG 2 0.1605 Tier II
13 NC_000007.14 7 5599140 - FSCN1 NNNGAGGATAAGGTGAGCAG NGG 2 0.1147 Tier II
14 NC_000008.11 8 42976280 + HOOK3 NNNGAGGCTGAGGTGGACAG NGG 2 0.1068 Tier II
15 NC_000020.11 20 19910417 - RIN2 NNNGAGGCCAAGGTGAACAG NCG 2 0.0989 Tier II
16 NC_000022.11 22 45934286 + WNT7B NNNGAGGCTAAGGTGACCTG NGG 2 0.095 Tier II
17 NC_000020.11 20 32743139 - COMMD7 NNNGAGGCTAAGGTGAGCTG NGG 2 0.095 Tier II
18 NC_000007.14 7 148013670 - CNTNAP2 NNNTAGGCTAAGGTGATCAG NGG 2 0.0485 Tier II
19 NC_000001.11 1 155728950 - DAP3 NNNGAGGCTAAGGTTAACAG NAG 2 0.037 Tier II
20 NC_000019.10 19 15622563 + CYP4F8 NNNGAGGCTAAGATGAACAG NGC 2 0.0205 Tier II
21 NC_000010.11 10 92341899 - MARCHF5 NNNGAGGCTAAGGTGGACAG NTG 2 0.0075 Tier II
22 NC_000019.10 19 12332173 + ZNF563 NNNAAGGCTAAGGGGAACAG NGG 2 0.0 Tier II
23 NC_000017.11 17 31484742 - RAB11FIP4 NNNGAGGCTGAGGGGAACAG NGG 2 0.0 Tier II
24 NC_000007.14 7 92807095 - CDK6 NNNGAGGTTAAGGGGAACAG NGG 2 0.0 Tier II
25 NC_000005.10 5 36243384 + LOC124900962 NNNGAGGCCAGGGTGAACAG NGG 2 0.6 Tier III
26 NC_000016.10 16 12373724 - LOC105371087 NNNGATGCTAAGGTGAAAAG NGG 2 0.359 Tier III
27 NC_000008.11 8 2809716 - LOC105377785 NNNGATGCTAATGTGAACAG NGG 2 0.2564 Tier III
28 NC_000005.10 5 116604454 + SEMA6A-AS2 NNNGGGGCTAAGGTGAACAG NAG 2 0.1867 Tier III
29 NC_000006.12 6 135603457 - AHI1-DT NNNGGGGCTAAGGTGACCAG NGG 2 0.1271 Tier III
30 NC_000012.12 12 82135821 - LOC105369873 NNNGAGGCTAAGGTCATCAG NGG 2 0.0364 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 138241990 - Hp1bp3 NNNGGGGCTAAGATGAACAG NGG 2 0.6646 Tier II
2 NC_000078.6 12 17281412 - Pdia6 NNNGAGGAAAAGGTGAACAG NGG 2 0.6036 Tier II
3 NC_000074.6 8 83907223 + Adgrl1 NNNGAAGCTGAGGTGAACAG NGG 2 0.5556 Tier II
4 NC_000070.6 4 57664673 - Pakap NNNGAGGCCAAGGTGAACTG NGG 2 0.497 Tier II
5 NC_000075.6 9 46014323 - Sik3 NNNTAGGCTAAGGTAAACAG NGG 2 0.3422 Tier II
6 NC_000071.6 5 114264433 + Foxn4 NNNGAGACTATGGTGAACAG NGG 2 0.3077 Tier II
7 NC_000072.6 6 54069664 - Chn2 NNNGAGGCAAAGGTGAACAG NAG 2 0.2407 Tier II
8 NC_000076.6 10 88173416 + Nup37 NNNGAGGCTTAGGTCAACAG NGG 2 0.2406 Tier II
9 NC_000083.6 17 25080131 + Ift140 NNNGAGTCTAAGCTGAACAG NGG 2 0.2406 Tier II
10 NC_000083.6 17 25080131 + Tmem204 NNNGAGTCTAAGCTGAACAG NGG 2 0.2406 Tier II
11 NC_000077.6 11 53436332 - Gdf9 NNNGAGGCTAAGGTAGACAG NGG 2 0.181 Tier II
12 NC_000068.7 2 110907138 + Ano3 NNNGAGTCTATGGTGAACAG NGG 2 0.1758 Tier II
13 NC_000082.6 16 72403211 - Robo1 NNNGTGGCTAAGGTGAACAC NGG 2 0.1558 Tier II
14 NC_000075.6 9 119491731 - Scn5a NNNGAGGCTCAGGTGAACAC NGG 2 0.1429 Tier II
15 NC_000077.6 11 80862571 + Spaca3 NNNGAGGCTAAGGTAATCAG NGG 2 0.1255 Tier II
16 NC_000071.6 5 76735055 + Cracd NNNGAGGCAAAGGTGAACAG NGA 2 0.0645 Tier II
17 NC_000081.6 15 12635851 - Pdzd2 NNNGAGGCTCAGGTGGACAG NGG 2 0.0641 Tier II
18 NC_000085.6 19 17175612 + Prune2 NNNGAGGCTAATGTGAAGAG NGG 2 0.0513 Tier II
19 NC_000080.6 14 79152548 + Vwa8 NNNGAGGCTAAGGTGAAGAG NAG 2 0.0346 Tier II
20 NC_000076.6 10 10710999 + Grm1 NNNGATGCTAAGGTGAACAG NTG 2 0.026 Tier II
21 NC_000080.6 14 65992013 + Gulo NNNGAGGCTAAGGTGAAGAG NCG 2 0.0143 Tier II
22 NC_000074.6 8 3473844 - Tex45 NNNGAGGCTAAGGTGAAGAG NGT 2 0.0022 Tier II
23 NC_000082.6 16 92418831 - Rcan1 NNNGAGGCTAAGGTGAAGAG NGT 2 0.0022 Tier II
24 NC_000078.6 12 118063694 - Dnah11 NNNGAGGCCAAGGGGAACAG NGG 2 0.0 Tier II
25 NC_000076.6 10 52062085 - Ros1 NNNGAGGCTCAGGTGTACAG NGG 2 0.0 Tier II
26 NC_000077.6 11 71257311 - Nlrp1c-ps NNNAAAGCTAAGGTGAACAG NGG 2 0.9 Tier III
27 NC_000080.6 14 104836282 + D130009I18Rik NNNGAGGTTAAGGTGGACAG NGG 2 0.1683 Tier III
28 NC_000081.6 15 18257469 + 4921515E04Rik NNNGAGGCTAAGGTGAACAG NCT 2 0.0 Tier III
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Other clones with same target sequence:

(none)