Construct: sgRNA BRDN0001148273
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGAGGCTAAGGTGAACAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CERK (64781)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000022.11 | 22 | 46712202 | + | CERK | NNNGAGGCTAAGGTGAACAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000018.10 | 18 | 75005921 | + | ZNF407 | NNNGAGACTAAAGTGAACAG | NGG | 2 | 0.9333 | Tier II |
3 | NC_000023.11 | X | 56814815 | - | NBDY | NNNGAGTCTAAGGTGAACAG | NGG | 1 | 0.5714 | Tier II |
4 | NC_000007.14 | 7 | 101626620 | + | MYL10 | NNNCAGGCAAAGGTGAACAG | NGG | 2 | 0.4916 | Tier II |
5 | NC_000004.12 | 4 | 4748297 | + | LOC124900165 | NNNAAGGCTAAGGTGAAAAG | NGG | 2 | 0.4846 | Tier II |
6 | NC_000003.12 | 3 | 10689909 | + | ATP2B2 | NNNGAGGGTAGGGTGAACAG | NGG | 2 | 0.4179 | Tier II |
7 | NC_000011.10 | 11 | 74819444 | - | RNF169 | NNNGAGGAGAAGGTGAACAG | NGG | 2 | 0.4024 | Tier II |
8 | NC_000016.10 | 16 | 12373724 | - | SNX29 | NNNGATGCTAAGGTGAAAAG | NGG | 2 | 0.359 | Tier II |
9 | NC_000004.12 | 4 | 140495115 | - | MGAT4D | NNNGAGGCCAATGTGAACAG | NGG | 2 | 0.355 | Tier II |
10 | NC_000002.12 | 2 | 84833665 | - | TRABD2A | NNNAAGGCTAAGTTGAACAG | NGG | 2 | 0.27 | Tier II |
11 | NC_000005.10 | 5 | 119371800 | + | TNFAIP8 | NNNGAGGCCAAGGTGGACAG | NGG | 2 | 0.1775 | Tier II |
12 | NC_000010.11 | 10 | 26537432 | - | APBB1IP | NNNGAGGCTAAGGAGAACAG | NAG | 2 | 0.1605 | Tier II |
13 | NC_000007.14 | 7 | 5599140 | - | FSCN1 | NNNGAGGATAAGGTGAGCAG | NGG | 2 | 0.1147 | Tier II |
14 | NC_000008.11 | 8 | 42976280 | + | HOOK3 | NNNGAGGCTGAGGTGGACAG | NGG | 2 | 0.1068 | Tier II |
15 | NC_000020.11 | 20 | 19910417 | - | RIN2 | NNNGAGGCCAAGGTGAACAG | NCG | 2 | 0.0989 | Tier II |
16 | NC_000022.11 | 22 | 45934286 | + | WNT7B | NNNGAGGCTAAGGTGACCTG | NGG | 2 | 0.095 | Tier II |
17 | NC_000020.11 | 20 | 32743139 | - | COMMD7 | NNNGAGGCTAAGGTGAGCTG | NGG | 2 | 0.095 | Tier II |
18 | NC_000007.14 | 7 | 148013670 | - | CNTNAP2 | NNNTAGGCTAAGGTGATCAG | NGG | 2 | 0.0485 | Tier II |
19 | NC_000001.11 | 1 | 155728950 | - | DAP3 | NNNGAGGCTAAGGTTAACAG | NAG | 2 | 0.037 | Tier II |
20 | NC_000019.10 | 19 | 15622563 | + | CYP4F8 | NNNGAGGCTAAGATGAACAG | NGC | 2 | 0.0205 | Tier II |
21 | NC_000010.11 | 10 | 92341899 | - | MARCHF5 | NNNGAGGCTAAGGTGGACAG | NTG | 2 | 0.0075 | Tier II |
22 | NC_000019.10 | 19 | 12332173 | + | ZNF563 | NNNAAGGCTAAGGGGAACAG | NGG | 2 | 0.0 | Tier II |
23 | NC_000017.11 | 17 | 31484742 | - | RAB11FIP4 | NNNGAGGCTGAGGGGAACAG | NGG | 2 | 0.0 | Tier II |
24 | NC_000007.14 | 7 | 92807095 | - | CDK6 | NNNGAGGTTAAGGGGAACAG | NGG | 2 | 0.0 | Tier II |
25 | NC_000005.10 | 5 | 36243384 | + | LOC124900962 | NNNGAGGCCAGGGTGAACAG | NGG | 2 | 0.6 | Tier III |
26 | NC_000016.10 | 16 | 12373724 | - | LOC105371087 | NNNGATGCTAAGGTGAAAAG | NGG | 2 | 0.359 | Tier III |
27 | NC_000008.11 | 8 | 2809716 | - | LOC105377785 | NNNGATGCTAATGTGAACAG | NGG | 2 | 0.2564 | Tier III |
28 | NC_000005.10 | 5 | 116604454 | + | SEMA6A-AS2 | NNNGGGGCTAAGGTGAACAG | NAG | 2 | 0.1867 | Tier III |
29 | NC_000006.12 | 6 | 135603457 | - | AHI1-DT | NNNGGGGCTAAGGTGACCAG | NGG | 2 | 0.1271 | Tier III |
30 | NC_000012.12 | 12 | 82135821 | - | LOC105369873 | NNNGAGGCTAAGGTCATCAG | NGG | 2 | 0.0364 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000070.6 | 4 | 138241990 | - | Hp1bp3 | NNNGGGGCTAAGATGAACAG | NGG | 2 | 0.6646 | Tier II |
2 | NC_000078.6 | 12 | 17281412 | - | Pdia6 | NNNGAGGAAAAGGTGAACAG | NGG | 2 | 0.6036 | Tier II |
3 | NC_000074.6 | 8 | 83907223 | + | Adgrl1 | NNNGAAGCTGAGGTGAACAG | NGG | 2 | 0.5556 | Tier II |
4 | NC_000070.6 | 4 | 57664673 | - | Pakap | NNNGAGGCCAAGGTGAACTG | NGG | 2 | 0.497 | Tier II |
5 | NC_000075.6 | 9 | 46014323 | - | Sik3 | NNNTAGGCTAAGGTAAACAG | NGG | 2 | 0.3422 | Tier II |
6 | NC_000071.6 | 5 | 114264433 | + | Foxn4 | NNNGAGACTATGGTGAACAG | NGG | 2 | 0.3077 | Tier II |
7 | NC_000072.6 | 6 | 54069664 | - | Chn2 | NNNGAGGCAAAGGTGAACAG | NAG | 2 | 0.2407 | Tier II |
8 | NC_000076.6 | 10 | 88173416 | + | Nup37 | NNNGAGGCTTAGGTCAACAG | NGG | 2 | 0.2406 | Tier II |
9 | NC_000083.6 | 17 | 25080131 | + | Ift140 | NNNGAGTCTAAGCTGAACAG | NGG | 2 | 0.2406 | Tier II |
10 | NC_000083.6 | 17 | 25080131 | + | Tmem204 | NNNGAGTCTAAGCTGAACAG | NGG | 2 | 0.2406 | Tier II |
11 | NC_000077.6 | 11 | 53436332 | - | Gdf9 | NNNGAGGCTAAGGTAGACAG | NGG | 2 | 0.181 | Tier II |
12 | NC_000068.7 | 2 | 110907138 | + | Ano3 | NNNGAGTCTATGGTGAACAG | NGG | 2 | 0.1758 | Tier II |
13 | NC_000082.6 | 16 | 72403211 | - | Robo1 | NNNGTGGCTAAGGTGAACAC | NGG | 2 | 0.1558 | Tier II |
14 | NC_000075.6 | 9 | 119491731 | - | Scn5a | NNNGAGGCTCAGGTGAACAC | NGG | 2 | 0.1429 | Tier II |
15 | NC_000077.6 | 11 | 80862571 | + | Spaca3 | NNNGAGGCTAAGGTAATCAG | NGG | 2 | 0.1255 | Tier II |
16 | NC_000071.6 | 5 | 76735055 | + | Cracd | NNNGAGGCAAAGGTGAACAG | NGA | 2 | 0.0645 | Tier II |
17 | NC_000081.6 | 15 | 12635851 | - | Pdzd2 | NNNGAGGCTCAGGTGGACAG | NGG | 2 | 0.0641 | Tier II |
18 | NC_000085.6 | 19 | 17175612 | + | Prune2 | NNNGAGGCTAATGTGAAGAG | NGG | 2 | 0.0513 | Tier II |
19 | NC_000080.6 | 14 | 79152548 | + | Vwa8 | NNNGAGGCTAAGGTGAAGAG | NAG | 2 | 0.0346 | Tier II |
20 | NC_000076.6 | 10 | 10710999 | + | Grm1 | NNNGATGCTAAGGTGAACAG | NTG | 2 | 0.026 | Tier II |
21 | NC_000080.6 | 14 | 65992013 | + | Gulo | NNNGAGGCTAAGGTGAAGAG | NCG | 2 | 0.0143 | Tier II |
22 | NC_000074.6 | 8 | 3473844 | - | Tex45 | NNNGAGGCTAAGGTGAAGAG | NGT | 2 | 0.0022 | Tier II |
23 | NC_000082.6 | 16 | 92418831 | - | Rcan1 | NNNGAGGCTAAGGTGAAGAG | NGT | 2 | 0.0022 | Tier II |
24 | NC_000078.6 | 12 | 118063694 | - | Dnah11 | NNNGAGGCCAAGGGGAACAG | NGG | 2 | 0.0 | Tier II |
25 | NC_000076.6 | 10 | 52062085 | - | Ros1 | NNNGAGGCTCAGGTGTACAG | NGG | 2 | 0.0 | Tier II |
26 | NC_000077.6 | 11 | 71257311 | - | Nlrp1c-ps | NNNAAAGCTAAGGTGAACAG | NGG | 2 | 0.9 | Tier III |
27 | NC_000080.6 | 14 | 104836282 | + | D130009I18Rik | NNNGAGGTTAAGGTGGACAG | NGG | 2 | 0.1683 | Tier III |
28 | NC_000081.6 | 15 | 18257469 | + | 4921515E04Rik | NNNGAGGCTAAGGTGAACAG | NCT | 2 | 0.0 | Tier III |