Construct: sgRNA BRDN0001148315
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGTCTTACTAGGTCCTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- Negative control (unspec'd)
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80251
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178542544 |
+ |
TTN |
NNNATCTTAATAGGTCCTGT |
NGG |
2 |
0.78 |
Tier I |
| 2 |
NC_000002.12 |
2 |
115618579 |
+ |
DPP10 |
NNNGTCTTACTAGGTACTGT |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000019.10 |
19 |
10500324 |
- |
KEAP1 |
NNNGTTTTACTAGGTACTGT |
NGG |
2 |
0.9286 |
Tier II |
| 4 |
NC_000009.12 |
9 |
96757105 |
- |
ZNF510 |
NNNATCTTACTAGTTCCTGT |
NGG |
2 |
0.24 |
Tier II |
| 5 |
NC_000014.9 |
14 |
51620720 |
- |
FRMD6 |
NNNGTCTTACCAGGTCCTGC |
NGG |
2 |
0.0606 |
Tier II |
| 6 |
NC_000002.12 |
2 |
178542544 |
+ |
TTN-AS1 |
NNNATCTTAATAGGTCCTGT |
NGG |
2 |
0.78 |
Tier III |
| 7 |
NC_000002.12 |
2 |
51452999 |
- |
NRXN1-DT |
NNNGTCTTTCTAAGTCCTGT |
NGG |
2 |
0.5538 |
Tier III |
| 8 |
NC_000020.11 |
20 |
4193362 |
+ |
LINC01433 |
NNNAACTTACTAGGTCCTGT |
NGG |
2 |
0.45 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
110110684 |
- |
Tle7 |
NNNGTCGAACTAGGTCCTGT |
NGG |
2 |
0.4706 |
Tier II |
| 2 |
NC_000072.6 |
6 |
90571564 |
- |
Aldh1l1 |
NNNGGCTTGCTAGGTCCTGT |
NGG |
2 |
0.4267 |
Tier II |
| 3 |
NC_000073.6 |
7 |
66798357 |
+ |
Cers3 |
NNNGTCTTACTAGGTCATCT |
NGG |
2 |
0.2092 |
Tier II |
| 4 |
NC_000079.6 |
13 |
68712251 |
- |
Adcy2 |
NNNGTCTTTCTAGGTCCTGT |
NAG |
2 |
0.1556 |
Tier II |
| 5 |
NC_000075.6 |
9 |
117487947 |
- |
Rbms3 |
NNNGTCTTACTAGGGCATGT |
NGG |
2 |
0.0233 |
Tier II |
Other clones with same target sequence:
(none)
