GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001148326

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: TGGTGCAAGGCATTATACAA

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CDK19 (23097)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 76505

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000006.12	6	110632042	-	CDK19	NNNTGCAAGGCATTATACAA	NGG	0	1.0	Tier I
2	NC_000005.10	5	149170645	+	ABLIM3	NNNTGCAGGGCATTATACAT	NGG	2	0.44	Tier II
3	NC_000002.12	2	219115552	+	NHEJ1	NNNTGCAAAGCATTACACAA	NGG	2	0.4286	Tier II
4	NC_000004.12	4	120866796	-	PRDM5	NNNGGGAAGGCATTATACAA	NGG	2	0.3125	Tier II
5	NC_000007.14	7	84021949	+	SEMA3A	NNNTGCAAGATATTATACAA	NGG	2	0.2872	Tier II
6	NC_000007.14	7	114394666	-	FOXP2	NNNTGCCAGGCATAATACAA	NGG	2	0.2708	Tier II
7	NC_000022.11	22	50124195	-	MOV10L1	NNNTGCAAGGAATTAGACAA	NGG	2	0.2596	Tier II
8	NC_000001.11	1	216548815	-	ESRRG	NNNTGCAAGGCAATATACAA	NAG	2	0.1795	Tier II
9	NC_000011.10	11	130114347	-	APLP2	NNNTGCAAGGCATTCTATAA	NGG	2	0.1753	Tier II
10	NC_000002.12	2	127094299	+	BIN1	NNNTGCAAAGCCTTATACAA	NGG	2	0.1692	Tier II
11	NC_000003.12	3	19166409	-	KCNH8	NNNTGCAAAGCATTATACAA	NAG	2	0.1667	Tier II
12	NC_000013.11	13	41254741	+	MTRF1	NNNGGCAAGGGATTATACAA	NGG	2	0.1563	Tier II
13	NC_000002.12	2	86547219	-	CHMP3	NNNTGCCAGGCATTATGCAA	NGG	2	0.0772	Tier II
14	NC_000002.12	2	86547219	-	RNF103-CHMP3	NNNTGCCAGGCATTATGCAA	NGG	2	0.0772	Tier II
15	NC_000008.11	8	132978650	+	TG	NNNTGCAAGGGATTATACAA	NAG	2	0.0648	Tier II
16	NC_000010.11	10	67379439	+	CTNNA3	NNNTGAAAGGCATTATACAA	NGA	2	0.0645	Tier II
17	NC_000012.12	12	16473692	-	MGST1	NNNTGCAAGTCATTATACAA	NGA	2	0.0564	Tier II
18	NC_000007.14	7	36349103	-	MATCAP2	NNNTGCAAGGCCTTATACCA	NGG	2	0.0544	Tier II
19	NC_000001.11	1	184502318	+	C1orf21	NNNTGCAAGGCCTTTTACAA	NGG	2	0.0526	Tier II
20	NC_000008.11	8	99419865	+	VPS13B	NNNTGCAAGGCATTATACTA	NTG	2	0.021	Tier II
21	NC_000005.10	5	137868140	+	MYOT	NNNTGGAAGGCATTATACAA	NTG	2	0.0195	Tier II
22	NC_000004.12	4	101344374	-	PPP3CA	NNNTGAAAGGCATTATACAA	NGT	2	0.015	Tier II
23	NC_000012.12	12	109351602	-	MYO1H	NNNTGCAAGGCATTATAGAA	NGT	2	0.0022	Tier II
24	NC_000013.11	13	80699058	-	LOC105370278	NNNTGCAATGCAATATACAA	NGG	2	0.3692	Tier III
25	NC_000011.10	11	10551198	-	IRAG1-AS1	NNNTGGAAGGGATTATACAA	NGG	2	0.125	Tier III
26	NC_000023.11	X	82777193	+	LOC105373288	NNNTTCAAGGTATTATACAA	NGG	2	0.0923	Tier III
27	NC_000017.11	17	70034657	+	LOC105371881	NNNTGCAAGGCATTATTCTA	NGG	2	0.0718	Tier III
28	NC_000017.11	17	70034657	+	LOC112267896	NNNTGCAAGGCATTATTCTA	NGG	2	0.0718	Tier III
29	NC_000005.10	5	137868140	+	PKD2L2-DT	NNNTGGAAGGCATTATACAA	NTG	2	0.0195	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000076.6	10	40466758	+	Cdk19	NNNTGCCAGGCATTACACAA	NGG	2	0.2917	Tier I
2	NC_000076.6	10	86219462	-	Syn3	NNNTGCAAGACATTATACAG	NGG	2	0.7137	Tier II
3	NC_000067.6	1	71315235	-	Abca12	NNNTGCAAGGCATTGCACAA	NGG	2	0.4333	Tier II
4	NC_000072.6	6	41670430	+	Trpv5	NNNTGCAGGGCATTATAAAA	NGG	2	0.3949	Tier II
5	NC_000081.6	15	41099862	-	Zfpm2	NNNTGCATGGCATTAAACAA	NGG	2	0.3896	Tier II
6	NC_000083.6	17	66581839	+	Themis3	NNNTGGAAGGCATTATAAAA	NGG	2	0.2692	Tier II
7	NC_000075.6	9	28084253	-	Opcml	NNNTGCAAGGCTTTATAAAA	NGG	2	0.1795	Tier II
8	NC_000068.7	2	163413797	+	Oser1	NNNTGCAAGGCATTTTATAA	NGG	2	0.1286	Tier II
9	NC_000071.6	5	135406595	+	Hip1	NNNTGCAATGCATTATACAA	NGA	2	0.037	Tier II
10	NC_000068.7	2	54899353	+	Galnt13	NNNTGCAAGGCATTATAGAC	NGG	2	0.0303	Tier II
11	NC_000084.6	18	42096079	-	Sh3rf2	NNNTGCCAGGCATTATACAA	NGT	2	0.0071	Tier II
12	NC_000077.6	11	44402233	+	Il12b	NNNTGCAAGGTATTATACAA	NGT	2	0.005	Tier II
13	NC_000073.6	7	69614091	-	Gm7627	NNNTGCAAGACAATATACAA	NGG	2	0.6462	Tier III
14	NC_000074.6	8	77661634	+	4933431K23Rik	NNNTGCAAGGTATTATACAA	NAG	2	0.0798	Tier III

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Other clones with same target sequence:

(none)