Construct: sgRNA BRDN0001148333
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCATAGATAAGAACCCCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACA (5566)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75518
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14097450 |
+ |
PRKACA |
NNNATAGATAAGAACCCCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
32483278 |
- |
NRG1 |
NNNATAGACTAGAACCCCCA |
NGG |
2 |
0.8145 |
Tier II |
| 3 |
NC_000003.12 |
3 |
116273241 |
+ |
LSAMP |
NNNCTGGATAAGAACCCCCA |
NGG |
2 |
0.2521 |
Tier II |
| 4 |
NC_000017.11 |
17 |
48029564 |
- |
COPZ2 |
NNNCAAGATAAGAACCCCCA |
NGG |
2 |
0.1765 |
Tier II |
| 5 |
NC_000010.11 |
10 |
112678039 |
- |
VTI1A |
NNNATAGATAAAAACCCGCA |
NGG |
2 |
0.1244 |
Tier II |
| 6 |
NC_000023.11 |
X |
32070361 |
- |
DMD |
NNNATAGATGAGAAACCCCA |
NGG |
2 |
0.1235 |
Tier II |
| 7 |
NC_000013.11 |
13 |
99221619 |
- |
UBAC2 |
NNNATAGAAAAGAAGCCCCA |
NGG |
2 |
0.0464 |
Tier II |
| 8 |
NC_000005.10 |
5 |
71647741 |
+ |
MCCC2 |
NNNATAGATAAGAACCCCCC |
NGA |
2 |
0.0158 |
Tier II |
| 9 |
NC_000007.14 |
7 |
760515 |
- |
DNAAF5 |
NNNATAGATAAGAACCACCA |
NGC |
2 |
0.0104 |
Tier II |
| 10 |
NC_000020.11 |
20 |
8027074 |
- |
TMX4-AS1 |
NNNATAGAAAAGAACACCCA |
NGG |
2 |
0.9286 |
Tier III |
| 11 |
NC_000003.12 |
3 |
18512368 |
+ |
SATB1-AS1 |
NNNATAGCTAAGAACACCCA |
NGG |
2 |
0.4286 |
Tier III |
| 12 |
NC_000023.11 |
X |
137557942 |
+ |
LINC02931 |
NNNATAGATAAGAACTTCCA |
NGG |
2 |
0.1436 |
Tier III |
| 13 |
NC_000021.9 |
21 |
6296122 |
- |
LOC102724701 |
NNNATAAATAAGAACCCCCA |
NGT |
2 |
0.0161 |
Tier III |
| 14 |
NC_000009.12 |
9 |
80432654 |
- |
LOC105376103 |
NNNATAGATAAGAACACCCA |
NGT |
2 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
18784351 |
+ |
Gm4922 |
NNNATACAAAAGAACCCCCA |
NGG |
2 |
0.6384 |
Tier I |
| 2 |
NC_000074.6 |
8 |
62004172 |
+ |
Ddx60 |
NNNATAAATAAGAACCCTCA |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000070.6 |
4 |
126029231 |
- |
Csf3r |
NNNAGTGATAAGAACCCCCA |
NGG |
2 |
0.4571 |
Tier II |
| 4 |
NC_000073.6 |
7 |
24923489 |
- |
Arhgef1 |
NNNGTACATAAGAACCCCCA |
NGG |
2 |
0.4297 |
Tier II |
| 5 |
NC_000074.6 |
8 |
83264119 |
+ |
Tbc1d9 |
NNNATAGATAAGAACCCACG |
NGG |
2 |
0.4118 |
Tier II |
| 6 |
NC_000071.6 |
5 |
118719356 |
- |
Med13l |
NNNATAGATGAGAACCCACA |
NGG |
2 |
0.2991 |
Tier II |
| 7 |
NC_000074.6 |
8 |
119185439 |
+ |
Cdh13 |
NNNAGAGATAAGAACCCCTA |
NGG |
2 |
0.2954 |
Tier II |
| 8 |
NC_000085.6 |
19 |
43159991 |
- |
Hpse2 |
NNNAAATATAAGAACCCCCA |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000067.6 |
1 |
134227948 |
- |
Adora1 |
NNNCTAGAGAAGAACCCCCA |
NGG |
2 |
0.2185 |
Tier II |
| 10 |
NC_000076.6 |
10 |
58514871 |
- |
Ccdc138 |
NNNATAGATCAGAACCTCCA |
NGG |
2 |
0.1556 |
Tier II |
| 11 |
NC_000077.6 |
11 |
53071560 |
+ |
Fstl4 |
NNNATAGACAAGAAGCCCCA |
NGG |
2 |
0.0462 |
Tier II |
| 12 |
NC_000078.6 |
12 |
100808341 |
+ |
Dglucy |
NNNATAGATAAGAAAGCCCA |
NGG |
2 |
0.0342 |
Tier II |
| 13 |
NC_000068.7 |
2 |
25424011 |
+ |
Fut7 |
NNNAAAGATAAGAATCCCCA |
NGG |
2 |
0.0333 |
Tier II |
| 14 |
NC_000080.6 |
14 |
17938248 |
+ |
Thrb |
NNNATAGATAAGAAACCCCA |
NTG |
2 |
0.0087 |
Tier II |
| 15 |
NC_000079.6 |
13 |
61693874 |
+ |
LOC115488202 |
NNNACAGAAAAGAACCCCCA |
NGG |
2 |
0.9286 |
Tier III |
| 16 |
NC_000076.6 |
10 |
18784351 |
+ |
Gm32926 |
NNNATACAAAAGAACCCCCA |
NGG |
2 |
0.6384 |
Tier III |
| 17 |
NC_000068.7 |
2 |
172330703 |
- |
Gm14273 |
NNNATAAATAAGACCCCCCA |
NGG |
2 |
0.2143 |
Tier III |
Other clones with same target sequence:
(none)
