Construct: sgRNA BRDN0001148338
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCAATGCCAAATCGATCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2C (79837)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77681
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57595192 |
- |
PIP4K2C |
NNNCAATGCCAAATCGATCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
60183658 |
+ |
FHIT |
NNNCAATGCCAAGTCAATCA |
NGG |
2 |
0.6522 |
Tier II |
| 3 |
NC_000010.11 |
10 |
17317887 |
- |
ST8SIA6 |
NNNCAATGACAAATAGATCA |
NGG |
2 |
0.1905 |
Tier II |
| 4 |
NC_000001.11 |
1 |
147766368 |
+ |
GJA5 |
NNNTAATGCCAAATTGATCA |
NGG |
2 |
0.0533 |
Tier II |
| 5 |
NC_000012.12 |
12 |
77052559 |
+ |
E2F7 |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier II |
| 6 |
NC_000015.10 |
15 |
27470943 |
- |
GABRG3 |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier II |
| 7 |
NC_000001.11 |
1 |
147766368 |
+ |
LOC102723321 |
NNNTAATGCCAAATTGATCA |
NGG |
2 |
0.0533 |
Tier III |
| 8 |
NC_000006.12 |
6 |
141941467 |
+ |
LOC105378031 |
NNNCAATGCCAAATTGATCA |
NGC |
2 |
0.0015 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
107904386 |
+ |
Cpt2 |
NNNCAATGCCAAAGCCATCA |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
115621397 |
- |
Gpc5 |
NNNCAATTCCAAATCAATCA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000071.6 |
5 |
21958819 |
- |
Reln |
NNNCAATGTCAAATAGATCA |
NGG |
2 |
0.1944 |
Tier II |
| 4 |
NC_000070.6 |
4 |
83765924 |
- |
Ccdc171 |
NNNCAATTCCAAATAGATCA |
NGG |
2 |
0.1389 |
Tier II |
| 5 |
NC_000081.6 |
15 |
92508282 |
+ |
Pdzrn4 |
NNNCAATTCCAAATAGATCA |
NGG |
2 |
0.1389 |
Tier II |
| 6 |
NC_000084.6 |
18 |
61143870 |
+ |
Hmgxb3 |
NNNCAATGCCACATCGATCA |
NAG |
2 |
0.0682 |
Tier II |
| 7 |
NC_000069.6 |
3 |
144199790 |
- |
Lmo4 |
NNNCAGTGCCAAATGGATCA |
NGG |
2 |
0.0357 |
Tier II |
| 8 |
NC_000074.6 |
8 |
99135350 |
- |
Cdh8 |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier II |
| 9 |
NC_000075.6 |
9 |
3408145 |
- |
Cwf19l2 |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier II |
| 10 |
NC_000075.6 |
9 |
18393883 |
+ |
Naalad2 |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier II |
| 11 |
NC_000070.6 |
4 |
120103016 |
- |
Hivep3 |
NNNCAATGCCAAATAGATCA |
NTG |
2 |
0.0087 |
Tier II |
| 12 |
NC_000076.6 |
10 |
13557903 |
- |
Adat2 |
NNNCAATGCCAAATGGATCA |
NCG |
2 |
0.0054 |
Tier II |
| 13 |
NC_000070.6 |
4 |
33081595 |
- |
Gabrr2 |
NNNCAGTGCCAAATCCATCA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000079.6 |
13 |
50139938 |
- |
4933433N18Rik |
NNNCAATGACAAATTGATCA |
NGG |
2 |
0.0571 |
Tier III |
| 15 |
NC_000079.6 |
13 |
50270837 |
- |
Gm31126 |
NNNCAATGACAAATTGATCA |
NGG |
2 |
0.0571 |
Tier III |
| 16 |
NC_000072.6 |
6 |
70235698 |
+ |
Igk |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier III |
| 17 |
NC_000073.6 |
7 |
61255525 |
+ |
A230006K03Rik |
NNNCAATTCCAAATGGATCA |
NGG |
2 |
0.0313 |
Tier III |
Other clones with same target sequence:
(none)
