GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001148368

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: AGCAGAACGGAATATTCTGG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: RPS6KB1 (6198)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 75614

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000017.11	17	59926493	+	RPS6KB1	NNNAGAACGGAATATTCTGG	NGG	0	1.0	Tier I
2	NC_000012.12	12	125077624	-	AACS	NNNAGAATGTAATATTCTGG	NGG	2	0.7109	Tier II
3	NC_000002.12	2	95178430	+	ZNF2	NNNAGAATGGAATATTCAGG	NGG	2	0.5833	Tier II
4	NC_000008.11	8	107436390	-	ANGPT1	NNNAGAACATAATATTCTGG	NGG	2	0.5223	Tier II
5	NC_000012.12	12	69609612	-	LRRC10	NNNAGAACTAAATATTCTGG	NGG	2	0.4978	Tier II
6	NC_000008.11	8	92898957	-	TRIQK	NNNAGAAAGGAAAATTCTGG	NGG	2	0.45	Tier II
7	NC_000001.11	1	53779921	-	NDC1	NNNAGAAAGGAATATTCTTG	NGG	2	0.4333	Tier II
8	NC_000005.10	5	133577243	+	FSTL4	NNNAGAACAGGATATTCTGG	NGG	2	0.4179	Tier II
9	NC_000019.10	19	51874761	+	ZNF577	NNNAGAAGGGAATATGCTGG	NGG	2	0.2225	Tier II
10	NC_000018.10	18	48678624	+	CTIF	NNNAGAACTGATTATTCTGG	NGG	2	0.1778	Tier II
11	NC_000008.11	8	86923861	+	CNBD1	NNNAGAACAGAATATTCTGG	NGA	2	0.0446	Tier II
12	NC_000006.12	6	133256814	-	EYA4	NNNAGAACTGAATATTCTGG	NGA	2	0.037	Tier II
13	NC_000003.12	3	33185279	+	SUSD5	NNNAGAACAGAATATTCTGG	NGT	2	0.0104	Tier II
14	NC_000017.11	17	62247096	-	LOC100996361	NNNAGAATGGAATATTCTGG	NGG	1	0.875	Tier III
15	NC_000012.12	12	125077624	-	LOC105370052	NNNAGAATGTAATATTCTGG	NGG	2	0.7109	Tier III
16	NC_000009.12	9	103260452	-	LINC01492	NNNAGAAAGGAATATTCTGA	NGG	2	0.6094	Tier III
17	NC_000002.12	2	130130487	+	MED15P9	NNNAGTAGGGAATATTCTGG	NGG	2	0.4592	Tier III
18	NC_000005.10	5	133577243	+	LOC124901066	NNNAGAACAGGATATTCTGG	NGG	2	0.4179	Tier III
19	NC_000007.14	7	72997770	-	STAG3L3	NNNTGAAAGGAATATTCTGG	NGG	2	0.4136	Tier III
20	NC_000007.14	7	74882447	-	STAG3L2	NNNTGAAAGGAATATTCTGG	NGG	2	0.4136	Tier III
21	NC_000007.14	7	74882447	-	LOC124901673	NNNTGAAAGGAATATTCTGG	NGG	2	0.4136	Tier III
22	NC_000019.10	19	31991458	-	LINC01837	NNNGGAACAGAATATTCTGG	NGG	2	0.4018	Tier III
23	NC_000022.11	22	34762951	+	LINC02885	NNNAGACCAGAATATTCTGG	NGG	2	0.2812	Tier III
24	NC_000022.11	22	34762951	+	LOC105373014	NNNAGACCAGAATATTCTGG	NGG	2	0.2812	Tier III
25	NC_000017.11	17	20716541	+	LOC100287072	NNNAGAATGGAATATTCTGG	NAG	2	0.2269	Tier III
26	NC_000018.10	18	48678624	+	LOC105372107	NNNAGAACTGATTATTCTGG	NGG	2	0.1778	Tier III
27	NC_000009.12	9	2202252	-	LOC107987043	NNNAGAACAGAATATTCTGG	NGT	2	0.0104	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000077.6	11	86519982	-	Rps6kb1	NNNAGAGCGGAACATTCTGG	NGG	2	0.5573	Tier I
2	NC_000074.6	8	128437816	-	Nrp1	NNNAGAATGGAGTATTCTGG	NGG	2	0.6319	Tier II
3	NC_000069.6	3	69414974	-	Ppm1l	NNNAGAGCAGAATATTCTGG	NGG	2	0.4538	Tier II
4	NC_000072.6	6	83248647	-	Slc4a5	NNNAGAAAGGAATATTCAGG	NGG	2	0.4333	Tier II
5	NC_000075.6	9	34975234	-	Kirrel3	NNNAGAACAGAATATCCTGG	NGG	2	0.4286	Tier II
6	NC_000078.6	12	44520117	+	Nrcam	NNNAGAACAGAATATTTTGG	NGG	2	0.3	Tier II
7	NC_000083.6	17	56875491	+	Acsbg3	NNNAGAAGGGAATGTTCTGG	NGG	2	0.3	Tier II
8	NC_000073.6	7	132772302	+	Fam53b	NNNAGCACAGAATATTCTGG	NGG	2	0.2922	Tier II
9	NC_000074.6	8	70163496	+	Mef2b	NNNAGCACGGAATGTTCTGG	NGG	2	0.2121	Tier II
10	NC_000074.6	8	70163496	+	Gm45929	NNNAGCACGGAATGTTCTGG	NGG	2	0.2121	Tier II
11	NC_000071.6	5	98351502	+	Cfap299	NNNAGAAGGGAATATTCTGG	NTG	2	0.025	Tier II
12	NC_000068.7	2	74302765	-	4930441J16Rik	NNNAGAACAGAAAATTCTGG	NGG	2	0.4451	Tier III
13	NC_000075.6	9	34975234	-	Kirrel3os	NNNAGAACAGAATATCCTGG	NGG	2	0.4286	Tier III
14	NC_000078.6	12	36264960	+	Gm40392	NNNAGAATGGAATAGTCTGG	NGG	2	0.0438	Tier III
15	NC_000069.6	3	58136295	+	Gm40056	NNNGGAACGGAATATTCTGG	NGA	2	0.0434	Tier III

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Other clones with same target sequence:

(none)