Construct: sgRNA BRDN0001148372

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGATGATAACTTAATTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ONE_INTERGENIC_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80258

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 130230699 - ZNF280C NNNATGATAACTAAATTTTT NGG 2 0.4615 Tier I
2 NC_000019.10 19 36515779 - ZNF260 NNNATGATAACCTAATTTAT NGG 2 0.6767 Tier II
3 NC_000007.14 7 94590335 - SGCE NNNATGGTAACCTAATTTGT NGG 2 0.6687 Tier II
4 NC_000007.14 7 94590335 - CASD1 NNNATGGTAACCTAATTTGT NGG 2 0.6687 Tier II
5 NC_000008.11 8 28501424 - FZD3 NNNATGATAACTTAGATTGT NGG 2 0.5909 Tier II
6 NC_000007.14 7 13927760 - ETV1 NNNATGAAAACTTAATCTGT NGG 2 0.5647 Tier II
7 NC_000011.10 11 22819868 + SVIP NNNATGAATACTTAATTTGT NGG 2 0.48 Tier II
8 NC_000008.11 8 60699394 - CHD7 NNNATGATCACATAATTTGT NGG 2 0.4571 Tier II
9 NC_000007.14 7 44684572 + OGDH NNNATATTAACTTAATTTGT NGG 2 0.4375 Tier II
10 NC_000001.11 1 216720191 + ESRRG NNNATGATTACTTAATTTTT NGG 2 0.4 Tier II
11 NC_000004.12 4 39619048 - SMIM14 NNNATCATCACTTAATTTGT NGG 2 0.3896 Tier II
12 NC_000009.12 9 69338932 + ENTREP1 NNNAAGATAAATTAATTTGT NGG 2 0.375 Tier II
13 NC_000002.12 2 175094672 - ATF2 NNNATGATAACTTTATTTTT NGG 2 0.3556 Tier II
14 NC_000006.12 6 135356821 - AHI1 NNNTTGATAACTTAATATGT NGG 2 0.3394 Tier II
15 NC_000001.11 1 178407954 - RASAL2 NNNATGATAACTAAAGTTGT NGG 2 0.2396 Tier II
16 NC_000023.11 X 86421592 - DACH2 NNNATAATAACTTCATTTGT NGG 2 0.2143 Tier II
17 NC_000005.10 5 26901417 - CDH9 NNNATGATGATTTAATTTGT NGG 2 0.2051 Tier II
18 NC_000020.11 20 46264198 - CDH22 NNNATGATAACTTACTTTAT NGG 2 0.1948 Tier II
19 NC_000010.11 10 33327289 + NRP1 NNNATGATAACTTATTTTGA NGG 2 0.1125 Tier II
20 NC_000002.12 2 148135014 + MBD5 NNNATGATAACTTACTTGGT NGG 2 0.0909 Tier II
21 NC_000023.11 X 102765684 - ARMCX5-GPRASP2 NNNATGAAAACTTAATTTGT NGA 2 0.0556 Tier II
22 NC_000003.12 3 7624601 - GRM7 NNNATGATAACTAAATTTGT NGA 2 0.0481 Tier II
23 NC_000004.12 4 31115171 - PCDH7 NNNATGACAACTTAATTTGT NTG 2 0.039 Tier II
24 NC_000008.11 8 80052924 + TPD52 NNNATGAAAACTTAATTTGT NTG 2 0.0312 Tier II
25 NC_000008.11 8 80052924 + TPD52-MRPS28 NNNATGAAAACTTAATTTGT NTG 2 0.0312 Tier II
26 NC_000003.12 3 56558580 + CCDC66 NNNATGATAACTTAATTTAT NGT 2 0.0115 Tier II
27 NC_000003.12 3 94010637 - ARL13B NNNATGATAACTTAATTAGT NGT 2 0.0108 Tier II
28 NC_000013.11 13 110426465 - COL4A2 NNNATGTTAACTTAATTTGT NGT 2 0.0071 Tier II
29 NC_000004.12 4 122354540 + BLTP1 NNNATGATAACTTATTTTGT NGT 2 0.0032 Tier II
30 NC_000002.12 2 206243888 + CMKLR2-AS NNNATAATAACTTAATTAGT NGG 2 0.6667 Tier III
31 NC_000023.11 X 27234561 + LOC105373150 NNNTTGATAAATTAATTTGT NGG 2 0.4773 Tier III
32 NC_000014.9 14 22197036 - TRA NNNTTGAGAACTTAATTTGT NGG 2 0.4667 Tier III
33 NC_000010.11 10 69990334 - LINC02636 NNNTTTATAACTTAATTTGT NGG 2 0.4242 Tier III
34 NC_000004.12 4 98674035 - LOC112267901 NNNATGATAACTTACATTGT NGG 2 0.2479 Tier III
35 NC_000023.11 X 129182911 + LOC124905213 NNNATGATAACTTAATTTTT NAG 2 0.1728 Tier III
36 NC_000001.11 1 106059491 - LOC105378885 NNNATGATAAGTTAATTTTT NGG 2 0.1667 Tier III
37 NC_000005.10 5 117571647 + LINC00992 NNNATGATAACTTACTTTGT NAG 2 0.0707 Tier III
38 NC_000003.12 3 164490907 + LOC105374191 NNNATGATAACTTAAATTGT NGA 2 0.0631 Tier III
39 NC_000007.14 7 136211826 + LOC105375523 NNNATGATAAATTAATTTGT NGA 2 0.0521 Tier III
40 NC_000009.12 9 119442075 + LOC105376250 NNNATGATAACTAAATTTGT NGA 2 0.0481 Tier III
41 NC_000010.11 10 123957820 + LOC107984128 NNNATGAAAACTTAATTTGT NTG 2 0.0312 Tier III
42 NC_000007.14 7 80312126 - LOC107986744 NNNATTATAACTTAATTTGT NTG 2 0.026 Tier III
43 NC_000005.10 5 90225824 - LINC01339 NNNATGATAACTAAATTTGT NGT 2 0.0112 Tier III
44 NC_000003.12 3 191430586 + PYDC2-AS1 NNNATGATAACTTAATTTTT NGT 2 0.0108 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 146235487 - Itpr2 NNNATTAGAACTTAATTTGT NGG 2 0.4889 Tier II
2 NC_000077.6 11 6356624 - Ogdh NNNATGATAACTTAGTTTAT NGG 2 0.4643 Tier II
3 NC_000073.6 7 144386227 - Shank2 NNNATGATCACTTAATTTTT NGG 2 0.381 Tier II
4 NC_000080.6 14 56366286 + Atp12a NNNATGATAACTTTACTTGT NGG 2 0.3556 Tier II
5 NC_000081.6 15 12729283 - Pdzd2 NNNATGACAACTTACTTTGT NGG 2 0.2727 Tier II
6 NC_000075.6 9 95090095 - Slc9a9 NNNATGGTAATTTAATTTGT NGG 2 0.2172 Tier II
7 NC_000079.6 13 109783314 - Pde4d NNNATGATAACTTACTCTGT NGG 2 0.1925 Tier II
8 NC_000067.6 1 46597408 + Dnah7c NNNATGATAACCTATTTTGT NGG 2 0.1895 Tier II
9 NC_000085.6 19 17036670 + Prune2 NNNATGATAACTTGATTTGT NAG 2 0.121 Tier II
10 NC_000077.6 11 22130027 - Ehbp1 NNNATGATAAATTAATGTGT NGG 2 0.0882 Tier II
11 NC_000080.6 14 23749780 - Kcnma1 NNNATGATAACTTATTTTGT NAG 2 0.0519 Tier II
12 NC_000067.6 1 119643639 - Epb41l5 NNNATAATAACTTAATTTGT NTG 2 0.039 Tier II
13 NC_000076.6 10 52861472 + Slc35f1 NNNATGATAACATAATTTGT NTG 2 0.0312 Tier II
14 NC_000082.6 16 33648539 - Slc12a8 NNNATGATAAATTAATTTGT NTG 2 0.0292 Tier II
15 NC_000074.6 8 15939929 + Csmd1 NNNATGATAACTTGATTTGT NGT 2 0.0075 Tier II
16 NC_000072.6 6 146235487 - Gm35596 NNNATTAGAACTTAATTTGT NGG 2 0.4889 Tier III
17 NC_000073.6 7 144386227 - Gm14372 NNNATGATCACTTAATTTTT NGG 2 0.381 Tier III
18 NC_000067.6 1 137953422 - Gm4258 NNNATGATAAATTAATTTCT NGG 2 0.3362 Tier III
19 NC_000075.6 9 77375934 - 5730403I07Rik NNNATGTTAACTTCATTTGT NGG 2 0.0938 Tier III
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Other clones with same target sequence:

(none)