Construct: sgRNA BRDN0001148374

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTGCAGAAACCATGGCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR2A (92)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77931

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 147918521 + ACVR2A NNNGCAGAAACCATGGCTAG NGG 0 1.0 Tier I
2 NC_000020.11 20 52166073 - ZFP64 NNNGCAGACACCATAGCTAG NGG 2 0.5378 Tier II
3 NC_000005.10 5 146935782 + PPP2R2B NNNGTAGAAAACATGGCTAG NGG 2 0.4773 Tier II
4 NC_000008.11 8 1185663 + DLGAP2 NNNGTAGAAACCAAGGCTAG NGG 2 0.3939 Tier II
5 NC_000022.11 22 19106379 + DGCR2 NNNGCAGGAACCATGGCCAG NGG 2 0.3143 Tier II
6 NC_000001.11 1 47828992 + TRABD2B NNNGCAGGAACCATGGCTGG NGG 2 0.275 Tier II
7 NC_000023.11 X 104921751 - IL1RAPL2 NNNGCAGAAACCATGGCTAT NAG 2 0.1815 Tier II
8 NC_000007.14 7 50761128 + GRB10 NNNGCAGAAACCAAGGCTAG NAG 2 0.1605 Tier II
9 NC_000002.12 2 164670792 + COBLL1 NNNGCAGAAACCAAGGCTAG NGA 2 0.043 Tier II
10 NC_000001.11 1 162225280 + NOS1AP NNNGCACAAACCATGGCTAG NTG 2 0.0268 Tier II
11 NC_000020.11 20 9088077 - PLCB4 NNNGCAGAAACCATGGCTTG NGC 2 0.012 Tier II
12 NC_000001.11 1 109912410 + CSF1 NNNGCAGAAGCCAGGGCTAG NGG 2 0.0 Tier II
13 NC_000001.11 1 193847413 + LOC124904475 NNNGCAGAAACCATGGCTAA NGG 1 0.9375 Tier III
14 NC_000012.12 12 52348351 - KRT89P NNNGCAGAAACAATGGCTAG NGG 1 0.7143 Tier III
15 NC_000023.11 X 52587246 - LOC107985659 NNNGCAGAAACCATGGTTAG NGG 1 0.4667 Tier III
16 NC_000014.9 14 97501375 - LINC02325 NNNGCAGAAACAATGGCTGG NGG 2 0.2679 Tier III
17 NC_000010.11 10 28476529 + LINC02652 NNNGCAGAAACCATGGCAGG NGG 2 0.25 Tier III
18 NC_000005.10 5 172489296 + LOC105377727 NNNACAGAAAGCATGGCTAG NGG 2 0.225 Tier III
19 NC_000008.11 8 129090757 - LOC124902023 NNNGCAGAAACATTGGCTAG NGG 2 0.2143 Tier III
20 NC_000001.11 1 87244752 - LINC02801 NNNGCAGAAACCATGGCTCA NGG 2 0.194 Tier III
21 NC_000001.11 1 227319269 - LOC124904531 NNNGCAGTAACCATGGCTAG NGA 2 0.0298 Tier III
22 NC_000008.11 8 127851770 - PVT1 NNNGGAGAAACCATGGCTAG NGT 2 0.0097 Tier III
23 NC_000007.14 7 140961755 + LOC105375536 NNNGCAGAAACCATGTCTAG NTG 2 0.0 Tier III
24 NC_000011.10 11 87660519 - LOC107984361 NNNGCAGAAACCCGGGCTAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 48893581 + Acvr2a NNNGCAGAAACCATGGCTAG NGG 0 1.0 Tier I
2 NC_000067.6 1 171419131 + Tstd1 NNNGCAGGAACCATGGCTGG NGG 2 0.275 Tier I
3 NC_000085.6 19 20956570 + Tmc1 NNNGCAGGAACCATGACTAG NGG 2 0.7333 Tier II
4 NC_000067.6 1 43768198 - Uxs1 NNNACTGAAACCATGGCTAG NGG 2 0.6429 Tier II
5 NC_000080.6 14 34845658 - Grid1 NNNGCAGACACCATGGCTAG NGG 1 0.5714 Tier II
6 NC_000068.7 2 90562144 + Ptprj NNNGCAGAAACCATAGCTAC NGG 2 0.4034 Tier II
7 NC_000067.6 1 58255894 + Aox4 NNNGCAGAAACCATGGATAT NGG 2 0.3267 Tier II
8 NC_000078.6 12 76706303 - Sptb NNNGCAGAAAACATGGCCAG NGG 2 0.3214 Tier II
9 NC_000081.6 15 10693735 + Rai14 NNNCCAGAAGCCATGGCTAG NGG 2 0.2941 Tier II
10 NC_000071.6 5 31315909 + Gckr NNNGCATTAACCATGGCTAG NGG 2 0.2449 Tier II
11 NC_000071.6 5 114259913 - Foxn4 NNNGCAGAACCCATGGCAAG NGG 2 0.2222 Tier II
12 NC_000071.6 5 137421971 + Zan NNNGCAGAAACCAAGGCGAG NGG 2 0.2063 Tier II
13 NC_000073.6 7 91169429 - Dlg2 NNNACAGAAACCCTGGCTAG NGG 2 0.1895 Tier II
14 NC_000068.7 2 14209029 - Tmem236 NNNGCAGAAACCATGGATGG NGG 2 0.175 Tier II
15 NC_000070.6 4 131797488 + Ptpru NNNGCAGATACCCTGGCTAG NGG 2 0.1263 Tier II
16 NC_000072.6 6 6011919 - Dync1i1 NNNGCAGAAAACATGGCTAG NGA 2 0.0521 Tier II
17 NC_000067.6 1 115728033 + Cntnap5a NNNGCATAAACCATGGGTAG NGG 2 0.0336 Tier II
18 NC_000067.6 1 189359973 - Kcnk2 NNNGCAGAATCCATGGCTAG NGC 2 0.0196 Tier II
19 NC_000073.6 7 96903719 + Tenm4 NNNGCAGAAACCAAGCCTAG NGG 2 0.0 Tier II
20 NC_000070.6 4 118588702 - Cfap57 NNNGCAGAAACCAGGGCTAG NGA 2 0.0 Tier II
21 NC_000076.6 10 106699078 - Ppfia2 NNNGCAGAAACCATGGCTAG NTT 2 0.0 Tier II
22 NC_000068.7 2 127338087 + Dusp2 NNNGCAGAAACCATGTCTAG NGG 1 0.0 Tier II
23 NC_000073.6 7 144747218 + Ano1 NNNGCAGAGACCAGGGCTAG NGG 2 0.0 Tier II
24 NC_000067.6 1 90344392 + Gm34992 NNNGCAGAAGCCATGGCTAA NGG 2 0.5208 Tier III
25 NC_000080.6 14 71268657 + Gm4251 NNNGAAGGAACCATGGCTAG NGG 2 0.419 Tier III
26 NC_000077.6 11 109638637 - Gm11685 NNNGCAGAAACCATGGATAT NGG 2 0.3267 Tier III
27 NC_000072.6 6 115132968 - Gm17733 NNNGCTGAAATCATGGCTAG NGG 2 0.2198 Tier III
28 NC_000077.6 11 98300314 + Gm31317 NNNACAGAAACCCTGGCTAG NGG 2 0.1895 Tier III
29 NC_000075.6 9 47241436 - Gm39332 NNNTCAGAAATCATGGCTAG NGG 2 0.1119 Tier III
30 NC_000073.6 7 74849739 - 0610006L08Rik NNNGCAGAAAGCATGGCTAC NGG 2 0.1071 Tier III
31 NC_000072.6 6 135556776 - Gm25136 NNNGCAGAAAGCATGGCTGG NGG 2 0.0938 Tier III
32 NC_000069.6 3 144164853 + Gm34162 NNNGCAGAAACCATGGGTAG NGA 2 0.0041 Tier III
33 NC_000073.6 7 144747218 + D930030F02Rik NNNGCAGAGACCAGGGCTAG NGG 2 0.0 Tier III
34 NC_000081.6 15 90918308 - Gm36618 NNNGTAGAAACCATGCCTAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)