Construct: sgRNA BRDN0001148390
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACACCACTACACCATCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SYK (6850)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76776
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
90844097 |
+ |
SYK |
NNNACCACTACACCATCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
12950448 |
- |
PHACTR1 |
NNNACCACTCCACCATCAAG |
NGG |
2 |
0.2308 |
Tier II |
| 3 |
NC_000013.11 |
13 |
110939639 |
- |
PARP1P1 |
NNNACCTCTACACCATCCAG |
NGG |
2 |
0.2083 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
52611034 |
+ |
Syk |
NNNACCACTACACCATCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
134252137 |
- |
Grrp1 |
NNNCCCACTACACCTTCGAG |
NGG |
2 |
0.0706 |
Tier I |
| 3 |
NC_000071.6 |
5 |
86094788 |
+ |
Stap1 |
NNNACAACTACACCATCGAG |
NTG |
2 |
0.0362 |
Tier I |
| 4 |
NC_000071.6 |
5 |
73229058 |
+ |
Fryl |
NNNACCACTACACCATTCAG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000074.6 |
8 |
66442976 |
+ |
Marchf1 |
NNNACCACTACACCCTCTAG |
NGG |
2 |
0.1818 |
Tier II |
| 6 |
NC_000072.6 |
6 |
118048262 |
- |
Rasgef1a |
NNNACCACTGCACCATCGAG |
NAG |
2 |
0.144 |
Tier II |
| 7 |
NC_000072.6 |
6 |
147100612 |
- |
Klhl42 |
NNNACCACTACACCTTCCAG |
NGG |
2 |
0.0952 |
Tier II |
| 8 |
NC_000085.6 |
19 |
22450580 |
+ |
Trpm3 |
NNNACCACTACACCATCCAG |
NTG |
2 |
0.0186 |
Tier II |
Other clones with same target sequence:
(none)
