Construct: sgRNA BRDN0001148399

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGGTGCCATCCCTAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKD1 (5310)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76842

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 2110201 + PKD1 NNNGGTGCCATCCCTAACCA NGG 0 1.0 Tier I
2 NC_000019.10 19 30296605 + ZNF536 NNNGCTGCCATCTCTAACCA NGG 2 0.55 Tier II
3 NC_000012.12 12 52612568 + KRT73 NNNGGTGCCAACCCTAAACA NGG 2 0.4038 Tier II
4 NC_000007.14 7 44636438 - OGDH NNNGGTGCCACTCCTAACCA NGG 2 0.359 Tier II
5 NC_000017.11 17 35785023 - MMP28 NNNGGTCCCAGCCCTAACCA NGG 2 0.275 Tier II
6 NC_000010.11 10 16901048 - CUBN NNNGTTGCCATCCCAAACCA NGG 2 0.1737 Tier II
7 NC_000016.10 16 1414431 + UNKL NNNGGTGCCATCCCAGACCA NGG 2 0.1113 Tier II
8 NC_000010.11 10 33179414 - NRP1 NNNGGTGCCATCCCTGAACA NGG 2 0.1036 Tier II
9 NC_000009.12 9 72237551 + GDA NNNGGTGCCATCCACAACCA NGG 2 0.0955 Tier II
10 NC_000015.10 15 22918892 - CYFIP1 NNNGGTGCCATCCCTAAGAA NGG 2 0.0571 Tier II
11 NC_000010.11 10 25417808 - GPR158 NNNGGTGCCATCCCTAACCA NGT 1 0.0161 Tier II
12 NC_000017.11 17 18242416 + LLGL1 NNNGGTGCCACCCCTCACCA NGG 2 0.0 Tier II
13 NC_000006.12 6 25994498 - LINC02980 NNNGCTGCCATCCCTAACCA NGG 1 0.7857 Tier III
14 NC_000012.12 12 52612568 + KRT73-AS1 NNNGGTGCCAACCCTAAACA NGG 2 0.4038 Tier III
15 NC_000016.10 16 16365424 - PKD1P2 NNNGGTGCCATCCCTGACCA NGG 1 0.1923 Tier III
16 NC_000016.10 16 18386758 + PKD1P5 NNNGGTGCCGTCCCTGACCA NGG 2 0.1068 Tier III
17 NC_000016.10 16 18386758 + PKD1P5-LOC105376752 NNNGGTGCCGTCCCTGACCA NGG 2 0.1068 Tier III
18 NC_000004.12 4 38428492 + LINC01258 NNNGGTGCCATCACTTACCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 51451424 + Col23a1 NNNAGTACCATCCCTAACCA NGG 2 0.9 Tier II
2 NC_000080.6 14 67421363 + Ebf2 NNNGGTCTCATCCCTAACCA NGG 2 0.6016 Tier II
3 NC_000083.6 17 30127737 + Zfand3 NNNGGTGCCACCTCTAACCA NGG 2 0.4667 Tier II
4 NC_000072.6 6 32397472 - Plxna4 NNNGGTGCCATCCCAAACCT NGG 2 0.3474 Tier II
5 NC_000085.6 19 12706398 + Keg1 NNNGGTCCCATCACTAACCA NGG 2 0.2644 Tier II
6 NC_000078.6 12 99224149 - Foxn3 NNNGGTGCCATCCCTAGCCA NGG 1 0.1765 Tier II
7 NC_000070.6 4 152146293 - Espn NNNGGTGCCATCCCTACCCG NGG 2 0.1349 Tier II
8 NC_000071.6 5 30829908 - Mapre3 NNNGGGGCCATCCCTAAGCA NGG 2 0.0762 Tier II
9 NC_000067.6 1 156471848 + Soat1 NNNTGTGCCATCCCTACCCA NGG 2 0.0642 Tier II
10 NC_000074.6 8 87572060 + 4933402J07Rik NNNGGTCCCATCCCTAACCA NGA 2 0.0477 Tier II
11 NC_000074.6 8 118767909 - Cdh13 NNNGGTGCCATCCCTAGGCA NGG 2 0.0235 Tier II
12 NC_000076.6 10 90461668 - Anks1b NNNGGTGCCATCCCTAGGCA NGG 2 0.0235 Tier II
13 NC_000082.6 16 58440816 + Dcbld2 NNNGGTGCCATGCCTAACCA NTG 2 0.0173 Tier II
14 NC_000073.6 7 62807137 - A26c2 NNNGGTGCCATCCCTACCCA NTG 2 0.0069 Tier II
15 NC_000072.6 6 80785056 + Lrrtm4 NNNGGTGCCATCCCTGACCA NGT 2 0.0031 Tier II
16 NC_000071.6 5 38293271 - Otop1 NNNGGTGCCATCCCTAAGCA NGT 2 0.0022 Tier II
17 NC_000082.6 16 73380923 - 4930500H12Rik NNNGGTCCCATCCCTAACCA NGG 1 0.6875 Tier III
18 NC_000072.6 6 51132225 - Gm32328 NNNGGTCTCATCCCTAACCA NGG 2 0.6016 Tier III
19 NC_000077.6 11 52655460 + Gm30366 NNNGGTACCATCCCTAAGCA NGG 2 0.1333 Tier III
20 NC_000068.7 2 66640417 - Gm35265 NNNGGTCCCATCCCTAACCA NGA 2 0.0477 Tier III
21 NC_000078.6 12 25148392 + Gm36287 NNNGGTGCCATCCCTGTCCA NGG 2 0.0256 Tier III
22 NC_000071.6 5 66658177 - Uchl1os NNNGGTGCCATCCCTAGGCA NGG 2 0.0235 Tier III
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Other clones with same target sequence:

(none)