Construct: sgRNA BRDN0001148405
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCTTGTAGGATAGCAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTN (7273)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76852
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178774101 |
+ |
TTN |
NNNCTTGTAGGATAGCAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
16002937 |
+ |
ABCC1 |
NNNCCTGTAGGGTAGCAATG |
NGG |
2 |
0.7222 |
Tier II |
| 3 |
NC_000005.10 |
5 |
38525348 |
- |
LIFR |
NNNCTTCTAGGATAGAAATG |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000002.12 |
2 |
189008416 |
- |
COL3A1 |
NNNCTAGTGGGATAGCAATG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000010.11 |
10 |
101038032 |
+ |
SFXN3 |
NNNTTTGTAGGAAAGCAATG |
NGG |
2 |
0.5538 |
Tier II |
| 6 |
NC_000010.11 |
10 |
14534790 |
+ |
FAM107B |
NNNCTTGTATTATAGCAATG |
NGG |
2 |
0.3125 |
Tier II |
| 7 |
NC_000001.11 |
1 |
57068066 |
- |
DAB1 |
NNNCTTGTAGGATTGCAGTG |
NGG |
2 |
0.2133 |
Tier II |
| 8 |
NC_000009.12 |
9 |
123783573 |
- |
DENND1A |
NNNCTTGTAGGATAGCTATG |
NGG |
1 |
0.1333 |
Tier II |
| 9 |
NC_000009.12 |
9 |
117243586 |
- |
ASTN2 |
NNNCTTGTAGGATAGTTATG |
NGG |
2 |
0.041 |
Tier II |
| 10 |
NC_000008.11 |
8 |
98283044 |
- |
NIPAL2 |
NNNCTTGTAGAATAGCAATG |
NTG |
2 |
0.039 |
Tier II |
| 11 |
NC_000002.12 |
2 |
140611326 |
+ |
LRP1B |
NNNCTTGTAGGATGGCAATG |
NGA |
2 |
0.0324 |
Tier II |
| 12 |
NC_000002.12 |
2 |
225869230 |
- |
LOC105373914 |
NNNCTTGTAAGATAGAAATG |
NGG |
2 |
0.9333 |
Tier III |
| 13 |
NC_000002.12 |
2 |
225869230 |
- |
LOC107985992 |
NNNCTTGTAAGATAGAAATG |
NGG |
2 |
0.9333 |
Tier III |
| 14 |
NC_000010.11 |
10 |
14534790 |
+ |
LOC124902381 |
NNNCTTGTATTATAGCAATG |
NGG |
2 |
0.3125 |
Tier III |
| 15 |
NC_000003.12 |
3 |
130256779 |
- |
COL6A4P2 |
NNNCTTGTAGCATAGCTATG |
NGG |
2 |
0.0571 |
Tier III |
| 16 |
NC_000008.11 |
8 |
98283044 |
- |
LOC105375659 |
NNNCTTGTAGAATAGCAATG |
NTG |
2 |
0.039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
61408287 |
+ |
Tle3 |
NNNCTTATAGGATAGCGATG |
NGG |
2 |
0.1765 |
Tier I |
| 2 |
NC_000068.7 |
2 |
77159022 |
- |
Ccdc141 |
NNNCTTGTAGGGTAGCAATG |
NGG |
1 |
0.7222 |
Tier II |
| 3 |
NC_000072.6 |
6 |
15195975 |
- |
Foxp2 |
NNNCTTCCAGGATAGCAATG |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000078.6 |
12 |
53090641 |
+ |
Akap6 |
NNNCTTGAAGGAGAGCAATG |
NGG |
2 |
0.2087 |
Tier II |
| 5 |
NC_000068.7 |
2 |
126406465 |
+ |
Atp8b4 |
NNNCTGGTAGGATAGCAAAG |
NGG |
2 |
0.1633 |
Tier II |
| 6 |
NC_000071.6 |
5 |
30498676 |
+ |
Cib4 |
NNNCTTGTAGGATTGCCATG |
NGG |
2 |
0.0941 |
Tier II |
| 7 |
NC_000081.6 |
15 |
16739573 |
- |
Cdh9 |
NNNCTTGTTGGATATCAATG |
NGG |
2 |
0.0857 |
Tier II |
| 8 |
NC_000073.6 |
7 |
87658288 |
- |
Grm5 |
NNNCTTGTGGGATAGCAATG |
NGA |
2 |
0.0463 |
Tier II |
| 9 |
NC_000072.6 |
6 |
110903442 |
- |
Grm7 |
NNNCTTGTAGGCTAGGAATG |
NGG |
2 |
0.0405 |
Tier II |
| 10 |
NC_000073.6 |
7 |
57312708 |
+ |
Gabrg3 |
NNNCTTGTAGGATATCACTG |
NGG |
2 |
0.0272 |
Tier II |
| 11 |
NC_000072.6 |
6 |
105987924 |
- |
Cntn4 |
NNNCTTGTAGGATATCAATG |
NGA |
2 |
0.0099 |
Tier II |
| 12 |
NC_000077.6 |
11 |
9464629 |
+ |
Abca13 |
NNNCTTGTAGGATATCAATG |
NGA |
2 |
0.0099 |
Tier II |
| 13 |
NC_000086.7 |
X |
113368404 |
- |
Dach2 |
NNNCTTGTAGGATATCAATG |
NGA |
2 |
0.0099 |
Tier II |
| 14 |
NC_000069.6 |
3 |
136952826 |
+ |
1700006H20Rik |
NNNCTTGTAGGATTGCATTG |
NGG |
2 |
0.2667 |
Tier III |
| 15 |
NC_000072.6 |
6 |
67722889 |
+ |
Igk |
NNNCTTGTAGGATATCAATG |
NGA |
2 |
0.0099 |
Tier III |
Other clones with same target sequence:
(none)
