Construct: sgRNA BRDN0001148441

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTCTGGAATATGCTCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AURKC (6795)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78056

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 57232617 + AURKC NNNCTGGAATATGCTCCAAG NGG 0 1.0 Tier I
2 NC_000010.11 10 127878171 - CLRN3 NNNATGGAATATGCTCCAAG NGA 2 0.0585 Tier I
3 NC_000001.11 1 201652756 + NAV1 NNNCTGGAAAATGTTCCAAG NGG 2 0.6286 Tier II
4 NC_000002.12 2 214336318 - SPAG16 NNNCTGGATTATGCTCCAAA NGG 2 0.5625 Tier II
5 NC_000003.12 3 173945408 + NLGN1 NNNCTGGAATGTGCTCTAAG NGG 2 0.3033 Tier II
6 NC_000003.12 3 143049579 - U2SURP NNNCTGGAAGATCCTCCAAG NGG 2 0.2105 Tier II
7 NC_000015.10 15 52362879 + MYO5A NNNCTGGAATATGCTCAGAG NGG 2 0.1867 Tier II
8 NC_000012.12 12 109728523 - FAM222A NNNCTGGAATATGCTGCAGG NGG 2 0.0577 Tier II
9 NC_000010.11 10 51453742 - PRKG1 NNNCTGGAATATTCTCCAAG NTG 2 0.0117 Tier II
10 NC_000023.11 X 123324915 + GRIA3 NNNCTGGAATATGCTCAAAG NGC 2 0.0104 Tier II
11 NC_000005.10 5 133443780 - FSTL4 NNNCTGGAATATGGTGCAAG NGG 2 0.0 Tier II
12 NC_000012.12 12 49922845 + LINC02395 NNNCTGGAATGGGCTCCAAG NGG 2 0.325 Tier III
13 NC_000013.11 13 34630839 - LINC00457 NNNCTGGGATATGCTCCAGG NGG 2 0.275 Tier III
14 NC_000012.12 12 41996809 - LOC105378247 NNNCAGGAATATGCTCCCAG NGG 2 0.0952 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 6997589 + Aurkc NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier I
2 NC_000073.6 7 135513961 - Clrn3 NNNGTGGAATATGCTCCAAG NGA 2 0.0347 Tier I
3 NC_000073.6 7 87797130 - Grm5 NNNCTGGAAAATGTTCCAAG NGG 2 0.6286 Tier II
4 NC_000075.6 9 58081191 + Stra6 NNNCTGGAAGATGCTCCAAG NGG 1 0.5 Tier II
5 NC_000075.6 9 58081191 + Ccdc33 NNNCTGGAAGATGCTCCAAG NGG 1 0.5 Tier II
6 NC_000084.6 18 35184038 - Ctnna1 NNNCTGGAATATACTCAAAG NGG 2 0.4308 Tier II
7 NC_000075.6 9 25526144 + Eepd1 NNNTTGGAATAGGCTCCAAG NGG 2 0.4 Tier II
8 NC_000083.6 17 8525629 - Pde10a NNNCTCGAATATGCTCCATG NGG 2 0.3671 Tier II
9 NC_000068.7 2 58299658 + Acvr1c NNNCTGGAATATTCTCCAAG NGG 1 0.3 Tier II
10 NC_000068.7 2 24676796 + Cacna1b NNNCTTGAATATCCTCCAAG NGG 2 0.2807 Tier II
11 NC_000071.6 5 26919700 + Dpp6 NNNCTTGAATATGCTCCAGG NGG 2 0.25 Tier II
12 NC_000077.6 11 57756950 + Galnt10 NNNCTGGAATGTGCTCCAGG NGG 2 0.2438 Tier II
13 NC_000078.6 12 28469685 + Dcdc2c NNNCTGGAATATGCTTTAAG NGG 2 0.1436 Tier II
14 NC_000076.6 10 114523906 - Trhde NNNCTGGAATAGGCTCCACG NGG 2 0.1034 Tier II
15 NC_000080.6 14 74850600 - Lrch1 NNNCTAGAATATGCTCCAAG NGA 2 0.0694 Tier II
16 NC_000085.6 19 25037389 - Dock8 NNNGTGGAATATGCTCCAAG NGT 2 0.0081 Tier II
17 NC_000076.6 10 44177301 - Speer5-ps1 NNNCTGGAAGATGCTCCAAT NGG 2 0.35 Tier III
18 NC_000070.6 4 44752836 - Gm12463 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
19 NC_000073.6 7 10813440 + Gm7203 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
20 NC_000073.6 7 10994051 - Gm7789 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
21 NC_000073.6 7 11083943 + Gm7151 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
22 NC_000073.6 7 11183729 + Gm6909 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
23 NC_000073.6 7 11234483 + Gm39481 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
24 NC_000073.6 7 11386260 - Gm45598 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
25 NC_000073.6 7 11466420 - Gm7207 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
26 NC_000073.6 7 11592059 - Gm7210 NNNCTGGAGTATGCTCCAGG NGG 2 0.25 Tier III
27 NC_000073.6 7 70716413 - Gm39031 NNNCTGGAATATGCTCTAAG NGA 2 0.0324 Tier III
Download CSV

Other clones with same target sequence:

(none)

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