Construct: sgRNA BRDN0001148509

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGAAAAGTGCAAATATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIP5K2 (23262)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76339

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 103159211 + PPIP5K2 NNNAAAAGTGCAAATATGAA NGG 0 1.0 Tier I
2 NC_000002.12 2 241057994 + SNED1 NNNAAAAATACAAATATGAA NGG 2 0.9333 Tier II
3 NC_000002.12 2 241057994 + MTERF4 NNNAAAAATACAAATATGAA NGG 2 0.9333 Tier II
4 NC_000014.9 14 64220020 - SYNE2 NNNAAAAGTACAAATATGAA NGG 1 0.9333 Tier II
5 NC_000001.11 1 219397981 - LYPLAL1 NNNAAAAGTAAAAATATGAA NGG 2 0.7 Tier II
6 NC_000011.10 11 47502449 + CELF1 NNNAAAAATGCAAATATTAA NGG 2 0.6667 Tier II
7 NC_000012.12 12 64730428 + GNS NNNAAAAGTACAGATATGAA NGG 2 0.6087 Tier II
8 NC_000006.12 6 145920724 + SHPRH NNNTAAAGTACAAATATGAA NGG 2 0.5939 Tier II
9 NC_000023.11 X 133693955 - GPC3 NNNAAAAGGACAAATATGAA NGG 2 0.5778 Tier II
10 NC_000023.11 X 124502576 - TENM1 NNNAAAAGTACAAAAATGAA NGG 2 0.5404 Tier II
11 NC_000013.11 13 87662314 - LOC124900338 NNNAAAAATGCAAATATGTA NGG 2 0.5385 Tier II
12 NC_000006.12 6 51831272 + PKHD1 NNNAAAAATGCAAATAAGAA NGG 2 0.5333 Tier II
13 NC_000023.11 X 37780966 + CYBB NNNAAAAGTACAATTATGAA NGG 2 0.4978 Tier II
14 NC_000003.12 3 114455410 + ZBTB20 NNNAAAAGTGCAAATACAAA NGG 2 0.4887 Tier II
15 NC_000004.12 4 174731546 + GLRA3 NNNAAAAGTTCAAATATGAT NGG 2 0.4875 Tier II
16 NC_000015.10 15 71839107 - MYO9A NNNAAAAGTGCAAATAAAAA NGG 2 0.3692 Tier II
17 NC_000008.11 8 88054274 - MMP16 NNNAAAAGGGCAAAAATGAA NGG 2 0.3584 Tier II
18 NC_000002.12 2 168603255 - CERS6 NNNAAATGTTCAAATATGAA NGG 2 0.3555 Tier II
19 NC_000003.12 3 62053945 + PTPRG NNNAAATGTTCAAATATGAA NGG 2 0.3555 Tier II
20 NC_000009.12 9 111917476 - UGCG NNNCAAAATGCAAATATGAA NGG 2 0.3529 Tier II
21 NC_000003.12 3 122013606 + ILDR1 NNNAACAGTGCAAATATGAG NGG 2 0.3476 Tier II
22 NC_000004.12 4 163640519 + MARCHF1 NNNACAAGTGCAAATATAAA NGG 2 0.3462 Tier II
23 NC_000004.12 4 101085339 - PPP3CA NNNAAAAGGGCAAATAAGAA NGG 2 0.3302 Tier II
24 NC_000003.12 3 180616796 + TTC14 NNNAAAAATGTAAATATGAA NGG 2 0.3077 Tier II
25 NC_000003.12 3 180616796 + CCDC39 NNNAAAAATGTAAATATGAA NGG 2 0.3077 Tier II
26 NC_000023.11 X 114748568 + HTR2C NNNAAAAGTGCATATATGAA NGG 1 0.3 Tier II
27 NC_000007.14 7 87363016 + CROT NNNTAATGTGCAAATATGAA NGG 2 0.2784 Tier II
28 NC_000001.11 1 185881183 - HMCN1 NNNATGAGTGCAAATATGAA NGG 2 0.2597 Tier II
29 NC_000016.10 16 23340092 - SCNN1B NNNAAAAGAGGAAATATGAA NGG 2 0.2321 Tier II
30 NC_000006.12 6 45335018 + RUNX2 NNNATAAGGGCAAATATGAA NGG 2 0.2251 Tier II
31 NC_000006.12 6 45335018 + SUPT3H NNNATAAGGGCAAATATGAA NGG 2 0.2251 Tier II
32 NC_000012.12 12 66593691 + GRIP1 NNNGAAAGTGCTAATATGAA NGG 2 0.2083 Tier II
33 NC_000018.10 18 21298032 + GREB1L NNNAAAAGTCCAAGTATGAA NGG 2 0.1867 Tier II
34 NC_000007.14 7 38983153 + POU6F2 NNNTAAAGTGCAAACATGAA NGG 2 0.1736 Tier II
35 NC_000013.11 13 71814667 + DACH1 NNNAAAAGTGGAAATATTAA NGG 2 0.1667 Tier II
36 NC_000008.11 8 140574642 + AGO2 NNNTAAAGTGCAAATATGAA NAG 2 0.165 Tier II
37 NC_000017.11 17 64481989 + POLG2 NNNAAAAGTGAAACTATGAA NGG 2 0.1607 Tier II
38 NC_000017.11 17 64481989 + MILR1 NNNAAAAGTGAAACTATGAA NGG 2 0.1607 Tier II
39 NC_000001.11 1 245254602 + KIF26B NNNAAAAGTGCATATAAGAA NGG 2 0.16 Tier II
40 NC_000004.12 4 170071892 - AADAT NNNAAGAGTGCAACTATGAA NGG 2 0.1531 Tier II
41 NC_000007.14 7 151372582 + NUB1 NNNAAAAGTGCAAATGTGAG NGG 2 0.1471 Tier II
42 NC_000007.14 7 151372582 + WDR86 NNNAAAAGTGCAAATGTGAG NGG 2 0.1471 Tier II
43 NC_000013.11 13 32080835 + FRY NNNAAAAGTGCAAAAATGAC NGG 2 0.1316 Tier II
44 NC_000011.10 11 92628426 - FAT3 NNNAAAAGGGCAAATGTGAA NGG 2 0.119 Tier II
45 NC_000002.12 2 233981128 - TRPM8 NNNATAAGTGTAAATATGAA NGG 2 0.1119 Tier II
46 NC_000004.12 4 92773870 - GRID2 NNNAAATGTGCAAATATGAC NGG 2 0.0994 Tier II
47 NC_000001.11 1 93460262 - FNBP1L NNNAAAAGGGCAAATAGGAA NGG 2 0.0728 Tier II
48 NC_000008.11 8 99502321 + VPS13B NNNAAAAGTGCAAATATTAA NCG 2 0.0714 Tier II
49 NC_000003.12 3 101786005 + NXPE3 NNNAAAAGTGCAAAAAGGAA NGG 2 0.0681 Tier II
50 NC_000016.10 16 52533176 + TOX3 NNNAAAAGTGTAAATGTGAA NGG 2 0.0592 Tier II
51 NC_000002.12 2 74595981 + M1AP NNNAAAAATGCAAAGATGAA NGG 2 0.05 Tier II
52 NC_000005.10 5 66881024 + MAST4 NNNAAAAGTGCAAATATTAA NGA 2 0.0463 Tier II
53 NC_000006.12 6 107957218 - SEC63 NNNAAAAGTGCAAGTATGAA NGA 2 0.0324 Tier II
54 NC_000002.12 2 151707569 - NEB NNNAAAAGTGCAAATAGGAA NAG 2 0.0305 Tier II
55 NC_000013.11 13 100161650 - PCCA NNNAAAAGTCCAAATATGAA NGA 2 0.0278 Tier II
56 NC_000013.11 13 51769388 + DHRS12 NNNAAAAGTGCAAAAATGAA NTG 2 0.0226 Tier II
57 NC_000012.12 12 129869521 - TMEM132D NNNAAAAATGCAAATATGAA NGC 2 0.0222 Tier II
58 NC_000009.12 9 42028052 - CNTNAP3B NNNAAAAGTGTAAATATGAA NGA 2 0.0214 Tier II
59 NC_000013.11 13 23216380 + SGCG NNNAAAAGTGTAAATATGAA NGA 2 0.0214 Tier II
60 NC_000015.10 15 76351616 + SCAPER NNNAAAAGTACAAATATGAA NGC 2 0.0207 Tier II
61 NC_000021.9 21 33562070 - SON NNNAAAAGTGCAGATATGAA NGC 2 0.0145 Tier II
62 NC_000003.12 3 40113649 + MYRIP NNNAAAAGTGAAAATATGAA NGT 2 0.0121 Tier II
63 NC_000006.12 6 53906710 + LRRC1 NNNAAAAGTGAAAATATGAA NGT 2 0.0121 Tier II
64 NC_000001.11 1 43341499 + MPL NNNAAAAGTGCAAAAATGAA NGT 2 0.0093 Tier II
65 NC_000023.11 X 31819916 - DMD NNNAAAAGTGCAACTATGAA NTG 2 0.0083 Tier II
66 NC_000017.11 17 81155055 - AATK NNNAAAAGTGCAAAGATGAA NGA 2 0.0035 Tier II
67 NC_000022.11 22 33123543 - LARGE1 NNNAAAAGTGAAAATTTGAA NGG 2 0.0 Tier II
68 NC_000006.12 6 133959884 + TBPL1 NNNAAAAGTGCAAATATGAA NAT 2 0.0 Tier II
69 NC_000006.12 6 133959884 + LOC128092253 NNNAAAAGTGCAAATATGAA NAT 2 0.0 Tier II
70 NC_000023.11 X 65014589 - ZC4H2 NNNAAAAGTGCAAATATGAA NAC 2 0.0 Tier II
71 NC_000002.12 2 241057994 + SNED1-AS1 NNNAAAAATACAAATATGAA NGG 2 0.9333 Tier III
72 NC_000013.11 13 87662314 - MIR4500HG NNNAAAAATGCAAATATGTA NGG 2 0.5385 Tier III
73 NC_000002.12 2 195302712 + LOC105376755 NNNAAAATTTCAAATATGAA NGG 2 0.5078 Tier III
74 NC_000006.12 6 9818381 + OFCC1 NNNAAAACTTCAAATATGAA NGG 2 0.5 Tier III
75 NC_000014.9 14 30483972 - G2E3-AS1 NNNAAAAGTACAAATAAGAA NGG 2 0.4978 Tier III
76 NC_000003.12 3 114455410 + ZBTB20-AS5 NNNAAAAGTGCAAATACAAA NGG 2 0.4887 Tier III
77 NC_000007.14 7 17157896 - LOC101927609 NNNAGAAGTGCAAAAATGAA NGG 2 0.4168 Tier III
78 NC_000007.14 7 17157896 - LOC107986772 NNNAGAAGTGCAAAAATGAA NGG 2 0.4168 Tier III
79 NC_000007.14 7 130948883 + LINC-PINT NNNAAAAGTGAAAATAAGAA NGG 2 0.4 Tier III
80 NC_000015.10 15 45864659 - LOC105370802 NNNGAAAGTGCAAAAATGAA NGG 2 0.3618 Tier III
81 NC_000015.10 15 45864659 - LOC107984794 NNNGAAAGTGCAAAAATGAA NGG 2 0.3618 Tier III
82 NC_000006.12 6 1376181 + LINC01394 NNNAAAAGTGCAAATAATAA NGG 2 0.3556 Tier III
83 NC_000023.11 X 114748568 + LOC105373313 NNNAAAAGTGCATATATGAA NGG 1 0.3 Tier III
84 NC_000004.12 4 104299729 + LOC105377350 NNNAAATGGGCAAATATGAA NGG 2 0.2708 Tier III
85 NC_000015.10 15 39346997 - LOC105370777 NNNAAACCTGCAAATATGAA NGG 2 0.2692 Tier III
86 NC_000007.14 7 38983153 + POU6F2-AS2 NNNTAAAGTGCAAACATGAA NGG 2 0.1736 Tier III
87 NC_000002.12 2 72026387 + LOC105374798 NNNAAAAGTGGAAATATAAA NGG 2 0.1731 Tier III
88 NC_000011.10 11 41866690 - LOC105376639 NNNAAAAGTGCACATATGCA NGG 2 0.0436 Tier III
89 NC_000009.12 9 67159368 + CNTNAP3P2 NNNAAAAGTGTAAATATGAA NGA 2 0.0214 Tier III
90 NC_000002.12 2 34273836 - LINC01317 NNNAAAACTGCAAATTTGAA NGG 2 0.0 Tier III
91 NC_000016.10 16 49340675 - LOC124903689 NNNAAAATTGCAAATTTGAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 97740580 - Ppip5k2 NNNAAAAGTGCAAACATGAA NGG 1 0.2727 Tier I
2 NC_000068.7 2 51962594 + Nmi NNNAAAGGTACAAATATGAA NGG 2 0.6588 Tier II
3 NC_000079.6 13 104756035 + Cwc27 NNNGAAAGTGCAAATATGAA NGG 1 0.625 Tier II
4 NC_000075.6 9 111355440 + Trank1 NNNAAAAGTGAAAATATAAA NGG 2 0.5192 Tier II
5 NC_000081.6 15 10608208 - Rai14 NNNTACAGTGCAAATATGAA NGG 2 0.2893 Tier II
6 NC_000071.6 5 137424768 - Zan NNNAAACGTGCAGATATGAA NGG 2 0.2853 Tier II
7 NC_000082.6 16 6558456 - Rbfox1 NNNAACAGTGCAAAAATGAA NGG 2 0.2632 Tier II
8 NC_000068.7 2 126458974 + Atp8b4 NNNAAAAATGCAAATATGAA NAG 2 0.2593 Tier II
9 NC_000083.6 17 56202081 + Dpp9 NNNAAAAGTGCAAATAACAA NGG 2 0.254 Tier II
10 NC_000072.6 6 63465967 - Grid2 NNNAAAAGGCCAAATATGAA NGG 2 0.2476 Tier II
11 NC_000084.6 18 57543240 + Ccdc192 NNNAAAAATGCAAATATGCA NGG 2 0.2069 Tier II
12 NC_000073.6 7 78522848 - Ntrk3 NNNAAAAGTGCTAAAATGAA NGG 2 0.193 Tier II
13 NC_000086.7 X 59166893 + Fgf13 NNNAAAAATGCAAATGTGAA NGG 2 0.1923 Tier II
14 NC_000080.6 14 98122629 + Dach1 NNNAAAAGTGGAAATATTAA NGG 2 0.1667 Tier II
15 NC_000084.6 18 58587220 - Slc27a6 NNNATATGTGCAAATATGAA NGG 2 0.1591 Tier II
16 NC_000067.6 1 87693620 - Inpp5d NNNACAAGTGCCAATATGAA NGG 2 0.1316 Tier II
17 NC_000076.6 10 9436520 + Samd5 NNNCAAAGTGCCAATATGAA NGG 2 0.0929 Tier II
18 NC_000077.6 11 29382460 - Ccdc88a NNNAAATGTGCAAATATGCA NGG 2 0.0905 Tier II
19 NC_000075.6 9 65359486 + Pdcd7 NNNAAAAGAGCAAATATGAA NGA 2 0.0645 Tier II
20 NC_000079.6 13 9482271 - Dip2c NNNAAAAGTGCACATATGAA NAG 2 0.0546 Tier II
21 NC_000086.7 X 84780373 + Dmd NNNAAAAGTACAAAGATGAA NGG 2 0.0467 Tier II
22 NC_000077.6 11 54124921 - P4ha2 NNNAAAAGAGCAAAGATGAA NGG 2 0.0464 Tier II
23 NC_000079.6 13 83534019 - Mef2c NNNAAAAGTGCAAATGTGCA NGG 2 0.0398 Tier II
24 NC_000081.6 15 30940561 - Ctnnd2 NNNAAAAGTGAAAAGATGAA NGG 2 0.0375 Tier II
25 NC_000086.7 X 114494959 - Klhl4 NNNAAAAGAGCAAATATGAA NTG 2 0.0362 Tier II
26 NC_000068.7 2 69568414 + Lrp2 NNNAAATGTGCAAAGATGAA NGG 2 0.0219 Tier II
27 NC_000078.6 12 82989042 - Rgs6 NNNAAAAGTGCAAATGTGAA NGA 2 0.0134 Tier II
28 NC_000079.6 13 63198131 - Aopep NNNAAAAGTTCAAATATGAA NGT 2 0.0131 Tier II
29 NC_000067.6 1 4153875 + Rp1 NNNAAAAGTGCAAAGATGAA NAG 2 0.013 Tier II
30 NC_000071.6 5 58037794 + Pcdh7 NNNAAAAGTGCAAAGATGAA NAG 2 0.013 Tier II
31 NC_000079.6 13 85284491 - Rasa1 NNNAAAAGTGTAAATATGAA NTG 2 0.012 Tier II
32 NC_000077.6 11 53721695 + Il5 NNNAAAAGTGCAATTATGAA NGT 2 0.0086 Tier II
33 NC_000082.6 16 58671174 - Cpox NNNAAAAGTGCAACTATGAA NTG 2 0.0083 Tier II
34 NC_000069.6 3 116678875 + Slc35a3 NNNAAAAGTGCAAATGTGAA NTG 2 0.0075 Tier II
35 NC_000071.6 5 74546609 - Fip1l1 NNNAAAAGTGCACATATGAA NGC 2 0.0047 Tier II
36 NC_000074.6 8 13167406 + Lamp1 NNNAAAAATGCAAATCTGAA NGG 2 0.0 Tier II
37 NC_000068.7 2 4193872 + Frmd4a NNNAAAAGTGCAAATCTGGA NGG 2 0.0 Tier II
38 NC_000076.6 10 91423833 + Gm40736 NNNAAAAATGCAAATATTAA NGG 2 0.6667 Tier III
39 NC_000068.7 2 138600171 + Gm14061 NNNAAAATTGAAAATATGAA NGG 2 0.4688 Tier III
40 NC_000073.6 7 94652426 + Gm32647 NNNAAAAGTGCAAATAAGAG NGG 2 0.4078 Tier III
41 NC_000072.6 6 68255557 - Igk NNNATAAGTGCAAATATGAA NGG 1 0.3636 Tier III
42 NC_000067.6 1 184551143 - 1700112H15Rik NNNATAAGTTCAAATATGAA NGG 2 0.2955 Tier III
43 NC_000081.6 15 10608208 - Gm10389 NNNTACAGTGCAAATATGAA NGG 2 0.2893 Tier III
44 NC_000076.6 10 24357185 + Gm15270 NNNAAAAGTGCAAATATCAT NGG 2 0.2857 Tier III
45 NC_000073.6 7 61775080 - A230057D06Rik NNNAACAGTGCAAAAATGAA NGG 2 0.2632 Tier III
46 NC_000069.6 3 28758085 + 1700112D23Rik NNNAAAAATGCAAATGTGAA NGG 2 0.1923 Tier III
47 NC_000076.6 10 112781008 - 1700010J16Rik NNNAAAACTGCAAATATGAA NAG 2 0.1595 Tier III
48 NC_000072.6 6 67789589 - Igk NNNATATGTGCAAATATGAA NGG 2 0.1591 Tier III
49 NC_000072.6 6 67956064 + Igk NNNATATGTGCAAATATGAA NGG 2 0.1591 Tier III
50 NC_000084.6 18 39870867 - Gm41707 NNNAAAAGTGCAAACAAGAA NGG 2 0.1455 Tier III
51 NC_000077.6 11 28834166 + Gm34185 NNNAAAAGTGCATATATGAA NAG 2 0.0778 Tier III
52 NC_000067.6 1 130519100 + Gm16083 NNNAAAAATGCAAATATGAA NTG 2 0.039 Tier III
53 NC_000081.6 15 30940561 - 9630009A06Rik NNNAAAAGTGAAAAGATGAA NGG 2 0.0375 Tier III
54 NC_000085.6 19 33310604 + Rnls NNNAAAAGTGAAAATATGAA NTG 2 0.0292 Tier III
55 NC_000067.6 1 168685517 - Gm34320 NNNAATAGTGCAAATATGAA NTG 2 0.0278 Tier III
56 NC_000080.6 14 27407538 - Gm52095 NNNAAAAGTGCATATATGAA NGC 2 0.0067 Tier III
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Other clones with same target sequence:

(none)