Construct: sgRNA BRDN0001148530

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTTTGCTAAGAGCAAGTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1G (57172)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75984

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 209611547 + CAMK1G NNNTTGCTAAGAGCAAGTGG NGG 0 1.0 Tier I
2 NC_000003.12 3 9759494 - CAMK1 NNNTTGCCAAGAGCAAGTGG NAG 2 0.2593 Tier I
3 NC_000012.12 12 121953923 - CFAP251 NNNTTGCTAAGAGCAAGTGA NGG 1 0.9375 Tier II
4 NC_000015.10 15 75626211 - SNUPN NNNTTGTTAAGGGCAAGTGG NGG 2 0.5868 Tier II
5 NC_000016.10 16 85436225 - GSE1 NNNTTGCTAAGAGCAAGGGA NGG 2 0.3125 Tier II
6 NC_000003.12 3 9759494 - OGG1 NNNTTGCCAAGAGCAAGTGG NAG 2 0.2593 Tier II
7 NC_000001.11 1 46303083 - LRRC41 NNNTGGCTAAGAGCAAGGGG NGG 2 0.2133 Tier II
8 NC_000001.11 1 205669090 + SLC45A3 NNNTGGCTAAGAGCAAGGGG NGG 2 0.2133 Tier II
9 NC_000009.12 9 68817601 + PIP5K1B NNNTTGATAAGAGCAAGTGG NAG 2 0.1944 Tier II
10 NC_000007.14 7 150035793 + ACTR3C NNNTTCCTAAGAGCCAGTGG NGG 2 0.186 Tier II
11 NC_000011.10 11 120852394 + GRIK4 NNNTTGCTAAGCGCAAGTGT NGG 2 0.1842 Tier II
12 NC_000001.11 1 12026347 + MIIP NNNTTGCTGAGAGCAACTGG NGG 2 0.1569 Tier II
13 NC_000001.11 1 46690903 + EFCAB14 NNNTTGCAAAGAGCAGGTGG NGG 2 0.1538 Tier II
14 NC_000008.11 8 74465623 - GDAP1 NNNATGCTAAGAGCAATTGG NGG 2 0.119 Tier II
15 NC_000015.10 15 89117685 + ABHD2 NNNTTGCTAAGACCCAGTGG NGG 2 0.1148 Tier II
16 NC_000022.11 22 48555126 - TAFA5 NNNTTGCTAAGAGACAGTGG NGG 2 0.0955 Tier II
17 NC_000003.12 3 129088463 + RAB43 NNNTTGCTAAGAGCCAGGGG NGG 2 0.0909 Tier II
18 NC_000003.12 3 129088463 + ISY1-RAB43 NNNTTGCTAAGAGCCAGGGG NGG 2 0.0909 Tier II
19 NC_000022.11 22 50558684 + SYCE3 NNNTTGCTAAGAGTAAGTGG NGA 2 0.0509 Tier II
20 NC_000003.12 3 151476825 + IGSF10 NNNTTTCTAAGAGCAAGTGG NGA 2 0.0463 Tier II
21 NC_000023.11 X 12099553 - FRMPD4 NNNTTGCTAAGATCAAGTGG NGA 2 0.0208 Tier II
22 NC_000002.12 2 27448730 - IFT172 NNNTTGCTAAGAGCATGGGG NGG 2 0.0 Tier II
23 NC_000008.11 8 72243131 + TRPA2P NNNTTGGTAAGAGCAAGTAG NGG 2 0.3361 Tier III
24 NC_000008.11 8 128163832 - LOC124902020 NNNTTGCCAAGAGCAAGGGG NGG 2 0.3333 Tier III
25 NC_000001.11 1 20374140 - LINC01141 NNNTTGCTAAGAGCAGATGG NGG 2 0.1795 Tier III
26 NC_000015.10 15 47897314 + LOC124900354 NNNTTGCTAAGAGCAGATGG NGG 2 0.1795 Tier III
27 NC_000001.11 1 46690903 + EFCAB14-AS1 NNNTTGCAAAGAGCAGGTGG NGG 2 0.1538 Tier III
28 NC_000016.10 16 46623765 + RAB43P1 NNNTTGCTAAGAGCCAGGGG NGG 2 0.0909 Tier III
29 NC_000011.10 11 109742435 + LINC02715 NNNTAGCTAAGAGCATGTGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 193348725 - Camk1g NNNTTGCCAAGAGCAAGTGG NGG 1 1.0 Tier I
2 NC_000072.6 6 113336180 - Camk1 NNNTTGCCAAGAGCAAGTGG NAG 2 0.2593 Tier I
3 NC_000067.6 1 53683992 - Dnah7a NNNTTACGAAGAGCAAGTGG NGG 2 0.7333 Tier II
4 NC_000076.6 10 123490338 + Tafa2 NNNTTGCTAAAAGTAAGTGG NGG 2 0.7333 Tier II
5 NC_000076.6 10 53042984 + Slc35f1 NNNGTGCTAAGAACAAGTGG NGG 2 0.5769 Tier II
6 NC_000085.6 19 24417082 - Pip5k1b NNNTTGCTTAGAGCAAATGG NGG 2 0.56 Tier II
7 NC_000067.6 1 91207614 - Ramp1 NNNTTGCAAAGAGCAAGAGG NGG 2 0.5333 Tier II
8 NC_000073.6 7 124175885 + Hs3st4 NNNTTTCAAAGAGCAAGTGG NGG 2 0.5333 Tier II
9 NC_000084.6 18 69519708 + Tcf4 NNNTTGTTAAGAGAAAGTGG NGG 2 0.2844 Tier II
10 NC_000068.7 2 150991165 - Ninl NNNTTGCTCACAGCAAGTGG NGG 2 0.2449 Tier II
11 NC_000080.6 14 27821518 + Erc2 NNNTTTCTAAGAGCAAGGGG NGG 2 0.2222 Tier II
12 NC_000067.6 1 39374064 + Tbc1d8 NNNTTGCTAAGAGCCAGTAG NGG 2 0.1948 Tier II
13 NC_000079.6 13 15700622 + Gli3 NNNTTGCTGAGAGCAGGTGG NGG 2 0.1282 Tier II
14 NC_000081.6 15 91357994 + Slc2a13 NNNTTGCTCAGAGCAAGTGG NGA 2 0.0397 Tier II
15 NC_000073.6 7 79450747 - Polg NNNTTGCTAAGAGCAAGTGA NTG 2 0.0365 Tier II
16 NC_000081.6 15 64055476 + Cyrib NNNTTCCTAAGAGCAAGTGG NTG 2 0.0266 Tier II
17 NC_000075.6 9 99215333 + Cep70 NNNTTGCTAAGAGCAAGTGG NCA 2 0.0 Tier II
18 NC_000067.6 1 93238318 + Sned1 NNNTTGCTAAGAGCATGTGG NGT 2 0.0 Tier II
19 NC_000081.6 15 12626024 + Pdzd2 NNNTTGCTAAGGGGAAGTGG NGG 2 0.0 Tier II
20 NC_000085.6 19 24417082 - Pip5k1bos NNNTTGCTTAGAGCAAATGG NGG 2 0.56 Tier III
21 NC_000075.6 9 68486434 - 4930502A04Rik NNNTTGCTTGGAGCAAGTGG NGG 2 0.3333 Tier III
22 NC_000085.6 19 26488362 + Gm34808 NNNATGCTAAGAGAAAGTGG NGG 2 0.1667 Tier III
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Other clones with same target sequence:

(none)