Construct: sgRNA BRDN0001148542

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTCATCAAACTAAGACACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKMT2 (1160)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76597

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 81254437 + CKMT2 NNNATCAAACTAAGACACAA NGG 0 1.0 Tier I
2 NC_000005.10 5 33651565 - ADAMTS12 NNNATCAAACTAAGAAACAT NGG 2 0.6 Tier II
3 NC_000003.12 3 118921636 + IGSF11 NNNAGAAAACTAAGACACAA NGG 2 0.5943 Tier II
4 NC_000001.11 1 14324602 + KAZN NNNATCAAAATAAGACATAA NGG 2 0.5571 Tier II
5 NC_000016.10 16 79259448 - MAF NNNATCAAACAAAGACATAA NGG 2 0.4821 Tier II
6 NC_000002.12 2 141267212 + LRP1B NNNAATAAACTAAGACACAA NGG 2 0.4643 Tier II
7 NC_000009.12 9 117187828 + ASTN2 NNNAACAGACTAAGACACAA NGG 2 0.3667 Tier II
8 NC_000001.11 1 114676524 + AMPD1 NNNATGAAACTAAGGCACAA NGG 2 0.325 Tier II
9 NC_000007.14 7 95775154 + DYNC1I1 NNNAGGAAACTAAGACACAA NGG 2 0.32 Tier II
10 NC_000019.10 19 46337856 - HIF3A NNNATCAAATTATGACACAA NGG 2 0.2824 Tier II
11 NC_000020.11 20 13558151 + TASP1 NNNATAAAACTATGACACAA NGG 2 0.2786 Tier II
12 NC_000023.11 X 14492752 - GLRA2 NNNAACAAACTAAGACACTA NGG 2 0.2692 Tier II
13 NC_000009.12 9 116527211 + ASTN2 NNNATCAAACTAAAATACAA NGG 2 0.2308 Tier II
14 NC_000009.12 9 137652384 - EHMT1 NNNATAAAACTAAGACACAC NGG 2 0.211 Tier II
15 NC_000018.10 18 9522902 + RALBP1 NNNATCAAACTCAGGCACAA NGG 2 0.1711 Tier II
16 NC_000009.12 9 75073427 + NMRK1 NNNAGCAAACTAATACACAA NGG 2 0.1707 Tier II
17 NC_000002.12 2 227901335 - DAW1 NNNATCAATCTAAGACACAA NAG 2 0.1556 Tier II
18 NC_000009.12 9 91254927 + AUH NNNATAAAACTAAGAGACAA NGG 2 0.1429 Tier II
19 NC_000003.12 3 174639363 + NAALADL2 NNNATCAAACTAAGACCCAG NGG 2 0.1349 Tier II
20 NC_000003.12 3 169482245 + MECOM NNNATGAAACTAAGACACAC NGG 2 0.1136 Tier II
21 NC_000023.11 X 32298719 + DMD NNNATCAAACTTTGACACAA NGG 2 0.1 Tier II
22 NC_000003.12 3 25229223 - RARB NNNATCAAACTAAGAAACAA NGA 2 0.0694 Tier II
23 NC_000013.11 13 74144407 + KLF12 NNNATGAAACTAAGACACAA NGC 2 0.0111 Tier II
24 NC_000001.11 1 42012981 - HIVEP3 NNNATCAAACTAATACACAA NGC 2 0.0059 Tier II
25 NC_000005.10 5 81254437 + CKMT2-AS1 NNNATCAAACTAAGACACAA NGG 0 1.0 Tier III
26 NC_000015.10 15 25130897 - SNHG14 NNNATCAAATTAAAACACAA NGG 2 0.7059 Tier III
27 NC_000008.11 8 108964603 + LOC107984017 NNNAGCAAATTAAGACACAA NGG 2 0.6024 Tier III
28 NC_000006.12 6 9790040 + OFCC1 NNNAACAAACTAAGACACAA NGG 1 0.5 Tier III
29 NC_000007.14 7 135999800 + LOC105375523 NNNAGCAAACTGAGACACAA NGG 2 0.4622 Tier III
30 NC_000006.12 6 106736104 + LINC02532 NNNAACAGACTAAGACACAA NGG 2 0.3667 Tier III
31 NC_000009.12 9 118910307 + LOC101928849 NNNATCATACTGAGACACAA NGG 2 0.3095 Tier III
32 NC_000005.10 5 174810330 - LOC105377742 NNNAGCAAACTAACACACAA NGG 2 0.2743 Tier III
33 NC_000009.12 9 116527211 + ASTN2-AS1 NNNATCAAACTAAAATACAA NGG 2 0.2308 Tier III
34 NC_000012.12 12 22817838 - LINC02955 NNNATGACACTAAGACACAA NGG 2 0.2143 Tier III
35 NC_000024.10 Y 19073403 + TTTY14 NNNATTAAACTAAGACCCAA NGG 2 0.1639 Tier III
36 NC_000006.12 6 81935941 + LINC02542 NNNATAAAACTAAGACTCAA NGG 2 0.1238 Tier III
37 NC_000010.11 10 64068018 + LOC124902439 NNNAACAAACTAAGACACCA NGG 2 0.1034 Tier III
38 NC_000005.10 5 17783702 - LOC105374666 NNNATCAAACTAAGAGAAAA NGG 2 0.0828 Tier III
39 NC_000005.10 5 69004411 + LINC02198 NNNATCAAACTTAGAGACAA NGG 2 0.0513 Tier III
40 NC_000005.10 5 69004411 + LOC107984116 NNNATCAAACTTAGAGACAA NGG 2 0.0513 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 91861808 - Ckmt2 NNNATCAAACTAAGGCACAA NGG 1 0.65 Tier I
2 NC_000076.6 10 102126200 - Mgat4c NNNATCAAACAAAGAAACAA NGG 2 0.75 Tier II
3 NC_000082.6 16 34956863 - Mylk NNNAGAAAACTAAGACACAA NGG 2 0.5943 Tier II
4 NC_000080.6 14 10003669 + Fhit NNNATCAAACTGAGACACAG NGG 2 0.5523 Tier II
5 NC_000073.6 7 66699707 - Lins1 NNNACCAAACTAAGACACTA NGG 2 0.5385 Tier II
6 NC_000080.6 14 87101687 - Diaph3 NNNATCAAACCAAAACACAA NGG 2 0.5 Tier II
7 NC_000070.6 4 154559082 + Prdm16 NNNATCTAACTAAGACACAG NGG 2 0.3346 Tier II
8 NC_000068.7 2 3455915 + Suv39h2 NNNATGAAACTAAGGCACAA NGG 2 0.325 Tier II
9 NC_000068.7 2 3455915 + Dclre1c NNNATGAAACTAAGGCACAA NGG 2 0.325 Tier II
10 NC_000073.6 7 57322591 + Gabrg3 NNNATCAAAGTGAGACACAA NGG 2 0.2809 Tier II
11 NC_000077.6 11 110150141 + Abca9 NNNAACTAACTAAGACACAA NGG 2 0.2188 Tier II
12 NC_000072.6 6 135161224 + Hebp1 NNNATCAAACTAAGAACCAA NGG 2 0.1765 Tier II
13 NC_000084.6 18 65595193 - Zfp532 NNNAACAAACTAAGCCACAA NGG 2 0.1364 Tier II
14 NC_000082.6 16 36061950 + Fam162a NNNGTCAAACTAAGACACCA NGG 2 0.1293 Tier II
15 NC_000076.6 10 74206637 + Pcdh15 NNNATCAAAATAAGACAGAA NGG 2 0.1156 Tier II
16 NC_000086.7 X 139930157 - Rbm41 NNNAACAAACTAAGACACCA NGG 2 0.1034 Tier II
17 NC_000072.6 6 94525126 - Slc25a26 NNNATCACACTAAGACACAA NCG 2 0.0459 Tier II
18 NC_000068.7 2 48906370 - Orc4 NNNATCAAACTAATAGACAA NGG 2 0.041 Tier II
19 NC_000072.6 6 93809950 - Magi1 NNNATCAAACTAAGACAAAA NGA 2 0.0374 Tier II
20 NC_000073.6 7 121743403 + Scnn1g NNNATCAAACTAAGAGACAC NGG 2 0.035 Tier II
21 NC_000076.6 10 69765637 - Ank3 NNNTTCAAACTAAGACACAA NGT 2 0.0103 Tier II
22 NC_000080.6 14 32236262 - Parg NNNATCAAACTAAGACAGAA NTG 2 0.0052 Tier II
23 NC_000082.6 16 34956863 - E130310I04Rik NNNAGAAAACTAAGACACAA NGG 2 0.5943 Tier III
24 NC_000072.6 6 126797706 + Gm29788 NNNAACAAACTAAGAAACAA NGG 2 0.5 Tier III
25 NC_000077.6 11 5771732 - Ube2d-ps NNNATGAAACTAAGACACAG NGG 2 0.3824 Tier III
26 NC_000067.6 1 31035152 + Gm28644 NNNCTCTAACTAAGACACAA NGG 2 0.1544 Tier III
27 NC_000067.6 1 31035152 + Gm29669 NNNCTCTAACTAAGACACAA NGG 2 0.1544 Tier III
28 NC_000082.6 16 95865220 - 1600002D24Rik NNNATCAAACGAAGACACCA NGG 2 0.0828 Tier III
29 NC_000074.6 8 107216757 - 4930517G24Rik NNNATCATACTAAGACAGAA NGG 2 0.0571 Tier III
30 NC_000077.6 11 56621906 - Gm32649 NNNATCAAACTAAGACAGAC NGG 2 0.0303 Tier III
31 NC_000069.6 3 19702228 - Gm42189 NNNATCAAACTAAGAGGCAA NGG 2 0.0271 Tier III
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Other clones with same target sequence:

(none)