Construct: sgRNA BRDN0001148548
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTAGCAAAACAGTGTACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BUB1 (699)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76771
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
110653500 |
- |
BUB1 |
NNNAGCAAAACAGTGTACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
15376278 |
+ |
AGMO |
NNNAGAAAAACAGTATACCC |
NGG |
2 |
0.8739 |
Tier II |
| 3 |
NC_000003.12 |
3 |
89200787 |
- |
EPHA3 |
NNNAGAAAAACAGAGTACCC |
NGG |
2 |
0.5748 |
Tier II |
| 4 |
NC_000003.12 |
3 |
76677179 |
+ |
ROBO2 |
NNNACCCAAACAGTGTACCC |
NGG |
2 |
0.3437 |
Tier II |
| 5 |
NC_000017.11 |
17 |
2929755 |
- |
RAP1GAP2 |
NNNTGGAAAACAGTGTACCC |
NGG |
2 |
0.3182 |
Tier II |
| 6 |
NC_000005.10 |
5 |
112822157 |
+ |
APC |
NNNAGCAAAAAATTGTACCC |
NGG |
2 |
0.225 |
Tier II |
| 7 |
NC_000013.11 |
13 |
23735820 |
- |
MIPEP |
NNNAGCATAACAGTGTGCCC |
NGG |
2 |
0.0756 |
Tier II |
| 8 |
NC_000001.11 |
1 |
69696221 |
+ |
LRRC7 |
NNNAGCACAACAGTGTTCCC |
NGG |
2 |
0.0571 |
Tier II |
| 9 |
NC_000002.12 |
2 |
25758549 |
- |
ASXL2 |
NNNAGGAAAACAGTGTACCC |
NGT |
2 |
0.0081 |
Tier II |
| 10 |
NC_000002.12 |
2 |
56296220 |
- |
CCDC85A |
NNNAGCAAAAAAGGGTACCC |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000019.10 |
19 |
39237834 |
- |
LOC124904717 |
NNNAGCTAAACAGTGTGCCC |
NGG |
2 |
0.0772 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
3615845 |
+ |
Tgs1 |
NNNAGTATAACAGTGTACCC |
NGG |
2 |
0.398 |
Tier II |
| 2 |
NC_000067.6 |
1 |
178331764 |
- |
Hnrnpu |
NNNAGCAAAACACTATACCC |
NGG |
2 |
0.3963 |
Tier II |
| 3 |
NC_000080.6 |
14 |
99875970 |
- |
Klf12 |
NNNAGGAAAACAGTGCACCC |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000080.6 |
14 |
31506900 |
+ |
Eaf1 |
NNNAGCAGACCAGTGTACCC |
NGG |
2 |
0.2444 |
Tier II |
| 5 |
NC_000073.6 |
7 |
96365278 |
- |
Tenm4 |
NNNAGCAATACAGTGGACCC |
NGG |
2 |
0.2077 |
Tier II |
| 6 |
NC_000070.6 |
4 |
33326846 |
- |
Rngtt |
NNNAGCACAACACTGTACCC |
NGG |
2 |
0.1805 |
Tier II |
| 7 |
NC_000076.6 |
10 |
41491784 |
+ |
Ppil6 |
NNNAGAAAAACAGTGTGCCC |
NGG |
2 |
0.1639 |
Tier II |
| 8 |
NC_000072.6 |
6 |
38910644 |
- |
Tbxas1 |
NNNACCAAAACAGTGTGCCC |
NGG |
2 |
0.1387 |
Tier II |
| 9 |
NC_000073.6 |
7 |
120857931 |
+ |
Eef2k |
NNNAACAAAACAGTGTTCCC |
NGG |
2 |
0.1156 |
Tier II |
| 10 |
NC_000067.6 |
1 |
55314429 |
+ |
Boll |
NNNAGCAAAACAGTGTCTCC |
NGG |
2 |
0.1134 |
Tier II |
| 11 |
NC_000076.6 |
10 |
10935327 |
+ |
Grm1 |
NNNAGCAAAACAGAGTCCCC |
NGG |
2 |
0.1092 |
Tier II |
| 12 |
NC_000070.6 |
4 |
9369838 |
- |
Clvs1 |
NNNAGCAAAACATTGTACCC |
NAG |
2 |
0.0778 |
Tier II |
| 13 |
NC_000073.6 |
7 |
116238698 |
+ |
Plekha7 |
NNNAGCAAAACAGTTTACCC |
NTG |
2 |
0.0056 |
Tier II |
| 14 |
NC_000077.6 |
11 |
121568262 |
+ |
Tbcd |
NNNAGCAAAATAGTGTACCC |
NGT |
2 |
0.005 |
Tier II |
| 15 |
NC_000073.6 |
7 |
120857931 |
+ |
Gm15774 |
NNNAACAAAACAGTGTTCCC |
NGG |
2 |
0.1156 |
Tier III |
| 16 |
NC_000077.6 |
11 |
19071159 |
- |
4933406G16Rik |
NNNAGCAAAACAGTGTCCTC |
NGG |
2 |
0.0814 |
Tier III |
| 17 |
NC_000073.6 |
7 |
128494067 |
+ |
Gm40457 |
NNNACCAAAACAGTGTACCC |
NGA |
2 |
0.0546 |
Tier III |
Other clones with same target sequence:
(none)
