Construct: sgRNA BRDN0001148548

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTAGCAAAACAGTGTACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BUB1 (699)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76771

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 110653500 - BUB1 NNNAGCAAAACAGTGTACCC NGG 0 1.0 Tier I
2 NC_000007.14 7 15376278 + AGMO NNNAGAAAAACAGTATACCC NGG 2 0.8739 Tier II
3 NC_000003.12 3 89200787 - EPHA3 NNNAGAAAAACAGAGTACCC NGG 2 0.5748 Tier II
4 NC_000003.12 3 76677179 + ROBO2 NNNACCCAAACAGTGTACCC NGG 2 0.3437 Tier II
5 NC_000017.11 17 2929755 - RAP1GAP2 NNNTGGAAAACAGTGTACCC NGG 2 0.3182 Tier II
6 NC_000005.10 5 112822157 + APC NNNAGCAAAAAATTGTACCC NGG 2 0.225 Tier II
7 NC_000013.11 13 23735820 - MIPEP NNNAGCATAACAGTGTGCCC NGG 2 0.0756 Tier II
8 NC_000001.11 1 69696221 + LRRC7 NNNAGCACAACAGTGTTCCC NGG 2 0.0571 Tier II
9 NC_000002.12 2 25758549 - ASXL2 NNNAGGAAAACAGTGTACCC NGT 2 0.0081 Tier II
10 NC_000002.12 2 56296220 - CCDC85A NNNAGCAAAAAAGGGTACCC NGG 2 0.0 Tier II
11 NC_000019.10 19 39237834 - LOC124904717 NNNAGCTAAACAGTGTGCCC NGG 2 0.0772 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 3615845 + Tgs1 NNNAGTATAACAGTGTACCC NGG 2 0.398 Tier II
2 NC_000067.6 1 178331764 - Hnrnpu NNNAGCAAAACACTATACCC NGG 2 0.3963 Tier II
3 NC_000080.6 14 99875970 - Klf12 NNNAGGAAAACAGTGCACCC NGG 2 0.3333 Tier II
4 NC_000080.6 14 31506900 + Eaf1 NNNAGCAGACCAGTGTACCC NGG 2 0.2444 Tier II
5 NC_000073.6 7 96365278 - Tenm4 NNNAGCAATACAGTGGACCC NGG 2 0.2077 Tier II
6 NC_000070.6 4 33326846 - Rngtt NNNAGCACAACACTGTACCC NGG 2 0.1805 Tier II
7 NC_000076.6 10 41491784 + Ppil6 NNNAGAAAAACAGTGTGCCC NGG 2 0.1639 Tier II
8 NC_000072.6 6 38910644 - Tbxas1 NNNACCAAAACAGTGTGCCC NGG 2 0.1387 Tier II
9 NC_000073.6 7 120857931 + Eef2k NNNAACAAAACAGTGTTCCC NGG 2 0.1156 Tier II
10 NC_000067.6 1 55314429 + Boll NNNAGCAAAACAGTGTCTCC NGG 2 0.1134 Tier II
11 NC_000076.6 10 10935327 + Grm1 NNNAGCAAAACAGAGTCCCC NGG 2 0.1092 Tier II
12 NC_000070.6 4 9369838 - Clvs1 NNNAGCAAAACATTGTACCC NAG 2 0.0778 Tier II
13 NC_000073.6 7 116238698 + Plekha7 NNNAGCAAAACAGTTTACCC NTG 2 0.0056 Tier II
14 NC_000077.6 11 121568262 + Tbcd NNNAGCAAAATAGTGTACCC NGT 2 0.005 Tier II
15 NC_000073.6 7 120857931 + Gm15774 NNNAACAAAACAGTGTTCCC NGG 2 0.1156 Tier III
16 NC_000077.6 11 19071159 - 4933406G16Rik NNNAGCAAAACAGTGTCCTC NGG 2 0.0814 Tier III
17 NC_000073.6 7 128494067 + Gm40457 NNNACCAAAACAGTGTACCC NGA 2 0.0546 Tier III
Download CSV

Other clones with same target sequence:

(none)

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