Construct: sgRNA BRDN0001148553
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGAAGAAAGGCCGTCTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK4 (6787)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77711
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
52763538 |
- |
NEK4 |
NNNAAGAAAGGCCGTCTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
133297945 |
- |
EXOC4 |
NNNAAGAAAGGTCGTATGTG |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000015.10 |
15 |
27512375 |
+ |
GABRG3 |
NNNAAGAAAGGCTGACTGTG |
NGG |
2 |
0.4053 |
Tier II |
| 4 |
NC_000017.11 |
17 |
1603289 |
- |
SLC43A2 |
NNNAAGAAAGGCCATCTCTG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000002.12 |
2 |
105389722 |
+ |
FHL2 |
NNNTTGAAAGGCCGTCTGTG |
NGG |
2 |
0.2314 |
Tier II |
| 6 |
NC_000022.11 |
22 |
40008950 |
- |
FAM83F |
NNNCAGAATGGCCGTCTGTG |
NGG |
2 |
0.2118 |
Tier II |
| 7 |
NC_000013.11 |
13 |
25802570 |
+ |
ATP8A2 |
NNNCAGAAAGGCAGTCTGTG |
NGG |
2 |
0.1357 |
Tier II |
| 8 |
NC_000016.10 |
16 |
29889533 |
- |
SEZ6L2 |
NNNAAGAAAGGCTGTGTGTG |
NGG |
2 |
0.1077 |
Tier II |
| 9 |
NC_000005.10 |
5 |
178223682 |
- |
PHYKPL |
NNNAAGAAAGGCAGCCTGTG |
NGG |
2 |
0.1049 |
Tier II |
| 10 |
NC_000003.12 |
3 |
73577712 |
- |
PDZRN3 |
NNNAAGAAAGGCAGTCTGTG |
NAG |
2 |
0.0997 |
Tier II |
| 11 |
NC_000012.12 |
12 |
105217230 |
- |
APPL2 |
NNNAAGAAAGGACGTCGGTG |
NGG |
2 |
0.084 |
Tier II |
| 12 |
NC_000005.10 |
5 |
77517944 |
- |
WDR41 |
NNNAAGAAAGGCCCTCTGTG |
NTG |
2 |
0.0167 |
Tier II |
| 13 |
NC_000010.11 |
10 |
79298748 |
- |
ZMIZ1 |
NNNAAGAAAGGCTGTCTGTG |
NGC |
2 |
0.0156 |
Tier II |
| 14 |
NC_000021.9 |
21 |
17211074 |
- |
NEK4P1 |
NNNAAGAAAGACCCTCTGTG |
NGG |
2 |
0.4286 |
Tier III |
| 15 |
NC_000021.9 |
21 |
17211074 |
- |
LOC107985511 |
NNNAAGAAAGACCCTCTGTG |
NGG |
2 |
0.4286 |
Tier III |
| 16 |
NC_000001.11 |
1 |
189181623 |
- |
LOC105371657 |
NNNAAGAAAGGCAGTGTGTG |
NGG |
2 |
0.0592 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
82131011 |
- |
Ccdc134 |
NNNAACAAAGGCCATCTGTG |
NGG |
2 |
0.5114 |
Tier II |
| 2 |
NC_000074.6 |
8 |
70775460 |
- |
Pik3r2 |
NNNAAGAAAGGCTGTCAGTG |
NGG |
2 |
0.3733 |
Tier II |
| 3 |
NC_000074.6 |
8 |
121967327 |
+ |
Banp |
NNNAAGCAAGGCCATCTGTG |
NGG |
2 |
0.3281 |
Tier II |
| 4 |
NC_000085.6 |
19 |
40654753 |
+ |
Entpd1 |
NNNCAGAAAGGCCATCTGTG |
NGG |
2 |
0.2647 |
Tier II |
| 5 |
NC_000078.6 |
12 |
75840493 |
+ |
Syne2 |
NNNAACAAAGTCCGTCTGTG |
NGG |
2 |
0.2622 |
Tier II |
| 6 |
NC_000077.6 |
11 |
86941983 |
- |
Ypel2 |
NNNAAGAAAGGCCTTCTGTA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000072.6 |
6 |
86468521 |
+ |
C87436 |
NNNAAGAAAGGCCGCCTGTT |
NGG |
2 |
0.1909 |
Tier II |
| 8 |
NC_000075.6 |
9 |
21394449 |
+ |
Ilf3 |
NNNAAGAAGGGCCTTCTGTG |
NGG |
2 |
0.1778 |
Tier II |
| 9 |
NC_000075.6 |
9 |
21181884 |
+ |
Pde4a |
NNNCAGCAAGGCCGTCTGTG |
NGG |
2 |
0.1544 |
Tier II |
| 10 |
NC_000083.6 |
17 |
28411355 |
+ |
Fkbp5 |
NNNAAGAAAGGCGGTCTCTG |
NGG |
2 |
0.0649 |
Tier II |
| 11 |
NC_000076.6 |
10 |
59861449 |
- |
Micu1 |
NNNAAGCAAGGCCGGCTGTG |
NGG |
2 |
0.0219 |
Tier II |
| 12 |
NC_000077.6 |
11 |
75899766 |
+ |
Rph3al |
NNNAAGAAAGGCTGTCTGTG |
NGC |
2 |
0.0156 |
Tier II |
| 13 |
NC_000067.6 |
1 |
155199644 |
+ |
Stx6 |
NNNAACAAAGGCCGTCTGTG |
NGT |
2 |
0.011 |
Tier II |
| 14 |
NC_000073.6 |
7 |
81850351 |
+ |
Gm51491 |
NNNAGGAAAGGCTGTCTGTG |
NGG |
2 |
0.504 |
Tier III |
| 15 |
NC_000074.6 |
8 |
121852736 |
+ |
Gm26812 |
NNNAAGGAAGGGCGTCTGTG |
NGG |
2 |
0.3137 |
Tier III |
| 16 |
NC_000070.6 |
4 |
150837082 |
- |
1700045H11Rik |
NNNAAGCATGGCCGTCTGTG |
NGG |
2 |
0.2625 |
Tier III |
| 17 |
NC_000069.6 |
3 |
143747494 |
- |
Gm33867 |
NNNAAGAAAGGCCTTCTATG |
NGG |
2 |
0.1846 |
Tier III |
| 18 |
NC_000074.6 |
8 |
79401727 |
- |
Gm51578 |
NNNAAGAAAGGCCATCTGTG |
NTG |
2 |
0.0292 |
Tier III |
Other clones with same target sequence:
(none)
