Construct: sgRNA BRDN0001148565

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAAGACAGACCAACCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK2 (9344)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77249

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 29981924 + TAOK2 NNNAGACAGACCAACCTCAG NGG 0 1.0 Tier I
2 NC_000005.10 5 122008244 - SRFBP1 NNNAGACAGGCCAACATCAG NGG 2 0.5556 Tier II
3 NC_000012.12 12 29172480 - FAR2 NNNAGACAAACAAACCTCAG NGG 2 0.4592 Tier II
4 NC_000023.11 X 18922523 + PHKA2 NNNAGAGGGACCAACCTCAG NGG 2 0.3451 Tier II
5 NC_000009.12 9 37188457 - ZCCHC7 NNNGGAGAGACCAACCTCAG NGG 2 0.2941 Tier II
6 NC_000012.12 12 6203510 + CD9 NNNAGAGAGGCCAACCTCAG NGG 2 0.2614 Tier II
7 NC_000010.11 10 51237497 + PRKG1 NNNAGATAGACCAACTTCAG NGG 2 0.25 Tier II
8 NC_000005.10 5 135585533 - SLC25A48 NNNACACAGAGCAACCTCAG NGG 2 0.1964 Tier II
9 NC_000011.10 11 113177812 - NCAM1 NNNAGACAGTCCCACCTCAG NGG 2 0.1858 Tier II
10 NC_000003.12 3 120709481 + RABL3 NNNAGACAGAGCAACCCCAG NGG 2 0.1765 Tier II
11 NC_000018.10 18 23218779 + CABLES1 NNNAGACAGACCAACCTTAG NAG 2 0.1667 Tier II
12 NC_000001.11 1 148587430 + NBPF14 NNNAGACACACCTACCTCAG NGG 2 0.1615 Tier II
13 NC_000016.10 16 2028040 - NHERF2 NNNAGACAGACACACCTCAG NGG 2 0.1504 Tier II
14 NC_000014.9 14 72259055 + RGS6 NNNAGAGAGACCACCCTCAG NGG 2 0.1008 Tier II
15 NC_000001.11 1 156168731 - SEMA4A NNNAGCCAGACCACCCTCAG NGG 2 0.0974 Tier II
16 NC_000002.12 2 136958960 + THSD7B NNNAGACAGACGCACCTCAG NGG 2 0.0936 Tier II
17 NC_000019.10 19 6228843 + MLLT1 NNNAGACAGCCCACCCTCAG NGG 2 0.0714 Tier II
18 NC_000016.10 16 3448911 - NAA60 NNNACACAGACCAATCTCAG NGG 2 0.0524 Tier II
19 NC_000007.14 7 1101203 - C7orf50 NNNAGACAGACCAACCGCAG NAG 2 0.0305 Tier II
20 NC_000012.12 12 5781108 + ANO2 NNNAGACAGCCCAAGCTCAG NGG 2 0.0167 Tier II
21 NC_000013.11 13 110537576 + RAB20 NNNAAACAGACCAACCTCAG NGT 2 0.014 Tier II
22 NC_000012.12 12 49912426 + LINC02395 NNNAGACAGACCATCCCCAG NGG 2 0.3765 Tier III
23 NC_000011.10 11 30851143 - LOC107984419 NNNAGACAGACCAGCCTCAC NGG 2 0.2 Tier III
24 NC_000005.10 5 172760483 + LOC101928093 NNNAGACAGATCACCCTCAG NGG 2 0.0659 Tier III
25 NC_000003.12 3 110457918 - LOC105374036 NNNAGACAGACCAAACTCAG NTG 2 0.0087 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 126875870 - Taok2 NNNAGACAGACCAACCTCAG NGG 0 1.0 Tier I
2 NC_000069.6 3 157281370 - Negr1 NNNACACAGACCAACCTCAG NGG 1 0.7857 Tier II
3 NC_000067.6 1 58045988 - Aox1 NNNAGACAGAACAACCCCAG NGG 2 0.5294 Tier II
4 NC_000070.6 4 125650306 - Grik3 NNNAGACAGACCAGCCTCAG NGG 1 0.4667 Tier II
5 NC_000067.6 1 39970064 - Map4k4 NNNAGACAGACAGACCTCAG NGG 2 0.4658 Tier II
6 NC_000072.6 6 51561836 - Snx10 NNNAGACAGACAGACCTCAG NGG 2 0.4658 Tier II
7 NC_000079.6 13 57768042 + Spock1 NNNGGGCAGACCAACCTCAG NGG 2 0.4464 Tier II
8 NC_000072.6 6 127617647 + Cracr2a NNNAGAGAGACCAACCTCAA NGG 2 0.4412 Tier II
9 NC_000074.6 8 84701453 + Lyl1 NNNAGCTAGACCAACCTCAG NGG 2 0.3693 Tier II
10 NC_000067.6 1 167263266 + Uck2 NNNAGACAGACTGACCTCAG NGG 2 0.3512 Tier II
11 NC_000085.6 19 53771316 - Rbm20 NNNTGACAGACCAACCACAG NGG 2 0.3394 Tier II
12 NC_000077.6 11 118775877 + Rbfox3 NNNAGACAGACTAACCTCTG NGG 2 0.2899 Tier II
13 NC_000068.7 2 26428077 - Sec16a NNNAGAGAGACCAACCTCTG NGG 2 0.2534 Tier II
14 NC_000075.6 9 90210473 + Tbc1d2b NNNAGACAGACCAGCCACAG NGG 2 0.2489 Tier II
15 NC_000072.6 6 72515080 + Sh2d6 NNNAGACAGAACTACCTCAG NGG 2 0.225 Tier II
16 NC_000075.6 9 86307981 + Ube2cbp NNNACACAGACCAACCTCAG NAG 2 0.2037 Tier II
17 NC_000077.6 11 6401894 + Zmiz2 NNNAGACAGACCTACCACAG NGG 2 0.16 Tier II
18 NC_000084.6 18 7262802 - Armc4 NNNAGACAGGCCAACCTCAG NAG 2 0.144 Tier II
19 NC_000068.7 2 57337187 - Gpd2 NNNAGACAGACCAACCTAAG NAG 2 0.1396 Tier II
20 NC_000075.6 9 27086232 - Ncapd3 NNNAGAGAGAGCAACCTCAG NGG 2 0.1176 Tier II
21 NC_000085.6 19 5770890 + Scyl1 NNNAGACAGAGCAACCTGAG NGG 2 0.0333 Tier II
22 NC_000072.6 6 97963918 - Mitf NNNAGACAGACCAAGCTCAC NGG 2 0.0214 Tier II
23 NC_000070.6 4 151476595 - Camta1 NNNAGACAGACCAGCCTCAG NTG 2 0.0182 Tier II
24 NC_000071.6 5 36951286 - Ppp2r2c NNNAGACAGACCAAGTTCAG NGG 2 0.0154 Tier II
25 NC_000076.6 10 63455435 - Ctnna3 NNNAGACAGACCAAGCTCAG NAG 2 0.013 Tier II
26 NC_000084.6 18 31997516 + Myo7b NNNACACAGACCAACCTCAG NGT 2 0.0127 Tier II
27 NC_000078.6 12 74114120 + Syt16 NNNAGACATACCAACCTCAG NGT 2 0.0086 Tier II
28 NC_000076.6 10 43607834 - F930017D23Rik NNNAGACAGACAGACCTCAG NGG 2 0.4658 Tier III
29 NC_000084.6 18 67795736 + 4930549G23Rik NNNAGCCAGAACAACCTCAG NGG 2 0.3409 Tier III
30 NC_000085.6 19 53771316 - Gm30890 NNNTGACAGACCAACCACAG NGG 2 0.3394 Tier III
31 NC_000067.6 1 43718232 - Gm30634 NNNAGCCAGACAAACCTCAG NGG 2 0.3247 Tier III
32 NC_000067.6 1 164464370 + Gm32391 NNNAGACTGACCAACCCCAG NGG 2 0.3025 Tier III
33 NC_000076.6 10 42740117 - Gm15199 NNNAGACAGACCAACCCCAG NAG 2 0.183 Tier III
34 NC_000082.6 16 31488551 + Gm34032 NNNAGACAGACCTACCTAAG NGG 2 0.1615 Tier III
35 NC_000084.6 18 38089102 + Gm30093 NNNAGACAGACCGACCTCAG NGA 2 0.0453 Tier III
36 NC_000072.6 6 49634064 - Gm31226 NNNAGACAGACCAACCCCAG NGC 2 0.0157 Tier III
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Other clones with same target sequence:

(none)