Construct: sgRNA BRDN0001148577
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAACCTACCTCCTATCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK2 (5610)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75638
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
37147696 |
+ |
EIF2AK2 |
NNNACCTACCTCCTATCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
14698899 |
- |
FAM107B |
NNNACCTACCTCCTATTATT |
NGG |
2 |
0.3267 |
Tier II |
| 3 |
NC_000002.12 |
2 |
149412032 |
+ |
LYPD6 |
NNNACCTACCTCCTATTATC |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000010.11 |
10 |
15817434 |
- |
MINDY3 |
NNNACCTACCTTCTATCATG |
NAG |
2 |
0.1396 |
Tier II |
| 5 |
NC_000007.14 |
7 |
40993615 |
- |
SUGCT |
NNNACCTACCTCCTATCAAG |
NAG |
2 |
0.0741 |
Tier II |
| 6 |
NC_000003.12 |
3 |
133467846 |
- |
BFSP2 |
NNNACCTACATCCTATCATG |
NGC |
2 |
0.0193 |
Tier II |
| 7 |
NC_000003.12 |
3 |
13702962 |
- |
LINC00620 |
NNNACCAACCTCCTAACATG |
NGG |
2 |
0.7955 |
Tier III |
| 8 |
NC_000010.11 |
10 |
15817434 |
- |
LOC124902383 |
NNNACCTACCTTCTATCATG |
NAG |
2 |
0.1396 |
Tier III |
| 9 |
NC_000003.12 |
3 |
133467846 |
- |
BFSP2-AS1 |
NNNACCTACATCCTATCATG |
NGC |
2 |
0.0193 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
34659815 |
- |
Ifit1bl1 |
NNNACCTACATTCTATCATG |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000081.6 |
15 |
81335366 |
+ |
Slc25a17 |
NNNTCCTACCTCCTGTCATG |
NGG |
2 |
0.4136 |
Tier II |
| 3 |
NC_000078.6 |
12 |
53540416 |
+ |
Npas3 |
NNNACCTACCTAATATCATG |
NGG |
2 |
0.2747 |
Tier II |
| 4 |
NC_000073.6 |
7 |
102094871 |
+ |
Trpc2 |
NNNACCTACCTCCTATCATC |
NGC |
2 |
0.0095 |
Tier II |
| 5 |
NC_000073.6 |
7 |
102094871 |
+ |
Xntrpc |
NNNACCTACCTCCTATCATC |
NGC |
2 |
0.0095 |
Tier II |
Other clones with same target sequence:
(none)
