Construct: sgRNA BRDN0001148598

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAACTTCTCAACATTGCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK3 (79646)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76294

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 168568705 - PANK3 NNNCTTCTCAACATTGCAGA NGG 0 1.0 Tier I
2 NC_000010.11 10 28591894 - WAC NNNCTTCTCAAAATTGCAAA NGG 2 0.5102 Tier II
3 NC_000003.12 3 116415371 + LSAMP NNNCTTCACAACATTGCTGA NGG 2 0.4 Tier II
4 NC_000008.11 8 101940141 - NCALD NNNCTTCTCAAGATTGCAAA NGG 2 0.3175 Tier II
5 NC_000017.11 17 33200525 + ASIC2 NNNCTTCTCTTCATTGCAGA NGG 2 0.2715 Tier II
6 NC_000001.11 1 44440278 + RNF220 NNNCTTTTCATCATTGCAGA NGG 2 0.25 Tier II
7 NC_000001.11 1 97315043 - DPYD NNNCTCCTCAACATTGCAGA NAG 2 0.2357 Tier II
8 NC_000001.11 1 31701039 + COL16A1 NNNCTTCTCATCAATGCAGA NGG 2 0.1905 Tier II
9 NC_000011.10 11 75962333 + UVRAG NNNCTTCTCAACTTAGCAGA NGG 2 0.1737 Tier II
10 NC_000007.14 7 48377921 - ABCA13 NNNCATCTCAACATTGCAGA NAG 2 0.1296 Tier II
11 NC_000003.12 3 79339226 + ROBO1 NNNCTTCTCAACATAGCAGA NCG 2 0.062 Tier II
12 NC_000017.11 17 69504236 - MAP2K6 NNNCTTCTCTACATTGCAGA NGA 2 0.0613 Tier II
13 NC_000001.11 1 62617416 - DOCK7 NNNCTTCTCAACATTGCAGC NAG 2 0.0589 Tier II
14 NC_000016.10 16 84866886 - CRISPLD2 NNNCTTCTTAACATTGGAGA NGG 2 0.0515 Tier II
15 NC_000009.12 9 20290328 - SLC24A2 NNNCTCCTCAACATGGCAGA NGG 2 0.0455 Tier II
16 NC_000016.10 16 28571190 - SGF29 NNNATTCTCAACATGGCAGA NGG 2 0.0421 Tier II
17 NC_000005.10 5 38375783 - EGFLAM NNNCTTCTCAACATTGCAGT NGA 2 0.0417 Tier II
18 NC_000008.11 8 62689079 - NKAIN3 NNNCTTCTCAACATTGAAGA NGA 2 0.0324 Tier II
19 NC_000009.12 9 70124522 - MAMDC2 NNNCATCTCAACATGGCAGA NGG 2 0.025 Tier II
20 NC_000015.10 15 28286885 - HERC2 NNNCTTCTCAACATTGCAGC NTG 2 0.0089 Tier II
21 NC_000015.10 15 28624541 + LOC124903450 NNNCTTCTCAACATTGCAGC NTG 2 0.0089 Tier II
22 NC_000008.11 8 6817303 + XKR5 NNNCTTCTCAACAGAGCAGA NGG 2 0.0 Tier II
23 NC_000018.10 18 7772039 - PTPRM NNNCTTCTCAACAGTGCAGA NAG 2 0.0 Tier II
24 NC_000001.11 1 242241721 - PLD5 NNNCTTCTCCACAGTGCAGA NGG 2 0.0 Tier II
25 NC_000004.12 4 155367961 - MAP9 NNNCTTCTGAACATTTCAGA NGG 2 0.0 Tier II
26 NC_000017.11 17 66667916 - PRKCA NNNCTTCTTAACATTTCAGA NGG 2 0.0 Tier II
27 NC_000007.14 7 118549509 - LOC105375473 NNNCTTCTTTACATTGCAGA NGG 2 0.7721 Tier III
28 NC_000011.10 11 101135786 - PGR-AS1 NNNCTAATCAACATTGCAGA NGG 2 0.65 Tier III
29 NC_000011.10 11 50268483 + LOC646801 NNNCTTCTCAATATTGCAAA NGG 2 0.3846 Tier III
30 NC_000010.11 10 57829697 - LOC105378314 NNNCTTCTCAAGGTTGCAGA NGG 2 0.2899 Tier III
31 NC_000008.11 8 78829469 + MITA1 NNNCTTCTCAACATTGCACT NGG 2 0.269 Tier III
32 NC_000001.11 1 97315043 - DPYD-AS1 NNNCTCCTCAACATTGCAGA NAG 2 0.2357 Tier III
33 NC_000008.11 8 20717127 + LOC105379315 NNNTTTCTCAACATTGCAGC NGG 2 0.1818 Tier III
34 NC_000007.14 7 118505965 - LOC105375473 NNNCTTCTCAACATGGCAGA NGG 1 0.05 Tier III
35 NC_000004.12 4 31719512 - LOC105374566 NNNCTTCACAACATGGCAGA NGG 2 0.04 Tier III
36 NC_000018.10 18 5917355 - LOC121725015 NNNCTTATCAACATGGCAGA NGG 2 0.0375 Tier III
37 NC_000006.12 6 152685856 - LOC105378061 NNNCATCTCAACATGGCAGA NGG 2 0.025 Tier III
38 NC_000007.14 7 142506865 - TRB NNNCTTCTCAACATTGCAGG NGT 2 0.0123 Tier III
39 NC_000015.10 15 20471038 - HERC2P3 NNNCTTCTCAACATTGCAGC NTG 2 0.0089 Tier III
40 NC_000015.10 15 22529365 + HERC2P2 NNNCTTCTCAACATTGCAGC NTG 2 0.0089 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 24920084 - Adam32 NNNATTCTTAACATTGCAGA NGG 2 0.7368 Tier I
2 NC_000069.6 3 93219996 - Flg2 NNNCTTCTCAACCTTCCAGA NGG 2 0.0 Tier I
3 NC_000067.6 1 66372558 + Map2 NNNCTTCCCAACATTGCATA NGG 2 0.6667 Tier II
4 NC_000077.6 11 57176405 + Gria1 NNNCTTCCCACCATTGCAGA NGG 2 0.4 Tier II
5 NC_000070.6 4 121162995 + Rlf NNNATTCTCAACATTGAAGA NGG 2 0.393 Tier II
6 NC_000083.6 17 75545974 + Fam98a NNNCTTCTCAGTATTGCAGA NGG 2 0.35 Tier II
7 NC_000086.7 X 9660349 - Dynlt3 NNNCAGCTCAACATTGCAGA NGG 2 0.2857 Tier II
8 NC_000086.7 X 120847597 - Pcdh11x NNNCTTGTCAACATAGCAGA NGG 2 0.2724 Tier II
9 NC_000075.6 9 71626733 + Cgnl1 NNNCTTCTCAACATTGCACT NGG 2 0.269 Tier II
10 NC_000068.7 2 35691113 - Dab2ip NNNATTCTCAACACTGCAGA NGG 2 0.2406 Tier II
11 NC_000071.6 5 103745604 - Aff1 NNNCTTCCCAACATTGCAGC NGG 2 0.2273 Tier II
12 NC_000071.6 5 118663926 - Med13l NNNCTTCTCGCCATTGCAGA NGG 2 0.2222 Tier II
13 NC_000082.6 16 11148224 + Zc3h7a NNNCTTCTCAAAACTGCAGA NGG 2 0.2041 Tier II
14 NC_000068.7 2 63098702 - Kcnh7 NNNCTTCTCAACTATGCAGA NGG 2 0.1857 Tier II
15 NC_000079.6 13 68782186 - Adcy2 NNNCTTCTCAACATTGCAGA NGA 1 0.0694 Tier II
16 NC_000079.6 13 74291694 + Ahrr NNNCTTCTCAAAATTGGAGA NGG 2 0.042 Tier II
17 NC_000084.6 18 69579915 + Tcf4 NNNCTTCTCAACAATGGAGA NGG 2 0.0364 Tier II
18 NC_000084.6 18 16680885 - Cdh2 NNNCTTCTCAACACGGCAGA NGG 2 0.0143 Tier II
19 NC_000078.6 12 85287620 - Acyp1 NNNCTTCTCCACATTGCAGA NGT 2 0.0054 Tier II
20 NC_000078.6 12 85287620 - Zc2hc1c NNNCTTCTCCACATTGCAGA NGT 2 0.0054 Tier II
21 NC_000082.6 16 97427153 - Bace2 NNNCTTCACAACATTTCAGA NGG 2 0.0 Tier II
22 NC_000074.6 8 67916229 - Psd3 NNNCTTCTCAACATTTCAGA NTG 2 0.0 Tier II
23 NC_000072.6 6 28799219 + Snd1 NNNCTTCTCTACATTTCAGA NGG 2 0.0 Tier II
24 NC_000071.6 5 3311397 - Gm36470 NNNCTTCTCAACATAGCAGA NAG 2 0.1501 Tier III
25 NC_000072.6 6 70079467 + Igk NNNCTTCTCAACATTGAAGA NGA 2 0.0324 Tier III
26 NC_000079.6 13 69004714 - Gm35161 NNNCTTCTCAACATTTCAGA NGA 2 0.0 Tier III
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Other clones with same target sequence:

(none)