Construct: sgRNA BRDN0001148604

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACACTCCAGAGATCCACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK6 (1021)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75646

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 92671475 + CDK6 NNNACTCCAGAGATCCACGG NGG 0 1.0 Tier I
2 NC_000007.14 7 44112565 + AEBP1 NNNACTCCAGGTATCCACGG NGG 2 0.25 Tier I
3 NC_000005.10 5 154758560 - LARP1 NNNACTTCAGAGATCCACGT NGG 2 0.5688 Tier II
4 NC_000011.10 11 62447759 - AHNAK NNNACTTCAGAGGTCCACGG NGG 2 0.5299 Tier II
5 NC_000005.10 5 179061768 + ZNF354C NNNACTCCAGACATCCATGG NGG 2 0.3403 Tier II
6 NC_000002.12 2 236035742 - AGAP1 NNNACTCCACAGATCCACGT NGG 2 0.28 Tier II
7 NC_000002.12 2 98599635 + COA5 NNNACTCCAGTGAACCACGG NGG 2 0.1905 Tier II
8 NC_000020.11 20 10254344 + SNAP25 NNNACTCCAGAGCTCCATGG NGG 2 0.1353 Tier II
9 NC_000004.12 4 30977798 - PCDH7 NNNACTCCAGAGATCCTCAG NGG 2 0.0952 Tier II
10 NC_000009.12 9 124794167 - OLFML2A NNNACTCCAGAGATCCTCTG NGG 2 0.0889 Tier II
11 NC_000003.12 3 54309573 - CACNA2D3 NNNATTCCAGAGATCCAGGG NGG 2 0.0848 Tier II
12 NC_000007.14 7 144524505 - TPK1 NNNACTCCAGAGATCCAGGG NGA 2 0.0093 Tier II
13 NC_000005.10 5 157988077 + MARK2P11 NNNACTCCTGAGATCCATGG NGG 2 0.3857 Tier III
14 NC_000002.12 2 234830992 - LOC101927896 NNNTCTCCAGAGATCCACCG NGG 2 0.2853 Tier III
15 NC_000014.9 14 36288476 + LOC107984003 NNNACTCCAGACATCCAAGG NGG 2 0.2851 Tier III
16 NC_000006.12 6 129882974 - LOC105377999 NNNTCTCCAGTGATCCACGG NGG 2 0.1958 Tier III
17 NC_000005.10 5 176192823 + CEP192P1 NNNACACCAGAGCTCCACGG NGG 2 0.1825 Tier III
18 NC_000005.10 5 15384444 - MARK2P5 NNNACTCCAGAGATCCCCAG NGG 2 0.1261 Tier III
19 NC_000005.10 5 177946094 - LOC100128340 NNNACGCCAGAGCTCCACGG NGG 2 0.1203 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 3473180 - Cdk6 NNNACTCCAGAGGTCCACAG NGG 2 0.4658 Tier I
2 NC_000068.7 2 72005427 - Rapgef4 NNNACGCCAGAAATCCACGG NGG 2 0.5333 Tier II
3 NC_000080.6 14 28504931 - Wnt5a NNNACTCCAGAGGTCCATGG NGG 2 0.4193 Tier II
4 NC_000080.6 14 22059302 - Lrmda NNNACTCCTGAGATCCACTG NGG 2 0.4 Tier II
5 NC_000073.6 7 44342965 + Shank1 NNNACTCCAGAGAACCATGG NGG 2 0.398 Tier II
6 NC_000083.6 17 51086911 + Tbc1d5 NNNACTGCAGAGATCCACGT NGG 2 0.3294 Tier II
7 NC_000083.6 17 67931513 + Arhgap28 NNNACTCCAGAGTTCCACAG NGG 2 0.2143 Tier II
8 NC_000074.6 8 94977787 + Adgrg1 NNNCCTCCTGAGATCCACGG NGG 2 0.2118 Tier II
9 NC_000078.6 12 88453359 - Adck1 NNNACTCCAGAGATCTACCG NGG 2 0.1379 Tier II
10 NC_000074.6 8 10599984 + Myo16 NNNACTCCAGAGATCAAGGG NGG 2 0.1333 Tier II
11 NC_000077.6 11 102014183 + Mpp3 NNNACTCCAGAGGTCCTCGG NGG 2 0.087 Tier II
12 NC_000082.6 16 96887779 - Dscam NNNACTCCAGAGATCCACTG NGT 2 0.0108 Tier II
13 NC_000067.6 1 36699686 + Actr1b NNNACTCCAGAGATCCAGGG NGT 2 0.0022 Tier II
14 NC_000067.6 1 62748139 + Nrp2 NNNACTCCAGGGAGCCACGG NGG 2 0.0 Tier II
15 NC_000073.6 7 38491907 + Gm6819 NNNACTCCAGAGATCAACAG NGG 2 0.7143 Tier III
16 NC_000080.6 14 22059302 - Gm41107 NNNACTCCTGAGATCCACTG NGG 2 0.4 Tier III
17 NC_000082.6 16 96887779 - Gm32432 NNNACTCCAGAGATCCACTG NGT 2 0.0108 Tier III
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Other clones with same target sequence:

(none)