Construct: sgRNA BRDN0001148610
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGGCCACCTCACAAAGTAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP4K4 (9448)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000002.12 | 2 | 101869683 | + | MAP4K4 | NNNGCCACCTCACAAAGTAA | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 133628444 | - | TOPBP1 | NNNGCCACTTCACAAAGTAG | NGG | 2 | 0.6691 | Tier I |
3 | NC_000008.11 | 8 | 112849510 | + | CSMD3 | NNNGCAACCTAACAAAGTAA | NGG | 2 | 0.6964 | Tier II |
4 | NC_000016.10 | 16 | 88299935 | - | ZNF469 | NNNGCCACCACACAAAGAAA | NGG | 2 | 0.5714 | Tier II |
5 | NC_000015.10 | 15 | 55278390 | + | RAB27A | NNNGCCACCTCACAAAGAAG | NGG | 2 | 0.5098 | Tier II |
6 | NC_000012.12 | 12 | 118037229 | + | RFC5 | NNNGCCACCCCACAAAGTAG | NGG | 2 | 0.4078 | Tier II |
7 | NC_000012.12 | 12 | 118037229 | + | WSB2 | NNNGCCACCCCACAAAGTAG | NGG | 2 | 0.4078 | Tier II |
8 | NC_000002.12 | 2 | 216687407 | + | IGFBP5 | NNNGACACCTCACAGAGTAA | NGG | 2 | 0.3714 | Tier II |
9 | NC_000008.11 | 8 | 56960830 | - | BPNT2 | NNNGACTCCTCACAAAGTAA | NGG | 2 | 0.25 | Tier II |
10 | NC_000008.11 | 8 | 10344765 | - | MSRA | NNNACCACCTCACACAGTAA | NGG | 2 | 0.2455 | Tier II |
11 | NC_000003.12 | 3 | 54971914 | + | CACNA2D3 | NNNTCCACCTCACAGAGTAA | NGG | 2 | 0.2364 | Tier II |
12 | NC_000013.11 | 13 | 92778602 | + | GPC5 | NNNGCCACCTCACTAAGGAA | NGG | 2 | 0.1778 | Tier II |
13 | NC_000016.10 | 16 | 4430322 | - | DNAJA3 | NNNCCCACCTCCCAAAGTAA | NGG | 2 | 0.1393 | Tier II |
14 | NC_000012.12 | 12 | 112307173 | - | HECTD4 | NNNGCCCCCTCCCAAAGTAA | NGG | 2 | 0.1151 | Tier II |
15 | NC_000022.11 | 22 | 43057672 | + | TTLL1 | NNNGCCACCTCAAAAAGTAA | NAG | 2 | 0.0997 | Tier II |
16 | NC_000009.12 | 9 | 14499392 | - | NFIB | NNNGCCACCTCACACAGGAA | NGG | 2 | 0.0909 | Tier II |
17 | NC_000015.10 | 15 | 29287466 | - | ENTREP2 | NNNGCCACCTCAAAAGGTAA | NGG | 2 | 0.074 | Tier II |
18 | NC_000005.10 | 5 | 95108408 | + | MCTP1 | NNNGCAACCTCACAAAGTAA | NGA | 2 | 0.0645 | Tier II |
19 | NC_000006.12 | 6 | 77472027 | + | LOC105377864 | NNNGCAACCTCACAAAGTAA | NGA | 2 | 0.0645 | Tier II |
20 | NC_000018.10 | 18 | 55476301 | + | TCF4 | NNNGCCACCTCACAAAGTGA | NGA | 2 | 0.026 | Tier II |
21 | NC_000021.9 | 21 | 37238189 | - | VPS26C | NNNGCCACCTCACATTGTAA | NGG | 2 | 0.0 | Tier II |
22 | NC_000006.12 | 6 | 81764378 | + | LOC105377873 | NNNGCCACATCACAAAGAAA | NGG | 2 | 0.5714 | Tier III |
23 | NC_000002.12 | 2 | 216687407 | + | LOC124900515 | NNNGACACCTCACAGAGTAA | NGG | 2 | 0.3714 | Tier III |
24 | NC_000016.10 | 16 | 53370118 | + | MPHOSPH10P1 | NNNTCCAGCTCACAAAGTAA | NGG | 2 | 0.2338 | Tier III |
25 | NC_000011.10 | 11 | 115937614 | + | LINC02703 | NNNGCCAACTCACACAGTAA | NGG | 2 | 0.1773 | Tier III |
26 | NC_000007.14 | 7 | 72866971 | - | SPDYE7P | NNNTCCACCTCTCAAAGTAA | NGG | 2 | 0.1212 | Tier III |
27 | NC_000023.11 | X | 20674761 | + | LOC124905257 | NNNGCCACCTCAGAAAGTTA | NGG | 2 | 0.0734 | Tier III |
28 | NC_000018.10 | 18 | 55476301 | + | TCF4-AS1 | NNNGCCACCTCACAAAGTGA | NGA | 2 | 0.026 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 40009749 | + | Map4k4 | NNNGCCACCTCACAAAGTAA | NAG | 1 | 0.2593 | Tier I |
2 | NC_000082.6 | 16 | 75876587 | + | Samsn1 | NNNTCCACCTGACAAAGTAA | NGG | 2 | 0.0909 | Tier I |
3 | NC_000076.6 | 10 | 69731103 | + | Ank3 | NNNGCCACCTCACAAAGTAG | NGG | 1 | 0.7647 | Tier II |
4 | NC_000070.6 | 4 | 97623859 | - | Nfia | NNNGCCACCTCACAAAGTTG | NGG | 2 | 0.4118 | Tier II |
5 | NC_000084.6 | 18 | 21027311 | + | Rnf138 | NNNGCCAAGTCACAAAGTAA | NGG | 2 | 0.4024 | Tier II |
6 | NC_000081.6 | 15 | 38049956 | + | Ubr5 | NNNGACAGCTCACAAAGTAA | NGG | 2 | 0.3673 | Tier II |
7 | NC_000071.6 | 5 | 32065372 | - | Babam2 | NNNGCAACCTCACAAAGTGA | NGG | 2 | 0.3482 | Tier II |
8 | NC_000073.6 | 7 | 143209563 | + | Kcnq1 | NNNGCCAGCTCACTAAGTAA | NGG | 2 | 0.3429 | Tier II |
9 | NC_000075.6 | 9 | 41129797 | + | Ubash3b | NNNGCCATCTCACAAAGTGA | NGG | 2 | 0.3281 | Tier II |
10 | NC_000068.7 | 2 | 109230325 | - | Mettl15 | NNNGCTACCTCACAAATTAA | NGG | 2 | 0.2321 | Tier II |
11 | NC_000067.6 | 1 | 11302676 | + | Prex2 | NNNGCCACATCACAAAGTAA | NAG | 2 | 0.2222 | Tier II |
12 | NC_000077.6 | 11 | 4946939 | - | Nefh | NNNGCCACCTGACAAAGTAG | NGG | 2 | 0.1912 | Tier II |
13 | NC_000079.6 | 13 | 93703172 | + | Dmgdh | NNNGGCACCTCACAAACTAA | NGG | 2 | 0.1412 | Tier II |
14 | NC_000072.6 | 6 | 106669843 | - | Cntn4 | NNNGCCACCTCCCAAATTAA | NGG | 2 | 0.0658 | Tier II |
15 | NC_000068.7 | 2 | 60914664 | + | Rbms1 | NNNGCCACCTCACAAAGAAA | NGT | 2 | 0.0108 | Tier II |
16 | NC_000067.6 | 1 | 185465291 | - | Slc30a10 | NNNGCCACCTCACAAAGCAA | NGC | 2 | 0.0095 | Tier II |
17 | NC_000082.6 | 16 | 26267255 | - | Gm41436 | NNNGCCATCTCACAAAGTAG | NGG | 2 | 0.6691 | Tier III |
18 | NC_000078.6 | 12 | 25189051 | + | Gm36287 | NNNGCCAGCTGACAAAGTAA | NGG | 2 | 0.1607 | Tier III |
19 | NC_000078.6 | 12 | 25189051 | + | Gm36496 | NNNGCCAGCTGACAAAGTAA | NGG | 2 | 0.1607 | Tier III |