Construct: sgRNA BRDN0001148610

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACGGCCACCTCACAAAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K4 (9448)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76263

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 101869683 + MAP4K4 NNNGCCACCTCACAAAGTAA NGG 0 1.0 Tier I
2 NC_000003.12 3 133628444 - TOPBP1 NNNGCCACTTCACAAAGTAG NGG 2 0.6691 Tier I
3 NC_000008.11 8 112849510 + CSMD3 NNNGCAACCTAACAAAGTAA NGG 2 0.6964 Tier II
4 NC_000016.10 16 88299935 - ZNF469 NNNGCCACCACACAAAGAAA NGG 2 0.5714 Tier II
5 NC_000015.10 15 55278390 + RAB27A NNNGCCACCTCACAAAGAAG NGG 2 0.5098 Tier II
6 NC_000012.12 12 118037229 + RFC5 NNNGCCACCCCACAAAGTAG NGG 2 0.4078 Tier II
7 NC_000012.12 12 118037229 + WSB2 NNNGCCACCCCACAAAGTAG NGG 2 0.4078 Tier II
8 NC_000002.12 2 216687407 + IGFBP5 NNNGACACCTCACAGAGTAA NGG 2 0.3714 Tier II
9 NC_000008.11 8 56960830 - BPNT2 NNNGACTCCTCACAAAGTAA NGG 2 0.25 Tier II
10 NC_000008.11 8 10344765 - MSRA NNNACCACCTCACACAGTAA NGG 2 0.2455 Tier II
11 NC_000003.12 3 54971914 + CACNA2D3 NNNTCCACCTCACAGAGTAA NGG 2 0.2364 Tier II
12 NC_000013.11 13 92778602 + GPC5 NNNGCCACCTCACTAAGGAA NGG 2 0.1778 Tier II
13 NC_000016.10 16 4430322 - DNAJA3 NNNCCCACCTCCCAAAGTAA NGG 2 0.1393 Tier II
14 NC_000012.12 12 112307173 - HECTD4 NNNGCCCCCTCCCAAAGTAA NGG 2 0.1151 Tier II
15 NC_000022.11 22 43057672 + TTLL1 NNNGCCACCTCAAAAAGTAA NAG 2 0.0997 Tier II
16 NC_000009.12 9 14499392 - NFIB NNNGCCACCTCACACAGGAA NGG 2 0.0909 Tier II
17 NC_000015.10 15 29287466 - ENTREP2 NNNGCCACCTCAAAAGGTAA NGG 2 0.074 Tier II
18 NC_000005.10 5 95108408 + MCTP1 NNNGCAACCTCACAAAGTAA NGA 2 0.0645 Tier II
19 NC_000006.12 6 77472027 + LOC105377864 NNNGCAACCTCACAAAGTAA NGA 2 0.0645 Tier II
20 NC_000018.10 18 55476301 + TCF4 NNNGCCACCTCACAAAGTGA NGA 2 0.026 Tier II
21 NC_000021.9 21 37238189 - VPS26C NNNGCCACCTCACATTGTAA NGG 2 0.0 Tier II
22 NC_000006.12 6 81764378 + LOC105377873 NNNGCCACATCACAAAGAAA NGG 2 0.5714 Tier III
23 NC_000002.12 2 216687407 + LOC124900515 NNNGACACCTCACAGAGTAA NGG 2 0.3714 Tier III
24 NC_000016.10 16 53370118 + MPHOSPH10P1 NNNTCCAGCTCACAAAGTAA NGG 2 0.2338 Tier III
25 NC_000011.10 11 115937614 + LINC02703 NNNGCCAACTCACACAGTAA NGG 2 0.1773 Tier III
26 NC_000007.14 7 72866971 - SPDYE7P NNNTCCACCTCTCAAAGTAA NGG 2 0.1212 Tier III
27 NC_000023.11 X 20674761 + LOC124905257 NNNGCCACCTCAGAAAGTTA NGG 2 0.0734 Tier III
28 NC_000018.10 18 55476301 + TCF4-AS1 NNNGCCACCTCACAAAGTGA NGA 2 0.026 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 40009749 + Map4k4 NNNGCCACCTCACAAAGTAA NAG 1 0.2593 Tier I
2 NC_000082.6 16 75876587 + Samsn1 NNNTCCACCTGACAAAGTAA NGG 2 0.0909 Tier I
3 NC_000076.6 10 69731103 + Ank3 NNNGCCACCTCACAAAGTAG NGG 1 0.7647 Tier II
4 NC_000070.6 4 97623859 - Nfia NNNGCCACCTCACAAAGTTG NGG 2 0.4118 Tier II
5 NC_000084.6 18 21027311 + Rnf138 NNNGCCAAGTCACAAAGTAA NGG 2 0.4024 Tier II
6 NC_000081.6 15 38049956 + Ubr5 NNNGACAGCTCACAAAGTAA NGG 2 0.3673 Tier II
7 NC_000071.6 5 32065372 - Babam2 NNNGCAACCTCACAAAGTGA NGG 2 0.3482 Tier II
8 NC_000073.6 7 143209563 + Kcnq1 NNNGCCAGCTCACTAAGTAA NGG 2 0.3429 Tier II
9 NC_000075.6 9 41129797 + Ubash3b NNNGCCATCTCACAAAGTGA NGG 2 0.3281 Tier II
10 NC_000068.7 2 109230325 - Mettl15 NNNGCTACCTCACAAATTAA NGG 2 0.2321 Tier II
11 NC_000067.6 1 11302676 + Prex2 NNNGCCACATCACAAAGTAA NAG 2 0.2222 Tier II
12 NC_000077.6 11 4946939 - Nefh NNNGCCACCTGACAAAGTAG NGG 2 0.1912 Tier II
13 NC_000079.6 13 93703172 + Dmgdh NNNGGCACCTCACAAACTAA NGG 2 0.1412 Tier II
14 NC_000072.6 6 106669843 - Cntn4 NNNGCCACCTCCCAAATTAA NGG 2 0.0658 Tier II
15 NC_000068.7 2 60914664 + Rbms1 NNNGCCACCTCACAAAGAAA NGT 2 0.0108 Tier II
16 NC_000067.6 1 185465291 - Slc30a10 NNNGCCACCTCACAAAGCAA NGC 2 0.0095 Tier II
17 NC_000082.6 16 26267255 - Gm41436 NNNGCCATCTCACAAAGTAG NGG 2 0.6691 Tier III
18 NC_000078.6 12 25189051 + Gm36287 NNNGCCAGCTGACAAAGTAA NGG 2 0.1607 Tier III
19 NC_000078.6 12 25189051 + Gm36496 NNNGCCAGCTGACAAAGTAA NGG 2 0.1607 Tier III
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Other clones with same target sequence:

(none)