Construct: sgRNA BRDN0001148626

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGGTTGAGCACAGCATATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIP5K1 (9677)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76308

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 43582601 + PPIP5K1 NNNTTGAGCACAGCATATCG NGG 0 1.0 Tier I
2 NC_000006.12 6 126072348 + TRMT11 NNNTTGAGCACAGCATATTG NGG 1 0.4615 Tier II
3 NC_000003.12 3 169280677 - MECOM NNNTTGAGCACAGCATATTA NGG 2 0.4327 Tier II
4 NC_000008.11 8 88253463 - MMP16 NNNTTCATCACAGCATATCG NGG 2 0.4261 Tier II
5 NC_000002.12 2 137348417 - THSD7B NNNTTGAACACTGCATATCG NGG 2 0.3333 Tier II
6 NC_000012.12 12 95889999 - CCDC38 NNNTTGAGCCCAGCAAATCG NGG 2 0.303 Tier II
7 NC_000002.12 2 61932082 - COMMD1 NNNTTGAGGACAGCAGATCG NGG 2 0.2143 Tier II
8 NC_000011.10 11 77670057 + RSF1 NNNTTGAGCACAGCAGATTG NGG 2 0.1598 Tier II
9 NC_000007.14 7 69666833 + AUTS2 NNNTTGAGCCCAGCATATTG NGG 2 0.1538 Tier II
10 NC_000012.12 12 27634257 - PPFIBP1 NNNTTGAGCACAGCATTTCA NGG 2 0.125 Tier II
11 NC_000012.12 12 94986172 + NDUFA12 NNNTTGAGCACAGCATATTG NAG 2 0.1197 Tier II
12 NC_000005.10 5 13904842 + DNAH5 NNNTTGAGCCCAGCAGATCG NGG 2 0.1154 Tier II
13 NC_000021.9 21 6446516 + LOC102724560 NNNTTGAGCACAGCAGGTCG NGG 2 0.0611 Tier II
14 NC_000021.9 21 43054838 + CBS NNNTTGAGCACAGCAGGTCG NGG 2 0.0611 Tier II
15 NC_000003.12 3 138827610 + PIK3CB NNNTTGAGCACAGCAGTTCG NGG 2 0.0462 Tier II
16 NC_000010.11 10 73391543 + ANXA7 NNNTTGAGCCCAGCATTTCG NGG 2 0.0444 Tier II
17 NC_000023.11 X 6963737 - PUDP NNNTTGAGCACAGCATTTGG NGG 2 0.0167 Tier II
18 NC_000002.12 2 151258632 + RBM43 NNNTTGAGCACAGGAGATCG NGG 2 0.0 Tier II
19 NC_000015.10 15 78574904 + CHRNA5 NNNTTGAGCACAGGAGATCG NGG 2 0.0 Tier II
20 NC_000019.10 19 5023851 - KDM4B NNNTTGAGCACAGGAGATCG NGG 2 0.0 Tier II
21 NC_000017.11 17 17926102 + TOM1L2 NNNTTGAGCACAGGATGTCG NGG 2 0.0 Tier II
22 NC_000003.12 3 146198488 - PLSCR4 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
23 NC_000005.10 5 150360824 - TCOF1 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
24 NC_000006.12 6 25293473 - CARMIL1 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
25 NC_000006.12 6 97261899 - MMS22L NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
26 NC_000013.11 13 48050315 + NUDT15 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
27 NC_000017.11 17 19576438 - SLC47A1 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
28 NC_000018.10 18 24126118 + TTC39C NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier II
29 NC_000015.10 15 43682436 + PPIP5K1P1 NNNTTGAGCACAGCATATCG NGG 0 1.0 Tier III
30 NC_000015.10 15 43682436 + PPIP5K1P1-CATSPER2 NNNTTGAGCACAGCATATCG NGG 0 1.0 Tier III
31 NC_000003.12 3 169280677 - LOC105374206 NNNTTGAGCACAGCATATTA NGG 2 0.4327 Tier III
32 NC_000002.12 2 199625227 + LINC01877 NNNTTGAGCACAGCATAGCA NGG 2 0.3125 Tier III
33 NC_000002.12 2 58987732 + LINC01122 NNNTTGAGCATAGCATATAG NGG 2 0.1319 Tier III
34 NC_000012.12 12 27122531 - LOC124902904 NNNTTGAGCACAGCATTTCA NGG 2 0.125 Tier III
35 NC_000008.11 8 80221670 + LOC105375920 NNNTTGAGCCCAGCAGATCG NGG 2 0.1154 Tier III
36 NC_000021.9 21 6446516 + LOC102724701 NNNTTGAGCACAGCAGGTCG NGG 2 0.0611 Tier III
37 NC_000008.11 8 101056905 - SLEAR NNNTTGAGCACAGGAAATCG NGG 2 0.0 Tier III
38 NC_000003.12 3 159964928 - IL12A-AS1 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier III
39 NC_000013.11 13 104855311 - LOC105370342 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier III
40 NC_000013.11 13 104855311 - LOC107984608 NNNTTGAGCCCAGGATATCG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 120066048 - Sptbn5 NNNTCGAGCTCAGCATATCG NGG 2 0.8824 Tier I
2 NC_000068.7 2 121349211 + Ppip5k1 NNNTTGAGGACAGCATATCT NGG 2 0.4333 Tier I
3 NC_000069.6 3 158274803 - Lrrc7 NNNATGAGCACAGCATATCA NGG 2 0.4464 Tier II
4 NC_000079.6 13 91040354 + Atg10 NNNTAGATCACAGCATATCG NGG 2 0.3125 Tier II
5 NC_000073.6 7 121014698 - Mfsd13b NNNTTGAGCACAGCATAACC NGG 2 0.2857 Tier II
6 NC_000079.6 13 24515308 - Ripor2 NNNTTGAGCACAGCAGATAG NGG 2 0.1484 Tier II
7 NC_000077.6 11 29548035 - Clhc1 NNNTTGAGCACATCATTTCG NGG 2 0.04 Tier II
8 NC_000077.6 11 29548035 - Rps27a NNNTTGAGCACATCATTTCG NGG 2 0.04 Tier II
9 NC_000068.7 2 120066048 - Gm52559 NNNTCGAGCTCAGCATATCG NGG 2 0.8824 Tier III
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Other clones with same target sequence:

(none)