Construct: sgRNA BRDN0001148639
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATAATCTGAACCCCACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K4 (4216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76510
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
161070632 |
- |
MAP3K4 |
NNNTAATCTGAACCCCACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
38727105 |
+ |
SCN10A |
NNNTAAGCTGAGCCCCACAT |
NGG |
2 |
0.4248 |
Tier II |
| 3 |
NC_000009.12 |
9 |
36381123 |
- |
RNF38 |
NNNAAATCTGAACCCCAAAT |
NGG |
2 |
0.2564 |
Tier II |
| 4 |
NC_000022.11 |
22 |
42925762 |
+ |
PACSIN2 |
NNNTAATCTAAACCACACAT |
NGG |
2 |
0.2074 |
Tier II |
| 5 |
NC_000001.11 |
1 |
38872054 |
+ |
MYCBP |
NNNTTATCTGAACCCCAAAT |
NGG |
2 |
0.1958 |
Tier II |
| 6 |
NC_000023.11 |
X |
65681893 |
+ |
MSN |
NNNTTCTCTGAACCCCACAT |
NGG |
2 |
0.1653 |
Tier II |
| 7 |
NC_000014.9 |
14 |
74886129 |
- |
DLST |
NNNTAATCTGAAACACACAT |
NGG |
2 |
0.0855 |
Tier II |
| 8 |
NC_000002.12 |
2 |
85419880 |
- |
SH2D6 |
NNNTAATATGAACCCCACAT |
NTG |
2 |
0.0253 |
Tier II |
| 9 |
NC_000023.11 |
X |
132118910 |
- |
FRMD7 |
NNNTAATCTGAACCCCAGAT |
NGC |
2 |
0.003 |
Tier II |
| 10 |
NC_000006.12 |
6 |
151630231 |
+ |
LOC124901435 |
NNNTAATCTGAACCCCACTT |
NGG |
1 |
0.5385 |
Tier III |
| 11 |
NC_000005.10 |
5 |
176182001 |
- |
CEP192P1 |
NNNTAATGTGAAACCCACAT |
NGG |
2 |
0.2473 |
Tier III |
| 12 |
NC_000001.11 |
1 |
38872054 |
+ |
GJA9-MYCBP |
NNNTTATCTGAACCCCAAAT |
NGG |
2 |
0.1958 |
Tier III |
| 13 |
NC_000001.11 |
1 |
38872054 |
+ |
RRAGC-DT |
NNNTTATCTGAACCCCAAAT |
NGG |
2 |
0.1958 |
Tier III |
| 14 |
NC_000004.12 |
4 |
176438083 |
- |
LOC124900817 |
NNNTAATCTGGACCACACAT |
NGG |
2 |
0.1444 |
Tier III |
| 15 |
NC_000023.11 |
X |
113234986 |
- |
LOC101928437 |
NNNTAATCTGAACCACAAAT |
NGG |
2 |
0.1197 |
Tier III |
| 16 |
NC_000008.11 |
8 |
48653533 |
- |
LOC101929268 |
NNNTAATCTGAAACCCACAG |
NGG |
2 |
0.0679 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
12264125 |
+ |
Map3k4 |
NNNTAATCCGAGCCCCACAT |
NGG |
2 |
0.6667 |
Tier I |
| 2 |
NC_000082.6 |
16 |
78563888 |
+ |
D16Ertd472e |
NNNTGAACTGAACCCCACAT |
NGG |
2 |
0.63 |
Tier II |
| 3 |
NC_000067.6 |
1 |
192738063 |
+ |
Hhat |
NNNTAAACTGAATCCCACAT |
NGG |
2 |
0.6125 |
Tier II |
| 4 |
NC_000073.6 |
7 |
84296124 |
- |
Arnt2 |
NNNTAAGCTGAACCCCACTT |
NGG |
2 |
0.3167 |
Tier II |
| 5 |
NC_000078.6 |
12 |
111993124 |
+ |
Tdrd9 |
NNNTATTCTGAAACCCACAT |
NGG |
2 |
0.2747 |
Tier II |
| 6 |
NC_000071.6 |
5 |
35996464 |
+ |
Afap1 |
NNNTAATCTGTACCCCAAAT |
NGG |
2 |
0.1657 |
Tier II |
| 7 |
NC_000079.6 |
13 |
59506587 |
+ |
Agtpbp1 |
NNNTAAACTGAACCCCACAG |
NGG |
2 |
0.1544 |
Tier II |
| 8 |
NC_000082.6 |
16 |
78563888 |
+ |
4930478L05Rik |
NNNTGAACTGAACCCCACAT |
NGG |
2 |
0.63 |
Tier III |
| 9 |
NC_000082.6 |
16 |
78563888 |
+ |
LOC115488614 |
NNNTGAACTGAACCCCACAT |
NGG |
2 |
0.63 |
Tier III |
| 10 |
NC_000072.6 |
6 |
140750924 |
+ |
Gm30215 |
NNNTAATCTGAACCCCACTA |
NGG |
2 |
0.3029 |
Tier III |
| 11 |
NC_000067.6 |
1 |
57212568 |
+ |
BC055402 |
NNNTAATCTGAACATCACAT |
NGG |
2 |
0.0233 |
Tier III |
Other clones with same target sequence:
(none)
