Construct: sgRNA BRDN0001148653
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAGACGATGTCATCCAGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKM (1158)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78030
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
45319541 |
- |
CKM |
NNNGACGATGTCATCCAGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
103522321 |
- |
CKB |
NNNGACGACGTCATCCAGAC |
NGG |
1 |
0.9231 |
Tier I |
| 3 |
NC_000023.11 |
X |
49237741 |
- |
CCDC22 |
NNNGACCATGTCATCCAGAC |
NGG |
1 |
0.6875 |
Tier II |
| 4 |
NC_000011.10 |
11 |
58588443 |
- |
ZFP91 |
NNNGAGGATGTCATCCAGAC |
NGG |
1 |
0.5 |
Tier II |
| 5 |
NC_000005.10 |
5 |
138760987 |
+ |
CTNNA1 |
NNNGATGATGTCATCCAGAA |
NGG |
2 |
0.4643 |
Tier II |
| 6 |
NC_000016.10 |
16 |
70946603 |
+ |
HYDIN |
NNNGACCATGGCATCCAGAC |
NGG |
2 |
0.275 |
Tier II |
| 7 |
NC_000012.12 |
12 |
31490585 |
- |
DENND5B |
NNNGACTCTGTCATCCAGAC |
NGG |
2 |
0.2449 |
Tier II |
| 8 |
NC_000010.11 |
10 |
119320029 |
+ |
GRK5 |
NNNGAGGATGTGATCCAGAC |
NGG |
2 |
0.2222 |
Tier II |
| 9 |
NC_000002.12 |
2 |
137643407 |
- |
THSD7B |
NNNGACAATGTCATCCTGAC |
NGG |
2 |
0.1333 |
Tier II |
| 10 |
NC_000005.10 |
5 |
53109086 |
- |
MOCS2 |
NNNGACGATGTTATACAGAC |
NGG |
2 |
0.1197 |
Tier II |
| 11 |
NC_000022.11 |
22 |
19087588 |
+ |
DGCR2 |
NNNCACGATGTCAGCCAGAC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000011.10 |
11 |
58588443 |
- |
ZFP91-CNTF |
NNNGAGGATGTCATCCAGAC |
NGG |
1 |
0.5 |
Tier III |
| 13 |
NC_000001.11 |
1 |
146770840 |
- |
HYDIN2 |
NNNGACCATGGCATCCAGAC |
NGG |
2 |
0.275 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
19414311 |
+ |
Ckm |
NNNGACGATGTCATCCAGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
80026874 |
+ |
Arhgap45 |
NNNGACGATGTCATCCAGTG |
NGG |
2 |
0.0317 |
Tier I |
| 3 |
NC_000079.6 |
13 |
108088745 |
- |
Ndufaf2 |
NNNGACAATGTCATCAAGAC |
NGG |
2 |
1.0 |
Tier II |
| 4 |
NC_000072.6 |
6 |
137079568 |
+ |
Rerg |
NNNGACAGTGTCATCCAGAC |
NGG |
2 |
0.7333 |
Tier II |
| 5 |
NC_000083.6 |
17 |
44510070 |
+ |
Runx2 |
NNNGAAGATGTCATCCAGAC |
NTG |
2 |
0.0362 |
Tier II |
| 6 |
NC_000076.6 |
10 |
67702465 |
+ |
Gm32438 |
NNNCACGAAGTCATCCAGAC |
NGG |
2 |
0.4916 |
Tier III |
| 7 |
NC_000083.6 |
17 |
53746312 |
+ |
Ckb-ps2 |
NNNGACGATGCCATTCAGAC |
NGG |
2 |
0.0444 |
Tier III |
Other clones with same target sequence:
(none)
