Construct: sgRNA BRDN0001148657
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACATAGGCACAATGTGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIOK2 (55781)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76959
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
97177170 |
- |
RIOK2 |
NNNATAGGCACAATGTGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
17078149 |
+ |
CUBN |
NNNACAGGCACAATGTGACA |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000010.11 |
10 |
95433806 |
+ |
SORBS1 |
NNNAAACGCACAATGTGTCA |
NGG |
2 |
0.3438 |
Tier II |
| 4 |
NC_000002.12 |
2 |
79789323 |
+ |
CTNNA2 |
NNNGAAGGCACAATGTGTCA |
NGG |
2 |
0.3125 |
Tier II |
| 5 |
NC_000022.11 |
22 |
35970218 |
- |
RBFOX2 |
NNNATAGGAATAATGTGTCA |
NGG |
2 |
0.2637 |
Tier II |
| 6 |
NC_000006.12 |
6 |
151524774 |
- |
CCDC170 |
NNNATAGGCACAATGTGTAT |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000005.10 |
5 |
79245602 |
+ |
JMY |
NNNATAGGCACAGTGTGGCA |
NGG |
2 |
0.2174 |
Tier II |
| 8 |
NC_000003.12 |
3 |
183678584 |
+ |
KLHL24 |
NNNATAGGTACACTGTGTCA |
NGG |
2 |
0.1842 |
Tier II |
| 9 |
NC_000015.10 |
15 |
20845539 |
- |
POTEB2 |
NNNATAGGTACACTGTGTCA |
NGG |
2 |
0.1842 |
Tier II |
| 10 |
NC_000005.10 |
5 |
20271086 |
- |
CDH18 |
NNNATAGGCAAACTGTGTCA |
NGG |
2 |
0.1579 |
Tier II |
| 11 |
NC_000003.12 |
3 |
44359871 |
- |
TCAIM |
NNNCTAGGCACATTGTGTCA |
NGG |
2 |
0.1059 |
Tier II |
| 12 |
NC_000006.12 |
6 |
87184207 |
+ |
ZNF292 |
NNNATTGGCACAATGTGTGA |
NGG |
2 |
0.0893 |
Tier II |
| 13 |
NC_000009.12 |
9 |
70279904 |
+ |
SMC5 |
NNNATAGGCACATTGTGTCA |
NAG |
2 |
0.0778 |
Tier II |
| 14 |
NC_000008.11 |
8 |
55340309 |
- |
XKR4 |
NNNATATGCACAATGTGTCA |
NGA |
2 |
0.0397 |
Tier II |
| 15 |
NC_000008.11 |
8 |
40890802 |
- |
ZMAT4 |
NNNATTGGCACAATGTGTCA |
NTG |
2 |
0.0278 |
Tier II |
| 16 |
NC_000008.11 |
8 |
94864152 |
+ |
INTS8 |
NNNATAGTCACAATGTGTCA |
NGT |
2 |
0.0101 |
Tier II |
| 17 |
NC_000003.12 |
3 |
101996296 |
+ |
RDUR |
NNNATAAGAACAATGTGTCA |
NGG |
2 |
0.8571 |
Tier III |
| 18 |
NC_000008.11 |
8 |
144502680 |
+ |
LOC101928953 |
NNNCTTGGCACAATGTGTCA |
NGG |
2 |
0.2521 |
Tier III |
| 19 |
NC_000011.10 |
11 |
39706708 |
- |
LOC105376637 |
NNNATAGGCAACATGTGTCA |
NGG |
2 |
0.1974 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
124594866 |
- |
Fgf14 |
NNNATGGGCACAATGTGTCT |
NGG |
2 |
0.4286 |
Tier II |
| 2 |
NC_000078.6 |
12 |
89539915 |
- |
Nrxn3 |
NNNATAGGCACAGAGTGTCA |
NGG |
2 |
0.4037 |
Tier II |
| 3 |
NC_000069.6 |
3 |
55899450 |
+ |
Nbea |
NNNATAGTCACAATGTGCCA |
NGG |
2 |
0.2679 |
Tier II |
| 4 |
NC_000079.6 |
13 |
54959196 |
+ |
Unc5a |
NNNACAGGCAGAATGTGTCA |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000078.6 |
12 |
53824780 |
- |
Npas3 |
NNNATAGCCAGAATGTGTCA |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000086.7 |
X |
161909656 |
- |
Nhs |
NNNATAGGCACACAGTGTCA |
NGG |
2 |
0.1303 |
Tier II |
| 7 |
NC_000072.6 |
6 |
64386074 |
- |
Grid2 |
NNNATGGGCACAATGTGTGA |
NGG |
2 |
0.0893 |
Tier II |
| 8 |
NC_000084.6 |
18 |
22771462 |
- |
Nol4 |
NNNATAGACACAATGTGTCA |
NGA |
2 |
0.0694 |
Tier II |
| 9 |
NC_000084.6 |
18 |
79041778 |
+ |
Setbp1 |
NNNATATGCACAATGTGTCA |
NGC |
2 |
0.0127 |
Tier II |
| 10 |
NC_000076.6 |
10 |
41317412 |
+ |
Ak9 |
NNNATAGGCACAATGTGTCA |
NAC |
2 |
0.0 |
Tier II |
| 11 |
NC_000086.7 |
X |
139806995 |
+ |
Cldn2 |
NNNATAGGCACAGGGTGTCA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000069.6 |
3 |
55899450 |
+ |
Gm31914 |
NNNATAGTCACAATGTGCCA |
NGG |
2 |
0.2679 |
Tier III |
| 13 |
NC_000083.6 |
17 |
87277472 |
+ |
4833418N02Rik |
NNNATAGACACCATGTGTCA |
NGG |
2 |
0.2632 |
Tier III |
Other clones with same target sequence:
(none)
