Construct: sgRNA BRDN0001148660
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGGGAAATCCGAATGCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL1 (8814)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76267
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50395706 |
- |
CDKL1 |
NNNGGAAATCCGAATGCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
89400247 |
- |
NOX4 |
NNNGGAAATCCGAATGGTCA |
NAG |
2 |
0.0153 |
Tier I |
| 3 |
NC_000003.12 |
3 |
124234185 |
- |
KALRN |
NNNAGAAATCAGAATGCTCA |
NGG |
2 |
0.675 |
Tier II |
| 4 |
NC_000008.11 |
8 |
40572719 |
- |
ZMAT4 |
NNNGGAAATGCAAATGCTCA |
NGG |
2 |
0.363 |
Tier II |
| 5 |
NC_000003.12 |
3 |
38382544 |
- |
XYLB |
NNNGGCAATCAGAATGCTCA |
NGG |
2 |
0.3409 |
Tier II |
| 6 |
NC_000004.12 |
4 |
65439827 |
+ |
EPHA5 |
NNNGGAAAGCCGAATGCTTA |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000008.11 |
8 |
107365612 |
- |
ANGPT1 |
NNNCGAAATCCCAATGCTCA |
NGG |
2 |
0.2803 |
Tier II |
| 8 |
NC_000005.10 |
5 |
137695505 |
+ |
KLHL3 |
NNNGGAAATCAGAATGCTGA |
NGG |
2 |
0.0938 |
Tier II |
| 9 |
NC_000001.11 |
1 |
40688732 |
+ |
RIMS3 |
NNNGGAAATCCTAATGCTCA |
NTG |
2 |
0.015 |
Tier II |
| 10 |
NC_000005.10 |
5 |
149849898 |
- |
PPARGC1B |
NNNGGAAATCCGAATGATCA |
NGT |
2 |
0.0075 |
Tier II |
| 11 |
NC_000017.11 |
17 |
77834580 |
+ |
LOC105371908 |
NNNGGAAAGCCAAATGCTCA |
NGG |
2 |
0.5778 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
89165808 |
- |
Plxnb2 |
NNNGGAAGTCCGAGTGCTCA |
NGG |
2 |
0.3422 |
Tier I |
| 2 |
NC_000078.6 |
12 |
69789992 |
- |
Cdkl1 |
NNNAGAAATCCGCATGCTCA |
NGG |
2 |
0.1895 |
Tier I |
| 3 |
NC_000074.6 |
8 |
22162329 |
- |
Nek3 |
NNNGGAAATCAGACTGCTCA |
NGG |
2 |
0.1607 |
Tier I |
| 4 |
NC_000083.6 |
17 |
80560355 |
- |
Cdkl4 |
NNNGGAAATCCGTATGCTGA |
NGG |
2 |
0.0375 |
Tier I |
| 5 |
NC_000077.6 |
11 |
51345854 |
- |
Col23a1 |
NNNGGAAATCCAAATGCTCA |
NGG |
1 |
0.9333 |
Tier II |
Other clones with same target sequence:
(none)
