Construct: sgRNA BRDN0001148668
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGGCAATACGTACCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK2 (5610)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75639
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
37139633 |
+ |
EIF2AK2 |
NNNGGCAATACGTACCACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
24763025 |
- |
CLIC4 |
NNNAGCAATACATACCACTG |
NGG |
2 |
0.84 |
Tier II |
| 3 |
NC_000014.9 |
14 |
57729852 |
- |
SLC35F4 |
NNNGGCAAGACTTACCACTG |
NGG |
2 |
0.2381 |
Tier II |
| 4 |
NC_000003.12 |
3 |
60155898 |
- |
FHIT |
NNNGGCAATAAGTAACACTG |
NGG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000003.12 |
3 |
113339441 |
+ |
CFAP44 |
NNNGGCCATAGGTACCACTG |
NGG |
2 |
0.1094 |
Tier II |
| 6 |
NC_000003.12 |
3 |
113339441 |
+ |
LOC127898559 |
NNNGGCCATAGGTACCACTG |
NGG |
2 |
0.1094 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
117164422 |
- |
Ptprn2 |
NNNGGCAAAAGGTACCACTG |
NGG |
2 |
0.2321 |
Tier II |
| 2 |
NC_000079.6 |
13 |
59493073 |
+ |
Agtpbp1 |
NNNGGCCATACGTACCACTG |
NGA |
2 |
0.0304 |
Tier II |
| 3 |
NC_000067.6 |
1 |
90283899 |
+ |
2700003A03Rik |
NNNGGAAATAGGTACCACTG |
NGG |
2 |
0.2321 |
Tier III |
Other clones with same target sequence:
(none)
