Construct: sgRNA BRDN0001148688

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGAATCTGATGTATACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2G (818)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76237

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 73849328 + CAMK2G NNNGAATCTGATGTATACAG NGG 0 1.0 Tier I
2 NC_000002.12 2 72759138 - EXOC6B NNNGAAACTGAAGTATACAG NGG 2 0.7 Tier II
3 NC_000022.11 22 33742203 - LARGE1 NNNGAAACTGAAGTATACAG NGG 2 0.7 Tier II
4 NC_000017.11 17 37224606 - ACACA NNNGAATCAGATGTATACAT NGG 2 0.65 Tier II
5 NC_000018.10 18 10902522 - PIEZO2 NNNGAATCTGATGTAAACAT NGG 2 0.6364 Tier II
6 NC_000016.10 16 24732540 + TNRC6A NNNGAATTTGATGTATAAAG NGG 2 0.4712 Tier II
7 NC_000003.12 3 171173796 - TNIK NNNGAGTCTGGTGTATACAG NGG 2 0.4643 Tier II
8 NC_000016.10 16 27524502 + GTF3C1 NNNGAAACTGAGGTATACAG NGG 2 0.4375 Tier II
9 NC_000008.11 8 115456268 - TRPS1 NNNGAATAGGATGTATACAG NGG 2 0.4024 Tier II
10 NC_000017.11 17 42411866 - CAVIN1 NNNGAATGGGATGTATACAG NGG 2 0.398 Tier II
11 NC_000008.11 8 50001609 - SNTG1 NNNCAGTCTGATGTATACAG NGG 2 0.3782 Tier II
12 NC_000012.12 12 29746326 + TMTC1 NNNGAATATGATGTATAAAG NGG 2 0.35 Tier II
13 NC_000012.12 12 24999016 - IRAG2 NNNGCATATGATGTATACAG NGG 2 0.325 Tier II
14 NC_000011.10 11 85820663 + SYTL2 NNNGCATCTGAGGTATACAG NGG 2 0.25 Tier II
15 NC_000003.12 3 114735435 - ZBTB20 NNNGCATCTGCTGTATACAG NGG 2 0.2 Tier II
16 NC_000012.12 12 121427060 - KDM2B NNNGAATCTGATGTTTACAA NGG 2 0.1875 Tier II
17 NC_000014.9 14 99801309 - EML1 NNNGAATCTAATGTTTACAG NGG 2 0.1867 Tier II
18 NC_000020.11 20 34301494 - AHCY NNNGAAACTGATGTTTACAG NGG 2 0.175 Tier II
19 NC_000010.11 10 105147076 - SORCS3 NNNGCATCTGTTGTATACAG NGG 2 0.1538 Tier II
20 NC_000004.12 4 113677811 + CAMK2D NNNTAATCTCATGTATACAG NGG 2 0.1455 Tier II
21 NC_000006.12 6 157117076 + ARID1B NNNGAATCTGATGTTCACAG NGG 2 0.1333 Tier II
22 NC_000008.11 8 476006 + FBXO25 NNNGAATATGATGTTTACAG NGG 2 0.13 Tier II
23 NC_000001.11 1 232404911 + SIPA1L2 NNNGAATCTGAGGTATACAG NAG 2 0.1296 Tier II
24 NC_000006.12 6 79037839 - PHIP NNNGTATCTGATGTATACAG NAG 2 0.0943 Tier II
25 NC_000005.10 5 65388333 + ADAMTS6 NNNGCATCTGATGTATGCAG NGG 2 0.0882 Tier II
26 NC_000014.9 14 70646327 + TTC9 NNNGAATCTGATCTTTACAG NGG 2 0.0842 Tier II
27 NC_000004.12 4 26961551 + STIM2 NNNGAATCTGGTGTATACAG NGC 2 0.0144 Tier II
28 NC_000009.12 9 10589700 + PTPRD NNNGAAACTGATGGATACAG NGG 2 0.0 Tier II
29 NC_000022.11 22 33742203 - LARGE-AS1 NNNGAAACTGAAGTATACAG NGG 2 0.7 Tier III
30 NC_000007.14 7 25952984 - LOC105375199 NNNGAATCTGATGTACACAA NGG 2 0.625 Tier III
31 NC_000003.12 3 65182130 - LINC02040 NNNGAAGCTGATGTATACAG NGG 1 0.5882 Tier III
32 NC_000011.10 11 93956727 - LOC101060084 NNNGAAACTGAGGTATACAG NGG 2 0.4375 Tier III
33 NC_000014.9 14 97773791 - LOC105370651 NNNCAATGTGATGTATACAG NGG 2 0.3403 Tier III
34 NC_000007.14 7 13689213 - LOC107986770 NNNGAATATGATGTATACAG NAG 2 0.1685 Tier III
35 NC_000013.11 13 106337243 + LOC107984626 NNNGAAGCTGATGTATACAG NAG 2 0.1525 Tier III
36 NC_000006.12 6 79037839 - LOC124901346 NNNGTATCTGATGTATACAG NAG 2 0.0943 Tier III
37 NC_000010.11 10 7031170 + LOC105376387 NNNGAATCTGATGGATGCAG NGG 2 0.0 Tier III
38 NC_000012.12 12 93055889 + LOC643339 NNNGAATCTGATGTATACAG NAA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 20766220 + Camk2g NNNGGATCTGATGTATACAG NGG 1 0.72 Tier I
2 NC_000068.7 2 146502056 + Ralgapa2 NNNGAAACTGATGAATACAG NGG 2 0.5417 Tier II
3 NC_000071.6 5 136022772 + Dtx2 NNNGAATCTCATGTATACAG NGG 1 0.4 Tier II
4 NC_000070.6 4 140209438 + Igsf21 NNNGCATCTGCTGTATACAG NGG 2 0.2 Tier II
5 NC_000082.6 16 59455565 + Gabrr3 NNNGAATCTGATGTATACTG NAG 2 0.1396 Tier II
6 NC_000067.6 1 181802630 - Dnah14 NNNGAATGTGATGTTTACAG NGG 2 0.1286 Tier II
7 NC_000086.7 X 72599171 + Gabra3 NNNGAAACTGATGTATAGAG NGG 2 0.1167 Tier II
8 NC_000084.6 18 43326086 - Dpysl3 NNNGAATCTCATGTCTACAG NGG 2 0.1091 Tier II
9 NC_000076.6 10 117737586 - Slc35e3 NNNGAATCTGAGGTATAGAG NGG 2 0.0667 Tier II
10 NC_000072.6 6 53491390 - Creb5 NNNGAATCTGAAGTATACAG NGA 2 0.0556 Tier II
11 NC_000084.6 18 61566573 - Csnk1a1 NNNGAATCTGATGTTTACAG NAG 2 0.0519 Tier II
12 NC_000082.6 16 18452454 - Txnrd2 NNNGCATCTGATGTATACAG NGA 2 0.0347 Tier II
13 NC_000074.6 8 106626043 - Cdh1 NNNGAAGCTGATGGATACAG NGG 2 0.0 Tier II
14 NC_000081.6 15 24299064 - Gm36147 NNNGAACCTGATGTATACAG NGG 1 0.6875 Tier III
15 NC_000083.6 17 22792837 + Gm16386 NNNGTATCTGATGAATACAG NGG 2 0.2251 Tier III
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Other clones with same target sequence:

(none)