Construct: sgRNA BRDN0001148698
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTAAGGGCACGTGCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ARSG (22901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77642
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
68368592 |
- |
ARSG |
NNNTAAGGGCACGTGCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
169629640 |
+ |
MECOM |
NNNTAAGGGAACGTGCATGT |
NGG |
1 |
0.8667 |
Tier II |
| 3 |
NC_000004.12 |
4 |
48149140 |
- |
TEC |
NNNTAAAGACACGTGCATGT |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000003.12 |
3 |
79693022 |
+ |
ROBO1 |
NNNTAAGGTCAGGTGCATGT |
NGG |
2 |
0.237 |
Tier II |
| 5 |
NC_000009.12 |
9 |
125916170 |
- |
PBX3 |
NNNTAAGGGCACGTGAATGT |
NCG |
2 |
0.1071 |
Tier II |
| 6 |
NC_000008.11 |
8 |
22047664 |
+ |
FGF17 |
NNNTAAGGGCCCGTGCATGC |
NGG |
2 |
0.0364 |
Tier II |
| 7 |
NC_000023.11 |
X |
153690006 |
- |
SLC6A8 |
NNNTGAGGGCACGTGCATGT |
NTG |
2 |
0.0281 |
Tier II |
| 8 |
NC_000011.10 |
11 |
121102827 |
+ |
TECTA |
NNNTAAGGGCAGGTGCATGT |
NTG |
2 |
0.0173 |
Tier II |
| 9 |
NC_000011.10 |
11 |
121102827 |
+ |
TBCEL-TECTA |
NNNTAAGGGCAGGTGCATGT |
NTG |
2 |
0.0173 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
129241477 |
+ |
C77080 |
NNNTAATGGCACGTGCATGT |
NGG |
1 |
0.5714 |
Tier II |
| 2 |
NC_000068.7 |
2 |
5883436 |
- |
Sec61a2 |
NNNTAAGCGCACATGCATGT |
NGG |
2 |
0.568 |
Tier II |
| 3 |
NC_000079.6 |
13 |
12502116 |
- |
Edaradd |
NNNTGAGTGCACGTGCATGT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000081.6 |
15 |
10331050 |
- |
Prlr |
NNNTGAGGGCATGTGCATGT |
NGG |
2 |
0.3877 |
Tier II |
| 5 |
NC_000071.6 |
5 |
106626698 |
+ |
Zfp644 |
NNNTAAGGCCACGTGCATGT |
NTG |
2 |
0.021 |
Tier II |
| 6 |
NC_000071.6 |
5 |
142488084 |
+ |
Radil |
NNNTCAGGGCACGTGCATGT |
NTG |
2 |
0.0195 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48357928 |
+ |
Sorcs3 |
NNNTAAGGGCAGGTGCATGT |
NTG |
2 |
0.0173 |
Tier II |
| 8 |
NC_000079.6 |
13 |
11910072 |
+ |
Ryr2 |
NNNTAAGGGCACGTGCATGA |
NGC |
2 |
0.0125 |
Tier II |
| 9 |
NC_000070.6 |
4 |
129241477 |
+ |
Gm12976 |
NNNTAATGGCACGTGCATGT |
NGG |
1 |
0.5714 |
Tier III |
Other clones with same target sequence:
(none)
