Construct: sgRNA BRDN0001148838
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATGAGTATGACTTTGAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BTK (695)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77072
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
101375151 |
- |
BTK |
NNNGAGTATGACTTTGAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
84932811 |
+ |
NTRK2 |
NNNGAGTAAGAATTTGAACG |
NGG |
2 |
0.6633 |
Tier II |
| 3 |
NC_000016.10 |
16 |
82794108 |
+ |
CDH13 |
NNNGAGGATGACTTTGAATG |
NGG |
2 |
0.2715 |
Tier II |
| 4 |
NC_000002.12 |
2 |
97837188 |
- |
TMEM131 |
NNNGAATATGACTTTGAACG |
NAG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000011.10 |
11 |
84893596 |
+ |
DLG2 |
NNNGAGTCTCACTTTGAACG |
NGG |
2 |
0.1714 |
Tier II |
| 6 |
NC_000005.10 |
5 |
67005776 |
+ |
MAST4 |
NNNGACTATGACTTTGAAGG |
NGG |
2 |
0.0852 |
Tier II |
| 7 |
NC_000020.11 |
20 |
7984293 |
+ |
TMX4 |
NNNGAGGATGACTTTGAAGG |
NGG |
2 |
0.0735 |
Tier II |
| 8 |
NC_000016.10 |
16 |
82794108 |
+ |
LOC101928446 |
NNNGAGGATGACTTTGAATG |
NGG |
2 |
0.2715 |
Tier III |
| 9 |
NC_000004.12 |
4 |
143854054 |
- |
LOC105377459 |
NNNCAGTATGACTTTGAATG |
NGG |
2 |
0.2443 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
134573924 |
- |
Btk |
NNNGAATATGACTTTGAACG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
5975966 |
+ |
Upf2 |
NNNGAGTATGACTTAGAAGG |
NGG |
2 |
0.0724 |
Tier I |
| 3 |
NC_000073.6 |
7 |
102287152 |
+ |
Stim1 |
NNNGAGGATGACTTTGAACT |
NGG |
2 |
0.4118 |
Tier II |
| 4 |
NC_000069.6 |
3 |
103770879 |
+ |
Hipk1 |
NNNCAGTATGACTTTGAACT |
NGG |
2 |
0.3706 |
Tier II |
| 5 |
NC_000076.6 |
10 |
40676054 |
+ |
Ddo |
NNNGAGTTTGACCTTGAACG |
NGG |
2 |
0.3383 |
Tier II |
| 6 |
NC_000071.6 |
5 |
6995314 |
- |
Zfp804b |
NNNGAGTGTGACTTTGAAAG |
NGG |
2 |
0.3143 |
Tier II |
| 7 |
NC_000073.6 |
7 |
107130335 |
+ |
Nlrp14 |
NNNGACTATGACTTTGCACG |
NGG |
2 |
0.1203 |
Tier II |
| 8 |
NC_000073.6 |
7 |
87836758 |
- |
Grm5 |
NNNGAGTATGACTTTGACAG |
NGG |
2 |
0.0816 |
Tier II |
| 9 |
NC_000078.6 |
12 |
79355088 |
+ |
Rad51b |
NNNGAGTTTGACTTTGGACG |
NGG |
2 |
0.0756 |
Tier II |
| 10 |
NC_000074.6 |
8 |
41308902 |
+ |
Pcm1 |
NNNGAGTATGACTTTGAAAG |
NGT |
2 |
0.0069 |
Tier II |
Other clones with same target sequence:
(none)
