Construct: sgRNA BRDN0001148840
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATGCGGCAGAGATCCTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK5 (2869)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76274
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
119436800 |
+ |
GRK5 |
NNNGCGGCAGAGATCCTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
205774454 |
- |
RAB29 |
NNNGCAGCTGAGATCCTCTG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000005.10 |
5 |
115262268 |
- |
PGGT1B |
NNNGCGGCAGAGAACCTCTA |
NGG |
2 |
0.5804 |
Tier II |
| 4 |
NC_000023.11 |
X |
21842843 |
- |
MBTPS2 |
NNNGCGACAGAGACCCTCTG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000018.10 |
18 |
628457 |
+ |
CLUL1 |
NNNGCGGCAGATATCTTCTG |
NGG |
2 |
0.1183 |
Tier II |
| 6 |
NC_000021.9 |
21 |
36852137 |
+ |
HLCS |
NNNGCTGCAGAGATGCTCTG |
NGG |
2 |
0.0333 |
Tier II |
| 7 |
NC_000013.11 |
13 |
40385210 |
- |
LINC00598 |
NNNGAGGCAGAGATCATCTG |
NGG |
2 |
0.5714 |
Tier III |
| 8 |
NC_000004.12 |
4 |
180459292 |
+ |
LOC105377567 |
NNNGCGGCAAACATCCTCTG |
NGG |
2 |
0.4941 |
Tier III |
| 9 |
NC_000018.10 |
18 |
628457 |
+ |
LOC105371952 |
NNNGCGGCAGATATCTTCTG |
NGG |
2 |
0.1183 |
Tier III |
| 10 |
NC_000009.12 |
9 |
134854046 |
- |
LOC101448202 |
NNNGAGGCAGAGATCCTCTG |
NGA |
2 |
0.0397 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
61076787 |
+ |
Grk5 |
NNNGCAGCTGAGATCCTCTG |
NGG |
2 |
0.6 |
Tier I |
| 2 |
NC_000085.6 |
19 |
61076787 |
+ |
Zfp950 |
NNNGCAGCTGAGATCCTCTG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000077.6 |
11 |
113483457 |
- |
Slc39a11 |
NNNGCATCAGAGATCCTCTG |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000075.6 |
9 |
45174656 |
+ |
Tmprss4 |
NNNGCTCCAGAGATCCTCTG |
NGG |
2 |
0.4583 |
Tier II |
| 5 |
NC_000081.6 |
15 |
64186215 |
- |
Asap1 |
NNNGGGGAAGAGATCCTCTG |
NGG |
2 |
0.39 |
Tier II |
| 6 |
NC_000069.6 |
3 |
154017937 |
- |
Slc44a5 |
NNNGCGGAAGAGATCCACTG |
NGG |
2 |
0.3467 |
Tier II |
| 7 |
NC_000072.6 |
6 |
87308834 |
- |
Antxr1 |
NNNGCAGCAGAGACCCTCTG |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000076.6 |
10 |
59051700 |
- |
Sh3rf3 |
NNNGCAGCAGAGACCCTCTG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000077.6 |
11 |
68893500 |
+ |
Rnf222 |
NNNGGGGCAGAGATACTCTG |
NGG |
2 |
0.1333 |
Tier II |
| 10 |
NC_000073.6 |
7 |
78461781 |
- |
Ntrk3 |
NNNGAGGCAGAGCTCCTCTG |
NGG |
2 |
0.1203 |
Tier II |
| 11 |
NC_000073.6 |
7 |
68048877 |
+ |
Igf1r |
NNNGCAGCAGAGATGCTCTG |
NGG |
2 |
0.05 |
Tier II |
| 12 |
NC_000083.6 |
17 |
34972040 |
+ |
Hspa1a |
NNNGCGGCAGGGATGCTCTG |
NGG |
2 |
0.0325 |
Tier II |
| 13 |
NC_000078.6 |
12 |
100147651 |
+ |
Nrde2 |
NNNGCCGCAGAGAGCCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000071.6 |
5 |
141485092 |
+ |
Sdk1 |
NNNGCGGCAGAGAGGCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000077.6 |
11 |
112891617 |
+ |
Gm46301 |
NNNGGGGCAGAGATCCTCTG |
NGG |
1 |
0.6 |
Tier III |
Other clones with same target sequence:
(none)
