Construct: sgRNA BRDN0001148876

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAAGAAGGGCTACAACCCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSSK4 (283629)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77044

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 24207243 - TSSK4 NNNGAAGGGCTACAACCCAC NGG 0 1.0 Tier I
2 NC_000001.11 1 119625920 - ZNF697 NNNGATGGGCTCCAACCCAC NGG 2 0.188 Tier I
3 NC_000016.10 16 14046382 - MRTFB NNNGATGGGCTACAACCCAC NGG 1 0.7143 Tier II
4 NC_000003.12 3 56796541 - ARHGEF3 NNNGAACGGCTGCAACCCAC NGG 2 0.4965 Tier II
5 NC_000005.10 5 153747779 - GRIA1 NNNGAAAGGCTACACCCCAC NGG 2 0.2727 Tier II
6 NC_000004.12 4 20734824 + KCNIP4 NNNTAAGGGCTACAACCCTC NGG 2 0.1958 Tier II
7 NC_000004.12 4 20734824 + PACRGL NNNTAAGGGCTACAACCCTC NGG 2 0.1958 Tier II
8 NC_000010.11 10 125994912 - FANK1 NNNGAAGGGCTTCGACCCAC NGG 2 0.1556 Tier II
9 NC_000022.11 22 19984149 - ARVCF NNNGAGGGGCTACATCCCAC NGG 2 0.1429 Tier II
10 NC_000011.10 11 204961 + BET1L NNNGAAGGGCTGCAAGCCAC NGG 2 0.1111 Tier II
11 NC_000017.11 17 72871902 - SLC39A11 NNNGAAGGGCTACATCCCAC NAG 2 0.0519 Tier II
12 NC_000002.12 2 117819244 - DDX18 NNNGAAGGGCAACAACCCAC NTG 2 0.0292 Tier II
13 NC_000001.11 1 160841096 + CD244 NNNGAAGGGCTACAAGGCAC NGG 2 0.009 Tier II
14 NC_000004.12 4 48256269 + TEC NNNGAAGGGCTACAATCCAC NGC 2 0.0068 Tier II
15 NC_000012.12 12 114736925 - TBX3-AS1 NNNGAAAGGCTCCAACCCAC NGG 2 0.2632 Tier III
16 NC_000016.10 16 65481860 + LINC00922 NNNGAAGGGGTACCACCCAC NGG 2 0.0833 Tier III
17 NC_000006.12 6 134705152 - LOC101928277 NNNGCAGGGCTAGAACCCAC NGG 2 0.0682 Tier III
18 NC_000014.9 14 47777269 - LINC00648 NNNGAAGGGCTGCAACCCAC NGA 2 0.0502 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 80722228 - Gria2 NNNGAAAGGTTACAACCCAC NGG 2 0.9412 Tier II
2 NC_000072.6 6 118745598 - Cacna1c NNNAAAGGGCTACAACCCAC NGG 1 0.9 Tier II
3 NC_000073.6 7 81439205 - Cpeb1 NNNAAAGGGTTACAACCCAC NGG 2 0.8471 Tier II
4 NC_000067.6 1 62627207 + Pard3b NNNGAAGGGTTGCAACCCAC NGG 2 0.6797 Tier II
5 NC_000071.6 5 35072785 - Dok7 NNNAAAGGGCTGCAACCCAC NGG 2 0.65 Tier II
6 NC_000071.6 5 92688197 + Shroom3 NNNAAGGGGCTACAACCCAC NGG 2 0.6429 Tier II
7 NC_000080.6 14 55501938 - Carmil3 NNNGAAGGGTCACAACCCAC NGG 2 0.6275 Tier II
8 NC_000083.6 17 78680494 + Strn NNNAAAGGGCCACAACCCAC NGG 2 0.6 Tier II
9 NC_000075.6 9 107155539 + Dock3 NNNGAAGGGCTACTACCCAC NGG 1 0.5333 Tier II
10 NC_000083.6 17 46642861 + Klc4 NNNCAAGGGTTACAACCCAC NGG 2 0.4983 Tier II
11 NC_000078.6 12 21334543 - Adam17 NNNGCAGGGTTACAACCCAC NGG 2 0.4706 Tier II
12 NC_000068.7 2 93982235 + Alkbh3 NNNGAAGGGTTACAACTCAC NGG 2 0.4392 Tier II
13 NC_000075.6 9 103498222 + Tmem108 NNNGAAGAGCTAAAACCCAC NGG 2 0.3846 Tier II
14 NC_000086.7 X 18432287 + Dipk2b NNNGAAGGGCAACAACACAC NGG 2 0.35 Tier II
15 NC_000071.6 5 128199042 - Tmem132d NNNGAAGGGATAAAACCCAC NGG 2 0.3333 Tier II
16 NC_000071.6 5 118082265 + Fbxw8 NNNGAAGTGGTACAACCCAC NGG 2 0.2431 Tier II
17 NC_000076.6 10 64294111 + Ctnna3 NNNGAAGGGCTTTAACCCAC NGG 2 0.2333 Tier II
18 NC_000076.6 10 94039271 - Fgd6 NNNGAAGGGTTACAACCGAC NGG 2 0.1255 Tier II
19 NC_000082.6 16 13404599 - Mrtfb NNNGAAGGGCTACATCCCTC NGG 2 0.1077 Tier II
20 NC_000067.6 1 36413782 - Fer1l5 NNNGTAGGGCTCCAACCCAC NGG 2 0.0957 Tier II
21 NC_000067.6 1 91053292 + Lrrfip1 NNNGAAGGGGTACAACCCCC NGG 2 0.0805 Tier II
22 NC_000076.6 10 74291368 + Pcdh15 NNNAAAGGGCTACAACCCAG NGG 2 0.0529 Tier II
23 NC_000085.6 19 23357669 + Mamdc2 NNNGAAGGGCTACAACCCAC NCT 2 0.0 Tier II
24 NC_000076.6 10 100136608 + Gm35533 NNNAAAGGGTTACAACCCAC NGG 2 0.8471 Tier III
25 NC_000076.6 10 56448634 - Gm36595 NNNAAGGGGCTACAACCCAC NGG 2 0.6429 Tier III
26 NC_000081.6 15 52448612 + Gm41322 NNNAAGGGGCTACAACCCAC NGG 2 0.6429 Tier III
27 NC_000068.7 2 93982235 + Alkbh3os1 NNNGAAGGGTTACAACTCAC NGG 2 0.4392 Tier III
28 NC_000075.6 9 103498222 + Gm16252 NNNGAAGAGCTAAAACCCAC NGG 2 0.3846 Tier III
29 NC_000072.6 6 92292794 + Gm30688 NNNGAAAGGCTTCAACCCAC NGG 2 0.3333 Tier III
30 NC_000070.6 4 39412178 - Gm17094 NNNGAAGTGCTACATCCCAC NGG 2 0.125 Tier III
31 NC_000082.6 16 13404599 - Gm15738 NNNGAAGGGCTACATCCCTC NGG 2 0.1077 Tier III
32 NC_000083.6 17 9456986 - Gm51424 NNNGAAGGGCTACAACCCAA NGT 2 0.0081 Tier III
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Other clones with same target sequence:

(none)