Construct: sgRNA BRDN0001148885
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGTATTCTGGTTATACGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R4 (30849)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75594
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
130733959 |
- |
PIK3R4 |
NNNTATTCTGGTTATACGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
101288203 |
- |
RNF149 |
NNNTATTCTGGTTATACTAA |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000002.12 |
2 |
167346853 |
+ |
B3GALT1 |
NNNCATTCTGGTTATATGGA |
NGG |
2 |
0.302 |
Tier II |
| 4 |
NC_000002.12 |
2 |
141633329 |
+ |
LRP1B |
NNNTATTCTGGTTATAAGGA |
NAG |
2 |
0.121 |
Tier II |
| 5 |
NC_000001.11 |
1 |
113877060 |
+ |
BCL2L15 |
NNNTATTCTGGTTATATGGA |
NAG |
2 |
0.121 |
Tier II |
| 6 |
NC_000003.12 |
3 |
25086182 |
+ |
RARB |
NNNTATTCTGGTTATAGGAA |
NGG |
2 |
0.042 |
Tier II |
| 7 |
NC_000001.11 |
1 |
1658431 |
- |
CDK11B |
NNNTATTCTGGTTGTACGGA |
NGA |
2 |
0.0324 |
Tier II |
| 8 |
NC_000001.11 |
1 |
1723775 |
- |
CDK11A |
NNNTATTCTGGTTGTACGGA |
NGA |
2 |
0.0324 |
Tier II |
| 9 |
NC_000008.11 |
8 |
103668419 |
- |
RIMS2 |
NNNTATTCTGGTTATACAGA |
NGC |
2 |
0.0154 |
Tier II |
| 10 |
NC_000002.12 |
2 |
141633329 |
+ |
LOC107985779 |
NNNTATTCTGGTTATAAGGA |
NAG |
2 |
0.121 |
Tier III |
| 11 |
NC_000001.11 |
1 |
113877060 |
+ |
AP4B1-AS1 |
NNNTATTCTGGTTATATGGA |
NAG |
2 |
0.121 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
79510762 |
+ |
Fnip2 |
NNNTATTCTAGTTATACAGA |
NGG |
2 |
0.6462 |
Tier II |
| 2 |
NC_000076.6 |
10 |
36591084 |
+ |
Hs3st5 |
NNNTATTCTGGGTATATGGA |
NGG |
2 |
0.2333 |
Tier II |
| 3 |
NC_000068.7 |
2 |
180708105 |
- |
Dido1 |
NNNTATTCTGGTTATATGGA |
NGT |
2 |
0.0075 |
Tier II |
| 4 |
NC_000069.6 |
3 |
79510762 |
+ |
Gm36442 |
NNNTATTCTAGTTATACAGA |
NGG |
2 |
0.6462 |
Tier III |
Other clones with same target sequence:
(none)
