Construct: sgRNA BRDN0001148908
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAATATTGATATCATTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R4 (30849)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75595
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
130733639 |
+ |
PIK3R4 |
NNNATATTGATATCATTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
51423614 |
- |
PRKG1 |
NNNATATTGAGATCATTACA |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000022.11 |
22 |
32907817 |
+ |
SYN3 |
NNNCTATTGATATCATTACA |
NGG |
2 |
0.3309 |
Tier II |
| 4 |
NC_000001.11 |
1 |
201093093 |
+ |
CACNA1S |
NNNCTATTGTTATCATTACG |
NGG |
2 |
0.3114 |
Tier II |
| 5 |
NC_000003.12 |
3 |
71400217 |
+ |
FOXP1 |
NNNTTATTGATATCATTACC |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000011.10 |
11 |
87077204 |
+ |
TMEM135 |
NNNATATTTATATCATTTCG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000014.9 |
14 |
74491983 |
- |
NPC2 |
NNNATAGTGATATCATTACC |
NGG |
2 |
0.2521 |
Tier II |
| 8 |
NC_000016.10 |
16 |
70775151 |
+ |
VAC14 |
NNNATATTGATATTATTCCG |
NGG |
2 |
0.1397 |
Tier II |
| 9 |
NC_000018.10 |
18 |
10702499 |
+ |
PIEZO2 |
NNNATATTGATATAATTACG |
NAG |
2 |
0.0907 |
Tier II |
| 10 |
NC_000015.10 |
15 |
101469962 |
- |
PCSK6 |
NNNATATTGATATCATCACG |
NCG |
2 |
0.0756 |
Tier II |
| 11 |
NC_000015.10 |
15 |
101469962 |
- |
LOC124903566 |
NNNATATTGATATCATCACG |
NCG |
2 |
0.0756 |
Tier II |
| 12 |
NC_000006.12 |
6 |
12879624 |
- |
PHACTR1 |
NNNCTATTGATATCATTACG |
NGT |
2 |
0.0057 |
Tier II |
| 13 |
NC_000012.12 |
12 |
40185038 |
+ |
LRRK2-DT |
NNNATATTGTTATCATTAAG |
NGG |
2 |
0.3782 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
21192939 |
- |
Cdh12 |
NNNATATTGATATTATTACA |
NGG |
2 |
0.6875 |
Tier II |
| 2 |
NC_000073.6 |
7 |
66167116 |
+ |
Chsy1 |
NNNATATTGCTATCATTACT |
NGG |
2 |
0.2333 |
Tier II |
| 3 |
NC_000077.6 |
11 |
45908505 |
- |
Clint1 |
NNNATATTTATATCATTAAG |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000074.6 |
8 |
123649361 |
- |
Gm35930 |
NNNATATTTATTTCATTACG |
NGG |
2 |
0.1778 |
Tier III |
Other clones with same target sequence:
(none)
