Construct: sgRNA BRDN0001148924

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGCTGCAATACCTACACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD2 (6046)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77984

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 32974680 + BRD2 NNNGCTGCAATACCTACACA NGG 0 1.0 Tier I
2 NC_000003.12 3 32424222 + CMTM7 NNNGCTGTAACACCTACACA NGG 2 0.5833 Tier II
3 NC_000012.12 12 18425789 + PIK3C2G NNNGCAGAAATACCTACACA NGG 2 0.5633 Tier II
4 NC_000005.10 5 153564609 + GRIA1 NNNGCTGCAACACTTACACA NGG 2 0.4889 Tier II
5 NC_000019.10 19 48124429 + LIG1 NNNGCAGCAATACCTACTCA NGG 2 0.4333 Tier II
6 NC_000010.11 10 48833377 + WDFY4 NNNGCTGGAACACCTACACA NGG 2 0.4286 Tier II
7 NC_000018.10 18 33097275 - CCDC178 NNNGCTGGAACACCTACACA NGG 2 0.4286 Tier II
8 NC_000011.10 11 73336894 + ARHGEF17 NNNGCTGGCATACCTACACA NGG 2 0.3673 Tier II
9 NC_000006.12 6 97011837 + KLHL32 NNNGCTGCAAAACCTAAACA NGG 2 0.35 Tier II
10 NC_000013.11 13 112511932 + TUBGCP3 NNNTCTCCAATACCTACACA NGG 2 0.25 Tier II
11 NC_000023.11 X 130066362 - ELF4 NNNGCTGCATTCCCTACACA NGG 2 0.2322 Tier II
12 NC_000017.11 17 14322623 - HS3ST3B1 NNNCCTGCAATACATACACA NGG 2 0.1853 Tier II
13 NC_000004.12 4 151690180 - GATB NNNGCTGCAATAGCTACACT NGG 2 0.0818 Tier II
14 NC_000001.11 1 222714182 + BROX NNNGCTGCACTACCTACCCA NGG 2 0.0635 Tier II
15 NC_000017.11 17 36188381 - LOC128966684 NNNGCTGCAATTCCTACAGA NGG 2 0.0417 Tier II
16 NC_000013.11 13 33447352 + STARD13 NNNGCTGCAACACCTAGACA NGG 2 0.0392 Tier II
17 NC_000020.11 20 1584578 + SIRPB1 NNNGCTGAAATACCTACACA NTG 2 0.0253 Tier II
18 NC_000006.12 6 15359181 - JARID2 NNNGCTGCTATACCTACACA NTG 2 0.0234 Tier II
19 NC_000017.11 17 45670469 - LINC02210-CRHR1 NNNGCTGTAATACCTACACA NGT 2 0.0141 Tier II
20 NC_000005.10 5 111700554 + STARD4-AS1 NNNACTGCAATGCCTACACA NGG 2 0.65 Tier III
21 NC_000012.12 12 18425789 + LOC124902891 NNNGCAGAAATACCTACACA NGG 2 0.5633 Tier III
22 NC_000010.11 10 48833377 + LOC105378299 NNNGCTGGAACACCTACACA NGG 2 0.4286 Tier III
23 NC_000011.10 11 73336894 + LOC124902710 NNNGCTGGCATACCTACACA NGG 2 0.3673 Tier III
24 NC_000017.11 17 45670469 - LOC105371802 NNNGCTGTAATACCTACACA NGT 2 0.0141 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 123450113 - Prss12 NNNGCTGCAATAACTACACA NGG 1 0.3846 Tier II
2 NC_000071.6 5 36949100 + Ppp2r2c NNNGCTGCTGTACCTACACA NGG 2 0.3333 Tier II
3 NC_000070.6 4 86908648 + Acer2 NNNGCTGTAATACATACACA NGG 2 0.3062 Tier II
4 NC_000069.6 3 155138683 + Lrriq3 NNNGCTGCACTACCAACACA NGG 2 0.193 Tier II
5 NC_000071.6 5 129729083 + Nipsnap2 NNNGCTGCAATACCTACTCC NGG 2 0.1136 Tier II
6 NC_000081.6 15 95802641 + Ano6 NNNGCTGCAATAGCTACACT NGG 2 0.0818 Tier II
7 NC_000079.6 13 31950575 - Gmds NNNGCTGCAATACCTAAACA NTG 2 0.0182 Tier II
8 NC_000070.6 4 83564092 - Ccdc171 NNNGCTGCAATAACTACACA NTG 2 0.015 Tier II
9 NC_000081.6 15 95802641 + A130051J06Rik NNNGCTGCAATAGCTACACT NGG 2 0.0818 Tier III
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Other clones with same target sequence:

(none)