Construct: sgRNA BRDN0001148960
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGTACCCAGCAAAAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TWF2 (11344)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77341
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
52231221 |
+ |
TWF2 |
NNNGGTACCCAGCAAAAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
136759245 |
- |
ARHGEF6 |
NNNGGTCTCCAGCAAAAGAG |
NGG |
2 |
0.3828 |
Tier I |
| 3 |
NC_000002.12 |
2 |
74285911 |
+ |
SLC4A5 |
NNNGGGACCCTGCAAAAGAG |
NGG |
2 |
0.1758 |
Tier I |
| 4 |
NC_000017.11 |
17 |
56150017 |
- |
ANKFN1 |
NNNGCTACTCAGCAAAAGAG |
NGG |
2 |
0.6875 |
Tier II |
| 5 |
NC_000012.12 |
12 |
43976353 |
+ |
TMEM117 |
NNNGGTATCCAGTAAAAGAG |
NGG |
2 |
0.6125 |
Tier II |
| 6 |
NC_000008.11 |
8 |
14215943 |
+ |
SGCZ |
NNNGGTACCCAGTGAAAGAG |
NGG |
2 |
0.3267 |
Tier II |
| 7 |
NC_000003.12 |
3 |
76635190 |
- |
ROBO2 |
NNNGGTACCCTTCAAAAGAG |
NGG |
2 |
0.1183 |
Tier II |
| 8 |
NC_000001.11 |
1 |
14951320 |
- |
KAZN |
NNNGGTACCCAGCAGAGGAG |
NGG |
2 |
0.1147 |
Tier II |
| 9 |
NC_000007.14 |
7 |
1875625 |
+ |
MAD1L1 |
NNNGGTTCCCAGCAAAAGAG |
NAG |
2 |
0.1134 |
Tier II |
| 10 |
NC_000003.12 |
3 |
49665083 |
+ |
BSN |
NNNGGTCCCCAGCAAAGGAG |
NGG |
2 |
0.0772 |
Tier II |
| 11 |
NC_000023.11 |
X |
68707900 |
+ |
STARD8 |
NNNGGTACCCAGCCCAAGAG |
NGG |
2 |
0.0584 |
Tier II |
| 12 |
NC_000001.11 |
1 |
54551361 |
+ |
ACOT11 |
NNNGGTACCCAGCAAGTGAG |
NGG |
2 |
0.0256 |
Tier II |
| 13 |
NC_000008.11 |
8 |
26034378 |
- |
EBF2 |
NNNGGTACCCAGCACAAGAG |
NGA |
2 |
0.0189 |
Tier II |
| 14 |
NC_000008.11 |
8 |
58008946 |
- |
FAM110B |
NNNGGTACCCAGAAATAGAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000010.11 |
10 |
48886129 |
- |
WDFY4 |
NNNGGTACCCAGCAACAGAG |
NTG |
2 |
0.0 |
Tier II |
| 16 |
NC_000008.11 |
8 |
92403211 |
+ |
LOC105375638 |
NNNGGGAACCAGCAAAAGAG |
NGG |
2 |
0.3714 |
Tier III |
| 17 |
NC_000014.9 |
14 |
101688507 |
+ |
LINC02320 |
NNNGGGACCCAGCAAAACAG |
NGG |
2 |
0.2721 |
Tier III |
| 18 |
NC_000002.12 |
2 |
19045892 |
- |
LOC105373456 |
NNNGTTATCCAGCAAAAGAG |
NGG |
2 |
0.2625 |
Tier III |
| 19 |
NC_000002.12 |
2 |
191889533 |
- |
CAVIN2-AS1 |
NNNGGTACCCAGGAAAAGAG |
NGG |
1 |
0.1364 |
Tier III |
| 20 |
NC_000020.11 |
20 |
16191044 |
+ |
LOC124904874 |
NNNGGTACCCAGGGAAAGAG |
NGG |
2 |
0.0636 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
105444375 |
- |
Htr1a |
NNNCGTCCCCAGCAAAAGAG |
NGG |
2 |
0.2316 |
Tier I |
| 2 |
NC_000075.6 |
9 |
106212743 |
- |
Twf2 |
NNNGGTACCCAGCCAAGGAG |
NGG |
2 |
0.0378 |
Tier I |
| 3 |
NC_000086.7 |
X |
87807428 |
- |
Il1rapl1 |
NNNAGTACCTAGCAAAAGAG |
NGG |
2 |
0.8471 |
Tier II |
| 4 |
NC_000081.6 |
15 |
98887318 |
+ |
Dhh |
NNNACTACCCAGCAAAAGAG |
NGG |
2 |
0.7071 |
Tier II |
| 5 |
NC_000080.6 |
14 |
34554419 |
- |
Ldb3 |
NNNGGTACCCACCAAAAGAG |
NGG |
1 |
0.5294 |
Tier II |
| 6 |
NC_000082.6 |
16 |
41038033 |
+ |
Lsamp |
NNNGGTATCCACCAAAAGAG |
NGG |
2 |
0.4632 |
Tier II |
| 7 |
NC_000078.6 |
12 |
3648207 |
- |
Dtnb |
NNNGGTGGCCAGCAAAAGAG |
NGG |
2 |
0.4538 |
Tier II |
| 8 |
NC_000079.6 |
13 |
81301303 |
+ |
Adgrv1 |
NNNGGTACCGAGAAAAAGAG |
NGG |
2 |
0.1496 |
Tier II |
| 9 |
NC_000068.7 |
2 |
68321636 |
+ |
Stk39 |
NNNGGTACCCAGCAAAAGAG |
NGA |
1 |
0.0694 |
Tier II |
| 10 |
NC_000071.6 |
5 |
64181775 |
+ |
Tbc1d1 |
NNNGGTACCCAGCAAAGGAG |
NGC |
2 |
0.0039 |
Tier II |
| 11 |
NC_000071.6 |
5 |
100381571 |
+ |
Sec31a |
NNNGGTACCCAGCAACAGAG |
NTG |
2 |
0.0 |
Tier II |
| 12 |
NC_000068.7 |
2 |
119990211 |
- |
Mapkbp1 |
NNNGGTACCCAGCAATAGAG |
NGG |
1 |
0.0 |
Tier II |
| 13 |
NC_000085.6 |
19 |
57335042 |
- |
B230217O12Rik |
NNNACTACCCAGCAAAAGAG |
NGG |
2 |
0.7071 |
Tier III |
| 14 |
NC_000067.6 |
1 |
75949718 |
- |
Gm32622 |
NNNGGTACCCGGCAAAAGAG |
NGG |
1 |
0.65 |
Tier III |
| 15 |
NC_000077.6 |
11 |
55977816 |
- |
LOC115485826 |
NNNGGTACACAGCAGAAGAG |
NGG |
2 |
0.5571 |
Tier III |
| 16 |
NC_000082.6 |
16 |
89133477 |
+ |
Gm41487 |
NNNGATCCCCAGCAAAAGAG |
NGG |
2 |
0.3792 |
Tier III |
| 17 |
NC_000073.6 |
7 |
133218953 |
- |
Gm35567 |
NNNGGAACCCTGCAAAAGAG |
NGG |
2 |
0.2667 |
Tier III |
Other clones with same target sequence:
(none)
