Construct: sgRNA BRDN0001148966
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCACTCTCTCTACGCATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRD2 (6046)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77985
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
32976683 |
- |
BRD2 |
NNNACTCTCTCTACGCATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
51543482 |
- |
PRKG1 |
NNNACTCTCTCTACTCATAG |
NGT |
2 |
0.0023 |
Tier II |
| 3 |
NC_000018.10 |
18 |
39884262 |
+ |
LINC01902 |
NNNACCCTCTCTACACATAG |
NGG |
2 |
0.8556 |
Tier III |
| 4 |
NC_000018.10 |
18 |
39884262 |
+ |
LINC01901 |
NNNACCCTCTCTACACATAG |
NGG |
2 |
0.8556 |
Tier III |
| 5 |
NC_000018.10 |
18 |
76298355 |
- |
LOC105372211 |
NNNAGTTTCTCTACGCATAG |
NGG |
2 |
0.4875 |
Tier III |
| 6 |
NC_000002.12 |
2 |
168254707 |
+ |
LOC107985959 |
NNNACTATCTCTACCCATAG |
NGG |
2 |
0.2045 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
34115144 |
+ |
Brd2 |
NNNACTCTCTCTGCGCATAG |
NAG |
2 |
0.1691 |
Tier I |
| 2 |
NC_000068.7 |
2 |
22399238 |
+ |
Myo3a |
NNNACTCTCTCTAAGCATAA |
NGG |
2 |
0.3281 |
Tier II |
| 3 |
NC_000077.6 |
11 |
119027721 |
+ |
Cbx2 |
NNNATTCTCTCTACGCACAG |
NGG |
2 |
0.2727 |
Tier II |
| 4 |
NC_000083.6 |
17 |
84547558 |
- |
Plekhh2 |
NNNACTCTTTGTACGCATAG |
NGG |
2 |
0.2188 |
Tier II |
| 5 |
NC_000083.6 |
17 |
84547636 |
- |
Plekhh2 |
NNNACTCTTTGTACGCATAG |
NGG |
2 |
0.2188 |
Tier II |
| 6 |
NC_000083.6 |
17 |
84547558 |
- |
4930500A05Rik |
NNNACTCTTTGTACGCATAG |
NGG |
2 |
0.2188 |
Tier III |
| 7 |
NC_000083.6 |
17 |
84547636 |
- |
4930500A05Rik |
NNNACTCTTTGTACGCATAG |
NGG |
2 |
0.2188 |
Tier III |
| 8 |
NC_000068.7 |
2 |
95520833 |
- |
Gm13794 |
NNNACTCTCTCTACACTTAG |
NGG |
2 |
0.1255 |
Tier III |
Other clones with same target sequence:
(none)
