Construct: sgRNA BRDN0001148992

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACGGAGACTCTGGCACATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPR1 (4881)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77303

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 153681778 + NPR1 NNNGAGACTCTGGCACATGG NGG 0 1.0 Tier I
2 NC_000011.10 11 57494095 + SLC43A1 NNNGAGCCTCTGGCCCATGG NGG 2 0.1193 Tier I
3 NC_000006.12 6 30603623 - PPP1R10 NNNGAGACTCTGGAACCTGG NGG 2 0.0618 Tier I
4 NC_000001.11 1 3869672 - DFFB NNNGAGCCTCTGGCACATGG NGC 2 0.0097 Tier I
5 NC_000005.10 5 9446470 - SEMA5A NNNGAAAATCTGGCACATGG NGG 2 0.65 Tier II
6 NC_000010.11 10 12947489 - CCDC3 NNNAACACTCTGGCACATGG NGG 2 0.6136 Tier II
7 NC_000014.9 14 100579324 + BEGAIN NNNGAGCCTTTGGCACATGG NGG 2 0.4118 Tier II
8 NC_000015.10 15 100285947 - ADAMTS17 NNNGAGAGGCTGGCACATGG NGG 2 0.398 Tier II
9 NC_000002.12 2 204758122 - PARD3B NNNGAGACACTGCCACATGG NGG 2 0.391 Tier II
10 NC_000005.10 5 112897473 + SRP19 NNNCAGAATCTGGCACATGG NGG 2 0.3441 Tier II
11 NC_000005.10 5 112897473 + REEP5 NNNCAGAATCTGGCACATGG NGG 2 0.3441 Tier II
12 NC_000012.12 12 104263160 - TXNRD1 NNNGAGCCTCTGGCACAAGG NGG 2 0.2917 Tier II
13 NC_000006.12 6 32329291 + TSBP1 NNNGATTCTCTGGCACATGG NGG 2 0.2917 Tier II
14 NC_000011.10 11 812419 + RPLP2 NNNGAGACACTGGCATATGG NGG 2 0.2857 Tier II
15 NC_000001.11 1 111815341 - KCND3 NNNGAGACTCTGTCACATGA NGG 2 0.2813 Tier II
16 NC_000017.11 17 16224674 - PIGL NNNGAGACTCTGTCACAAGG NGG 2 0.2 Tier II
17 NC_000019.10 19 43361386 + CD177 NNNGAGACTCTGGCCCAAGG NGG 2 0.1818 Tier II
18 NC_000001.11 1 242465930 + PLD5 NNNAAGACTCTGGCTCATGG NGG 2 0.18 Tier II
19 NC_000003.12 3 38093021 - DLEC1 NNNGAGACTCTGGCACCTGG NGG 1 0.1765 Tier II
20 NC_000019.10 19 9838301 + PIN1 NNNGAGCCTGTGGCACATGG NGG 2 0.1701 Tier II
21 NC_000014.9 14 104599192 - TMEM179 NNNGAGACACTGGCACATGG NGA 2 0.0645 Tier II
22 NC_000017.11 17 39317249 - FBXL20 NNNGAGACTCTGTCTCATGG NGG 2 0.06 Tier II
23 NC_000021.9 21 41309385 - FAM3B NNNGAGACCCTGGCACATGG NTG 2 0.036 Tier II
24 NC_000004.12 4 19607431 - LOC105374511 NNNAAGACCCTGGCACATGG NGG 2 0.8308 Tier III
25 NC_000010.11 10 91395799 + HECTD2-AS1 NNNGAGATTATGGCACATGG NGG 2 0.7583 Tier III
26 NC_000021.9 21 41692320 + LINC00111 NNNGAGACTCTGGCACAGGG NGG 1 0.3333 Tier III
27 NC_000012.12 12 104263160 - LOC124903002 NNNGAGCCTCTGGCACAAGG NGG 2 0.2917 Tier III
28 NC_000006.12 6 32329291 + TSBP1-AS1 NNNGATTCTCTGGCACATGG NGG 2 0.2917 Tier III
29 NC_000005.10 5 152357650 + LOC105378237 NNNGAGATTCTGTCACATGG NGG 2 0.2625 Tier III
30 NC_000011.10 11 76300320 - LOC105369395 NNNGAGACTCTGGCACAGGT NGG 2 0.2333 Tier III
31 NC_000019.10 19 43373395 - CD177P1 NNNGAGACTCTGGCCCAAGG NGG 2 0.1818 Tier III
32 NC_000013.11 13 44895519 + LOC105370187 NNNCAGACTCTGGCACATGG NAG 2 0.1373 Tier III
33 NC_000013.11 13 59117051 - LOC105370224 NNNCAGACTCTGGCACATGG NAG 2 0.1373 Tier III
34 NC_000014.9 14 97686942 + LOC105370650 NNNGAGCCTCTGTCACATGG NGG 2 0.1313 Tier III
35 NC_000002.12 2 9769344 - LOC100996549 NNNGAGTCTCTGGCACCTGG NGG 2 0.0772 Tier III
36 NC_000016.10 16 66752036 - DYNC1LI2-DT NNNGTGACTCTGGCACATGG NGA 2 0.0253 Tier III
37 NC_000014.9 14 39594966 + LOC105370461 NNNGAGATTCTGGCACATGG NGT 2 0.0141 Tier III
38 NC_000005.10 5 171485648 + LOC105377721 NNNGAGACTCTGGGACATGT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 90462630 - Npr1 NNNGAGACTCTGGCACAGGG NGG 1 0.3333 Tier I
2 NC_000068.7 2 127292169 - Stard7 NNNGAGACTCTGGCACACTG NGG 2 0.2857 Tier I
3 NC_000077.6 11 89620908 + Ankfn1 NNNGAAACTCTAGCACATGG NGG 2 0.9333 Tier II
4 NC_000080.6 14 16491444 - Rarb NNNAAGACTCTGGCACATGG NGG 1 0.9 Tier II
5 NC_000067.6 1 193260985 - Hsd11b1 NNNGAGACCTTGGCACATGG NGG 2 0.8688 Tier II
6 NC_000073.6 7 65991616 + Pcsk6 NNNGAGACTCAGGCACATGA NGG 2 0.7031 Tier II
7 NC_000075.6 9 69042753 + Rora NNNGAGACTCAGGCACATGA NGG 2 0.7031 Tier II
8 NC_000069.6 3 96163869 - Mtmr11 NNNGAGACCCTGGCGCATGG NGG 2 0.6 Tier II
9 NC_000079.6 13 34064529 - Bphl NNNGGGACTCAGGCACATGG NGG 2 0.54 Tier II
10 NC_000071.6 5 130886311 - Galnt17 NNNGAGACTCAGGCACATGT NGG 2 0.525 Tier II
11 NC_000072.6 6 123003516 + Clec4a4 NNNGAGAGTCTGGTACATGG NGG 2 0.4714 Tier II
12 NC_000072.6 6 15792760 - Mdfic NNNGAGCCTCTGGCACATGG NGG 1 0.4375 Tier II
13 NC_000080.6 14 64260708 + Msra NNNGAAACTGTGGCACATGG NGG 2 0.3889 Tier II
14 NC_000071.6 5 118226730 - Rnft2 NNNGAGACTCTGGCACAGGG NGG 1 0.3333 Tier II
15 NC_000067.6 1 58404159 - Bzw1 NNNGCTACTCTGGCACATGG NGG 2 0.3333 Tier II
16 NC_000076.6 10 93275190 - Elk3 NNNGAGTCTCAGGCACATGG NGG 2 0.3281 Tier II
17 NC_000072.6 6 113368801 + Tada3 NNNGAGACTCTGGCACACAG NGG 2 0.3061 Tier II
18 NC_000079.6 13 48961442 + Fam120a NNNGAGACTGTGGCACATGT NGG 2 0.2722 Tier II
19 NC_000074.6 8 95800660 - Cnot1 NNNGAGACGCTGCCACATGG NGG 2 0.2607 Tier II
20 NC_000073.6 7 30315648 - Syne4 NNNGAGACTCTGTCACATAG NGG 2 0.2143 Tier II
21 NC_000068.7 2 14434841 - Slc39a12 NNNCAGACTCTTGCACATGG NGG 2 0.2036 Tier II
22 NC_000068.7 2 131146654 + 1700037H04Rik NNNGAGACTCAGGCACATGG NAG 2 0.1944 Tier II
23 NC_000067.6 1 107517667 - Serpinb2 NNNGTGACTCTGGCACATGC NGG 2 0.1558 Tier II
24 NC_000084.6 18 56872384 + Marchf3 NNNGAGACTGGGGCACATGG NGG 2 0.1556 Tier II
25 NC_000068.7 2 156558004 + Aar2 NNNGAAACTCTGGCACTTGG NGG 2 0.1333 Tier II
26 NC_000073.6 7 81672378 + Homer2 NNNGAGATTCTGGCACTTGG NGG 2 0.1167 Tier II
27 NC_000085.6 19 24816795 - Pgm5 NNNGAGCCTCTGGCACATGG NAG 2 0.1134 Tier II
28 NC_000070.6 4 128281385 + Csmd2 NNNGAGACTCTGGCACATGC NAG 2 0.1111 Tier II
29 NC_000068.7 2 121315794 - Ppip5k1 NNNGTGACTCTGGCCCATGG NGG 2 0.0992 Tier II
30 NC_000079.6 13 33901259 + Serpinb6c NNNGACACTCTGGCACTTGG NGG 2 0.0909 Tier II
31 NC_000080.6 14 14950797 - Nek10 NNNGAGACTGTGGCAGATGG NGG 2 0.0598 Tier II
32 NC_000070.6 4 47040529 + Anks6 NNNGAGACTCTGGCACTTGC NGG 2 0.0571 Tier II
33 NC_000081.6 15 83933689 + Efcab6 NNNGAGGCTCTGGCACATGG NGA 2 0.049 Tier II
34 NC_000086.7 X 56568896 - Gm648 NNNGAGGCTCTGGCACATGG NTG 2 0.0275 Tier II
35 NC_000068.7 2 30248494 - Lrrc8a NNNGAGGCTCTGGCACATGG NGT 2 0.0114 Tier II
36 NC_000073.6 7 84270917 - Arnt2 NNNGAGCCTCTGGGACATGG NGG 2 0.0 Tier II
37 NC_000080.6 14 64260708 + Gm41176 NNNGAAACTGTGGCACATGG NGG 2 0.3889 Tier III
38 NC_000075.6 9 45419824 + 4833428L15Rik NNNGAGACTCTGGCACACTG NGG 2 0.2857 Tier III
39 NC_000083.6 17 36217922 - Gm5682 NNNGAGACTCTGGCTCATGG NGG 1 0.2 Tier III
40 NC_000071.6 5 42128832 + Gm16223 NNNGTGACTCTGCCACATGG NGG 2 0.1531 Tier III
41 NC_000070.6 4 108393842 - Gm32297 NNNGAGACTCTGTCCCATGG NGG 2 0.0818 Tier III
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Other clones with same target sequence:

(none)