Construct: sgRNA BRDN0001149026
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCGGCTGAAGTCTAAGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNIK (23043)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75851
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
171175257 |
- |
TNIK |
NNNGGCTGAAGTCTAAGAAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000019.10 |
19 |
1055920 |
+ |
ABCA7 |
NNNGCCTGAAGACTAAGAAG |
NGG |
2 |
0.6286 |
Tier I |
3 |
NC_000017.11 |
17 |
4886194 |
+ |
MINK1 |
NNNGGCTCAAGTCCAAGAAG |
NGG |
2 |
0.1758 |
Tier I |
4 |
NC_000012.12 |
12 |
44098631 |
- |
TMEM117 |
NNNGGCAGAAGTCTAAGAAG |
NGG |
1 |
0.875 |
Tier II |
5 |
NC_000002.12 |
2 |
216319685 |
- |
MARCHF4 |
NNNGCCTGAAGTCAAAGAAG |
NGG |
2 |
0.4864 |
Tier II |
6 |
NC_000015.10 |
15 |
100983748 |
- |
LRRK1 |
NNNGGGTGAAGCCTAAGAAG |
NGG |
2 |
0.4737 |
Tier II |
7 |
NC_000007.14 |
7 |
133366264 |
- |
EXOC4 |
NNNGGCAGAAGGCTAAGAAG |
NGG |
2 |
0.4375 |
Tier II |
8 |
NC_000003.12 |
3 |
133367739 |
- |
TMEM108 |
NNNGGCTGATGTCTAAGGAG |
NGG |
2 |
0.3529 |
Tier II |
9 |
NC_000016.10 |
16 |
24015893 |
+ |
PRKCB |
NNNGACTGAAGTCCAAGAAG |
NGG |
2 |
0.2476 |
Tier II |
10 |
NC_000004.12 |
4 |
5988073 |
+ |
C4orf50 |
NNNTGCTGGAGTCTAAGAAG |
NGG |
2 |
0.2424 |
Tier II |
11 |
NC_000001.11 |
1 |
93590729 |
- |
BCAR3 |
NNNGGCTCAATTCTAAGAAG |
NGG |
2 |
0.2367 |
Tier II |
12 |
NC_000003.12 |
3 |
65940379 |
+ |
MAGI1 |
NNNGGCTGAAGTCTAAGATC |
NGG |
2 |
0.2308 |
Tier II |
13 |
NC_000004.12 |
4 |
93298323 |
+ |
GRID2 |
NNNGGCTGAAGTCTAAGATC |
NGG |
2 |
0.2308 |
Tier II |
14 |
NC_000017.11 |
17 |
40452287 |
+ |
IGFBP4 |
NNNGTCTGAAGTCTAAGAAT |
NGG |
2 |
0.21 |
Tier II |
15 |
NC_000005.10 |
5 |
22728676 |
+ |
CDH12 |
NNNTGCTGAAGTCTAAGATG |
NGG |
2 |
0.1958 |
Tier II |
16 |
NC_000009.12 |
9 |
99152269 |
- |
TGFBR1 |
NNNGGCTGAAGTCAAAGCAG |
NGG |
2 |
0.1179 |
Tier II |
17 |
NC_000008.11 |
8 |
42293134 |
- |
IKBKB |
NNNGGCTGAAGTCTAAGCAC |
NGG |
2 |
0.0816 |
Tier II |
18 |
NC_000010.11 |
10 |
103578528 |
- |
NEURL1 |
NNNGGCTGAAGTCTAGGAGG |
NGG |
2 |
0.0721 |
Tier II |
19 |
NC_000002.12 |
2 |
178994293 |
+ |
CCDC141 |
NNNTGCTGAAGTCTAGGAAG |
NGG |
2 |
0.0699 |
Tier II |
20 |
NC_000015.10 |
15 |
95480958 |
+ |
LINC00924 |
NNNGAATGAAGTCTAAGAAG |
NGG |
2 |
0.8048 |
Tier III |
21 |
NC_000015.10 |
15 |
95480958 |
+ |
LOC105370993 |
NNNGAATGAAGTCTAAGAAG |
NGG |
2 |
0.8048 |
Tier III |
22 |
NC_000002.12 |
2 |
216319685 |
- |
LOC107985983 |
NNNGCCTGAAGTCAAAGAAG |
NGG |
2 |
0.4864 |
Tier III |
23 |
NC_000003.12 |
3 |
133367739 |
- |
LOC101927432 |
NNNGGCTGATGTCTAAGGAG |
NGG |
2 |
0.3529 |
Tier III |
24 |
NC_000004.12 |
4 |
107282624 |
- |
LOC102725220 |
NNNAGCTGAAGTATAAGAAG |
NGG |
2 |
0.3462 |
Tier III |
25 |
NC_000003.12 |
3 |
65940379 |
+ |
MAGI1-IT1 |
NNNGGCTGAAGTCTAAGATC |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000069.6 |
3 |
28557113 |
+ |
Tnik |
NNNGGCTCAAGTCTAAGAAG |
NGG |
1 |
0.6154 |
Tier I |
2 |
NC_000071.6 |
5 |
113092452 |
- |
2900026A02Rik |
NNNAGCTGAAGTCCAAGAAG |
NGG |
2 |
0.2571 |
Tier I |
3 |
NC_000077.6 |
11 |
65844364 |
- |
Dnah9 |
NNNGGCTGATGACTAAGAAG |
NGG |
2 |
0.7059 |
Tier II |
4 |
NC_000086.7 |
X |
25630807 |
+ |
Gm2003 |
NNNGGCTGAAATCAAAGAAG |
NGG |
2 |
0.619 |
Tier II |
5 |
NC_000086.7 |
X |
26531066 |
+ |
Slx |
NNNGGCTGAAATCAAAGAAG |
NGG |
2 |
0.619 |
Tier II |
6 |
NC_000085.6 |
19 |
57000962 |
+ |
Afap1l2 |
NNNAGCTGAAGTCTGAGAAG |
NGG |
2 |
0.585 |
Tier II |
7 |
NC_000075.6 |
9 |
10527464 |
+ |
Cntn5 |
NNNGGCTAAGGTCTAAGAAG |
NGG |
2 |
0.5556 |
Tier II |
8 |
NC_000075.6 |
9 |
50842153 |
- |
Alg9 |
NNNGGCTGAAGTCTGAGAAT |
NGG |
2 |
0.455 |
Tier II |
9 |
NC_000070.6 |
4 |
108600747 |
+ |
Orc1 |
NNNGGCTGAAATCTAAGAAC |
NGG |
2 |
0.4286 |
Tier II |
10 |
NC_000073.6 |
7 |
90852294 |
- |
Dlg2 |
NNNGGCTTCAGTCTAAGAAG |
NGG |
2 |
0.3571 |
Tier II |
11 |
NC_000070.6 |
4 |
57517542 |
- |
Pakap |
NNNGGCTGTGGTCTAAGAAG |
NGG |
2 |
0.3333 |
Tier II |
12 |
NC_000067.6 |
1 |
132321550 |
- |
Nuak2 |
NNNGGCTGAAGCCTAAGAAG |
NAG |
2 |
0.2456 |
Tier II |
13 |
NC_000085.6 |
19 |
53614240 |
+ |
Smc3 |
NNNGGCTGTAGTCTAAGGAG |
NGG |
2 |
0.24 |
Tier II |
14 |
NC_000076.6 |
10 |
80230661 |
- |
Pwwp3a |
NNNGGCAGAAGTCTAAGAAG |
NAG |
2 |
0.2269 |
Tier II |
15 |
NC_000078.6 |
12 |
84835186 |
- |
Ltbp2 |
NNNGCCTGAAGTCTAAGCAG |
NGG |
2 |
0.1497 |
Tier II |
16 |
NC_000080.6 |
14 |
57351694 |
+ |
Cryl1 |
NNNGGCTGAAGTCTGAGCAG |
NGG |
2 |
0.1238 |
Tier II |
17 |
NC_000067.6 |
1 |
176043982 |
+ |
Pld5 |
NNNGGCAGAAGTCTAAGAAG |
NCG |
2 |
0.0937 |
Tier II |
18 |
NC_000076.6 |
10 |
71090103 |
+ |
Bicc1 |
NNNGGCTGAAGTCTCAGCAG |
NGG |
2 |
0.0519 |
Tier II |
19 |
NC_000086.7 |
X |
69858284 |
+ |
Aff2 |
NNNGGCTGAAGTCTAAGAAT |
NTG |
2 |
0.0273 |
Tier II |
20 |
NC_000069.6 |
3 |
30379342 |
- |
Mecom |
NNNGGCTGAAGTCTAAGGAG |
NGT |
2 |
0.0065 |
Tier II |
21 |
NC_000075.6 |
9 |
8915373 |
+ |
Pgr |
NNNGGCTGAAGTCTATAAAG |
NGG |
2 |
0.0 |
Tier II |
22 |
NC_000067.6 |
1 |
58290196 |
- |
Aox2 |
NNNGTCTGAAGTCTATGAAG |
NGG |
2 |
0.0 |
Tier II |
23 |
NC_000078.6 |
12 |
113991542 |
- |
Igh |
NNNGGCTGAAATCAAAGAAG |
NGG |
2 |
0.619 |
Tier III |
24 |
NC_000070.6 |
4 |
108600747 |
+ |
Gm12743 |
NNNGGCTGAAATCTAAGAAC |
NGG |
2 |
0.4286 |
Tier III |
25 |
NC_000072.6 |
6 |
30319397 |
+ |
1700095J07Rik |
NNNGGCTGAACTCTAAGAAG |
NGG |
1 |
0.4286 |
Tier III |
26 |
NC_000080.6 |
14 |
61493801 |
+ |
4930556J02Rik |
NNNGGCTGAAGTCTAAGAAC |
NGG |
1 |
0.4286 |
Tier III |
27 |
NC_000067.6 |
1 |
132321550 |
- |
Gm30744 |
NNNGGCTGAAGCCTAAGAAG |
NAG |
2 |
0.2456 |
Tier III |
28 |
NC_000080.6 |
14 |
76164989 |
+ |
Gm36534 |
NNNGTCTGAGGTCTAAGAAG |
NGG |
2 |
0.1667 |
Tier III |
29 |
NC_000072.6 |
6 |
48875713 |
+ |
Gm44764 |
NNNTGCTGAAGTCTAAGAGG |
NGG |
2 |
0.1364 |
Tier III |
30 |
NC_000068.7 |
2 |
68841148 |
- |
Gm36231 |
NNNGTCTGAAGTCTCAGAAG |
NGG |
2 |
0.0818 |
Tier III |
31 |
NC_000075.6 |
9 |
61050700 |
- |
Gm33699 |
NNNGGCTGAAGTCTCAGAAG |
NGA |
2 |
0.0189 |
Tier III |
32 |
NC_000067.6 |
1 |
58290196 |
- |
Gm15759 |
NNNGTCTGAAGTCTATGAAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)