Construct: sgRNA BRDN0001149026

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCGGCTGAAGTCTAAGAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNIK (23043)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75851

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 171175257 - TNIK NNNGGCTGAAGTCTAAGAAG NGG 0 1.0 Tier I
2 NC_000019.10 19 1055920 + ABCA7 NNNGCCTGAAGACTAAGAAG NGG 2 0.6286 Tier I
3 NC_000017.11 17 4886194 + MINK1 NNNGGCTCAAGTCCAAGAAG NGG 2 0.1758 Tier I
4 NC_000012.12 12 44098631 - TMEM117 NNNGGCAGAAGTCTAAGAAG NGG 1 0.875 Tier II
5 NC_000002.12 2 216319685 - MARCHF4 NNNGCCTGAAGTCAAAGAAG NGG 2 0.4864 Tier II
6 NC_000015.10 15 100983748 - LRRK1 NNNGGGTGAAGCCTAAGAAG NGG 2 0.4737 Tier II
7 NC_000007.14 7 133366264 - EXOC4 NNNGGCAGAAGGCTAAGAAG NGG 2 0.4375 Tier II
8 NC_000003.12 3 133367739 - TMEM108 NNNGGCTGATGTCTAAGGAG NGG 2 0.3529 Tier II
9 NC_000016.10 16 24015893 + PRKCB NNNGACTGAAGTCCAAGAAG NGG 2 0.2476 Tier II
10 NC_000004.12 4 5988073 + C4orf50 NNNTGCTGGAGTCTAAGAAG NGG 2 0.2424 Tier II
11 NC_000001.11 1 93590729 - BCAR3 NNNGGCTCAATTCTAAGAAG NGG 2 0.2367 Tier II
12 NC_000003.12 3 65940379 + MAGI1 NNNGGCTGAAGTCTAAGATC NGG 2 0.2308 Tier II
13 NC_000004.12 4 93298323 + GRID2 NNNGGCTGAAGTCTAAGATC NGG 2 0.2308 Tier II
14 NC_000017.11 17 40452287 + IGFBP4 NNNGTCTGAAGTCTAAGAAT NGG 2 0.21 Tier II
15 NC_000005.10 5 22728676 + CDH12 NNNTGCTGAAGTCTAAGATG NGG 2 0.1958 Tier II
16 NC_000009.12 9 99152269 - TGFBR1 NNNGGCTGAAGTCAAAGCAG NGG 2 0.1179 Tier II
17 NC_000008.11 8 42293134 - IKBKB NNNGGCTGAAGTCTAAGCAC NGG 2 0.0816 Tier II
18 NC_000010.11 10 103578528 - NEURL1 NNNGGCTGAAGTCTAGGAGG NGG 2 0.0721 Tier II
19 NC_000002.12 2 178994293 + CCDC141 NNNTGCTGAAGTCTAGGAAG NGG 2 0.0699 Tier II
20 NC_000015.10 15 95480958 + LINC00924 NNNGAATGAAGTCTAAGAAG NGG 2 0.8048 Tier III
21 NC_000015.10 15 95480958 + LOC105370993 NNNGAATGAAGTCTAAGAAG NGG 2 0.8048 Tier III
22 NC_000002.12 2 216319685 - MARCHF4-AS1 NNNGCCTGAAGTCAAAGAAG NGG 2 0.4864 Tier III
23 NC_000003.12 3 133367739 - LOC101927432 NNNGGCTGATGTCTAAGGAG NGG 2 0.3529 Tier III
24 NC_000004.12 4 107282624 - LOC102725220 NNNAGCTGAAGTATAAGAAG NGG 2 0.3462 Tier III
25 NC_000003.12 3 65940379 + MAGI1-IT1 NNNGGCTGAAGTCTAAGATC NGG 2 0.2308 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 28557113 + Tnik NNNGGCTCAAGTCTAAGAAG NGG 1 0.6154 Tier I
2 NC_000071.6 5 113092452 - 2900026A02Rik NNNAGCTGAAGTCCAAGAAG NGG 2 0.2571 Tier I
3 NC_000077.6 11 65844364 - Dnah9 NNNGGCTGATGACTAAGAAG NGG 2 0.7059 Tier II
4 NC_000086.7 X 25630807 + Gm2003 NNNGGCTGAAATCAAAGAAG NGG 2 0.619 Tier II
5 NC_000086.7 X 26531066 + Slx NNNGGCTGAAATCAAAGAAG NGG 2 0.619 Tier II
6 NC_000085.6 19 57000962 + Afap1l2 NNNAGCTGAAGTCTGAGAAG NGG 2 0.585 Tier II
7 NC_000075.6 9 10527464 + Cntn5 NNNGGCTAAGGTCTAAGAAG NGG 2 0.5556 Tier II
8 NC_000075.6 9 50842153 - Alg9 NNNGGCTGAAGTCTGAGAAT NGG 2 0.455 Tier II
9 NC_000070.6 4 108600747 + Orc1 NNNGGCTGAAATCTAAGAAC NGG 2 0.4286 Tier II
10 NC_000073.6 7 90852294 - Dlg2 NNNGGCTTCAGTCTAAGAAG NGG 2 0.3571 Tier II
11 NC_000070.6 4 57517542 - Pakap NNNGGCTGTGGTCTAAGAAG NGG 2 0.3333 Tier II
12 NC_000067.6 1 132321550 - Nuak2 NNNGGCTGAAGCCTAAGAAG NAG 2 0.2456 Tier II
13 NC_000085.6 19 53614240 + Smc3 NNNGGCTGTAGTCTAAGGAG NGG 2 0.24 Tier II
14 NC_000076.6 10 80230661 - Pwwp3a NNNGGCAGAAGTCTAAGAAG NAG 2 0.2269 Tier II
15 NC_000078.6 12 84835186 - Ltbp2 NNNGCCTGAAGTCTAAGCAG NGG 2 0.1497 Tier II
16 NC_000080.6 14 57351694 + Cryl1 NNNGGCTGAAGTCTGAGCAG NGG 2 0.1238 Tier II
17 NC_000067.6 1 176043982 + Pld5 NNNGGCAGAAGTCTAAGAAG NCG 2 0.0937 Tier II
18 NC_000076.6 10 71090103 + Bicc1 NNNGGCTGAAGTCTCAGCAG NGG 2 0.0519 Tier II
19 NC_000086.7 X 69858284 + Aff2 NNNGGCTGAAGTCTAAGAAT NTG 2 0.0273 Tier II
20 NC_000069.6 3 30379342 - Mecom NNNGGCTGAAGTCTAAGGAG NGT 2 0.0065 Tier II
21 NC_000075.6 9 8915373 + Pgr NNNGGCTGAAGTCTATAAAG NGG 2 0.0 Tier II
22 NC_000067.6 1 58290196 - Aox2 NNNGTCTGAAGTCTATGAAG NGG 2 0.0 Tier II
23 NC_000078.6 12 113991542 - Igh NNNGGCTGAAATCAAAGAAG NGG 2 0.619 Tier III
24 NC_000070.6 4 108600747 + Gm12743 NNNGGCTGAAATCTAAGAAC NGG 2 0.4286 Tier III
25 NC_000072.6 6 30319397 + 1700095J07Rik NNNGGCTGAACTCTAAGAAG NGG 1 0.4286 Tier III
26 NC_000080.6 14 61493801 + 4930556J02Rik NNNGGCTGAAGTCTAAGAAC NGG 1 0.4286 Tier III
27 NC_000067.6 1 132321550 - Gm30744 NNNGGCTGAAGCCTAAGAAG NAG 2 0.2456 Tier III
28 NC_000080.6 14 76164989 + Gm36534 NNNGTCTGAGGTCTAAGAAG NGG 2 0.1667 Tier III
29 NC_000072.6 6 48875713 + Gm44764 NNNTGCTGAAGTCTAAGAGG NGG 2 0.1364 Tier III
30 NC_000068.7 2 68841148 - Gm36231 NNNGTCTGAAGTCTCAGAAG NGG 2 0.0818 Tier III
31 NC_000075.6 9 61050700 - Gm33699 NNNGGCTGAAGTCTCAGAAG NGA 2 0.0189 Tier III
32 NC_000067.6 1 58290196 - Gm15759 NNNGTCTGAAGTCTATGAAG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)

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