Construct: sgRNA BRDN0001149035
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCAGGGCCAGCATCCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PASK (23178)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76204
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
241126364 |
+ |
PASK |
NNNAGGGCCAGCATCCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
41620616 |
- |
CEACAM4 |
NNNAGGGCCAGCATCCAGCG |
NGA |
2 |
0.0278 |
Tier I |
| 3 |
NC_000013.11 |
13 |
25940812 |
- |
ATP8A2 |
NNNAGAGCCAGCATCCAACA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000003.12 |
3 |
57762632 |
- |
SLMAP |
NNNAGGGCCAGCATCCAACA |
NGG |
1 |
0.9375 |
Tier II |
| 5 |
NC_000008.11 |
8 |
86594152 |
+ |
CNGB3 |
NNNGGGGACAGCATCCAACG |
NGG |
2 |
0.4063 |
Tier II |
| 6 |
NC_000011.10 |
11 |
21506153 |
+ |
NELL1 |
NNNAAGGCCAGCATCCAATG |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000007.14 |
7 |
4107639 |
+ |
SDK1 |
NNNAGGGCCAGCATTCAACA |
NGG |
2 |
0.0625 |
Tier II |
| 8 |
NC_000022.11 |
22 |
38827083 |
- |
NPTXR |
NNNAGGGCCAGCATCCTAGG |
NGG |
2 |
0.0167 |
Tier II |
| 9 |
NC_000014.9 |
14 |
79426063 |
+ |
NRXN3 |
NNNAGGGCCAGCATCCAAGG |
NTG |
2 |
0.0049 |
Tier II |
| 10 |
NC_000008.11 |
8 |
86594152 |
+ |
MIOXP1 |
NNNGGGGACAGCATCCAACG |
NGG |
2 |
0.4063 |
Tier III |
| 11 |
NC_000002.12 |
2 |
237955642 |
- |
LOC124908001 |
NNNAGGGCCAGCCTCCAAGG |
NGG |
2 |
0.0263 |
Tier III |
| 12 |
NC_000010.11 |
10 |
50669711 |
+ |
LOC105378302 |
NNNAGGGCCAGCACCCAACG |
NGC |
2 |
0.0063 |
Tier III |
| 13 |
NC_000008.11 |
8 |
94026369 |
- |
LOC124901979 |
NNNAGGGCCAGCATCCAACG |
NCA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
101317954 |
+ |
Nlgn3 |
NNNAGGGCCATCATCCAAAG |
NGG |
2 |
0.1648 |
Tier I |
| 2 |
NC_000073.6 |
7 |
78356132 |
+ |
Ntrk3 |
NNNAGGCCCAGCATCCAACG |
NTG |
2 |
0.0268 |
Tier I |
| 3 |
NC_000081.6 |
15 |
79923720 |
+ |
Cbx7 |
NNNAGAGCCAGCATCCAACT |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000073.6 |
7 |
116394652 |
+ |
Pik3c2a |
NNNAGGGCCAGCATCAAATG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000069.6 |
3 |
114951259 |
- |
Olfm3 |
NNNAGGGGCAGCATCCAACT |
NGG |
2 |
0.45 |
Tier II |
| 6 |
NC_000076.6 |
10 |
58812197 |
- |
Sh3rf3 |
NNNAGGGCAAGCATCCAAAG |
NGG |
2 |
0.3673 |
Tier II |
| 7 |
NC_000071.6 |
5 |
132373750 |
+ |
Auts2 |
NNNATGGCCAGCATCCAACT |
NGG |
2 |
0.21 |
Tier II |
| 8 |
NC_000075.6 |
9 |
64199421 |
+ |
Map2k1 |
NNNAGGGCCAGCATACAACA |
NGG |
2 |
0.2083 |
Tier II |
| 9 |
NC_000084.6 |
18 |
75946884 |
+ |
Zbtb7c |
NNNAGGGCGAGCTTCCAACG |
NGG |
2 |
0.1857 |
Tier II |
| 10 |
NC_000068.7 |
2 |
118700924 |
+ |
Ankrd63 |
NNNTGGGCCAGCATCCCACG |
NGG |
2 |
0.1123 |
Tier II |
| 11 |
NC_000082.6 |
16 |
94521689 |
- |
Vps26c |
NNNAGGGTCAGCATCCAAGG |
NGG |
2 |
0.1094 |
Tier II |
| 12 |
NC_000070.6 |
4 |
120267636 |
+ |
Foxo6 |
NNNAGGGCCAGCATCCACAG |
NGG |
2 |
0.0816 |
Tier II |
| 13 |
NC_000076.6 |
10 |
22662330 |
- |
Slc2a12 |
NNNAGGGCCAGGATCGAACG |
NGG |
2 |
0.0684 |
Tier II |
| 14 |
NC_000071.6 |
5 |
111269206 |
- |
Ttc28 |
NNNAGGGCCAGCATCTAAGG |
NGG |
2 |
0.0385 |
Tier II |
| 15 |
NC_000074.6 |
8 |
72727903 |
- |
Sin3b |
NNNAGGGCCAGCATCCAAGG |
NCG |
2 |
0.0134 |
Tier II |
| 16 |
NC_000082.6 |
16 |
94521689 |
- |
Gm31323 |
NNNAGGGTCAGCATCCAAGG |
NGG |
2 |
0.1094 |
Tier III |
| 17 |
NC_000081.6 |
15 |
25291520 |
+ |
4930445E18Rik |
NNNAGGGCCAGCAGCAAACG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000069.6 |
3 |
131919713 |
- |
Gm29865 |
NNNAGGGCCAGCAGCCAACC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
