Construct: sgRNA BRDN0001149057
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGAGTTGGTGTATGGCTTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PSKH1 (5681)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000016.10 | 16 | 67909565 | - | PSKH1 | NNNAGTTGGTGTATGGCTTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 215766782 | + | USH2A | NNNAGTTGGTGTATGGCTGG | NGA | 2 | 0.0174 | Tier I |
3 | NC_000017.11 | 17 | 33369938 | - | ASIC2 | NNNAGTTGGAGTATGGCTTA | NGG | 2 | 0.8036 | Tier II |
4 | NC_000003.12 | 3 | 58069910 | + | FLNB | NNNACTTGGAGTATGGCTTG | NGG | 2 | 0.6735 | Tier II |
5 | NC_000020.11 | 20 | 31710756 | - | BCL2L1 | NNNACTTGGTGAATGGCTTG | NGG | 2 | 0.6286 | Tier II |
6 | NC_000011.10 | 11 | 35395205 | - | SLC1A2 | NNNTGTTGGTGCATGGCTTG | NGG | 2 | 0.6029 | Tier II |
7 | NC_000002.12 | 2 | 120761831 | + | GLI2 | NNNTCTTGGTGTATGGCTTG | NGG | 2 | 0.5 | Tier II |
8 | NC_000010.11 | 10 | 68744407 | + | CCAR1 | NNNAGTAGGTGGATGGCTTG | NGG | 2 | 0.4375 | Tier II |
9 | NC_000012.12 | 12 | 39645838 | - | REDIC1 | NNNAGTTGGAGGATGGCTTG | NGG | 2 | 0.4286 | Tier II |
10 | NC_000022.11 | 22 | 30827271 | + | OSBP2 | NNNAGGTGGTGTGTGGCTTG | NGG | 2 | 0.3727 | Tier II |
11 | NC_000011.10 | 11 | 114275638 | + | NNMT | NNNAGTGTGTGTATGGCTTG | NGG | 2 | 0.3676 | Tier II |
12 | NC_000016.10 | 16 | 23954834 | + | PRKCB | NNNAGTGGGTGGATGGCTTG | NGG | 2 | 0.2941 | Tier II |
13 | NC_000003.12 | 3 | 4527412 | - | ITPR1 | NNNAGGTGGTGGATGGCTTG | NGG | 2 | 0.2857 | Tier II |
14 | NC_000017.11 | 17 | 45037028 | + | DCAKD | NNNAGTTGGTGCCTGGCTTG | NGG | 2 | 0.1994 | Tier II |
15 | NC_000002.12 | 2 | 9914552 | - | TAF1B | NNNGGTTGGTGTCTGGCTTG | NGG | 2 | 0.1316 | Tier II |
16 | NC_000016.10 | 16 | 20949886 | + | DNAH3 | NNNAGTTGGTTTATGGCTTG | NAG | 2 | 0.0997 | Tier II |
17 | NC_000022.11 | 22 | 42004706 | + | WBP2NL | NNNAGTGGGTGTATTGCTTG | NGG | 2 | 0.084 | Tier II |
18 | NC_000001.11 | 1 | 27843735 | + | PPP1R8 | NNNAGTTGGTGGATTGCTTG | NGG | 2 | 0.0714 | Tier II |
19 | NC_000003.12 | 3 | 142307362 | + | XRN1 | NNNAGTTGGTGTACGGCTGG | NGG | 2 | 0.0714 | Tier II |
20 | NC_000001.11 | 1 | 107887738 | - | VAV3 | NNNTGTTGGTGTATGGGTTG | NGG | 2 | 0.0374 | Tier II |
21 | NC_000006.12 | 6 | 110276050 | - | METTL24 | NNNTGTTGGTGTATGGCTTG | NTG | 2 | 0.0248 | Tier II |
22 | NC_000001.11 | 1 | 72077572 | + | NEGR1 | NNNAGTTGGGGTATGGCTTG | NGC | 2 | 0.0111 | Tier II |
23 | NC_000016.10 | 16 | 11692113 | + | TXNDC11 | NNNAGTTGGTGAAGGGCTTG | NGG | 2 | 0.0 | Tier II |
24 | NC_000007.14 | 7 | 71940277 | + | CALN1 | NNNAGTTGGTGAATGCCTTG | NGG | 2 | 0.0 | Tier II |
25 | NC_000008.11 | 8 | 60789948 | + | CHD7 | NNNAGTTGGTGTATGCCTTG | NTG | 2 | 0.0 | Tier II |
26 | NC_000016.10 | 16 | 66144421 | - | LOC105371316 | NNNAGTAGGTGTATGACTTG | NGG | 2 | 0.875 | Tier III |
27 | NC_000003.12 | 3 | 90221082 | + | PROS2P | NNNAGTTGGTGAATAGCTTG | NGG | 2 | 0.7529 | Tier III |
28 | NC_000008.11 | 8 | 95389111 | + | CFAP418-AS1 | NNNAGATGGAGTATGGCTTG | NGG | 2 | 0.7429 | Tier III |
29 | NC_000020.11 | 20 | 31710756 | - | BCL2L1-AS1 | NNNACTTGGTGAATGGCTTG | NGG | 2 | 0.6286 | Tier III |
30 | NC_000002.12 | 2 | 97609886 | + | LOC105373499 | NNNAGTGGGTGGATGGCTTG | NGG | 2 | 0.2941 | Tier III |
31 | NC_000017.11 | 17 | 36892837 | - | LHX1-DT | NNNAGTTGGAGTTTGGCTTG | NGG | 2 | 0.2571 | Tier III |
32 | NC_000008.11 | 8 | 5130325 | + | LOC107986907 | NNNAGTTGGGGTATGGCTTG | NAG | 2 | 0.1296 | Tier III |
33 | NC_000002.12 | 2 | 24982718 | + | DNAJC27-AS1 | NNNGGTTGGTGTATTGCTTG | NGG | 2 | 0.0893 | Tier III |
34 | NC_000019.10 | 19 | 43689087 | - | LOC105372412 | NNNAGTGGGTGTATTGCTTG | NGG | 2 | 0.084 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 133914692 | + | Prelp | NNNAGCTGGTGGATGGCTTG | NGG | 2 | 0.4545 | Tier I |
2 | NC_000073.6 | 7 | 109727881 | + | Ascl3 | NNNAGTTGGTGTAAGGCATG | NGG | 2 | 0.4127 | Tier I |
3 | NC_000082.6 | 16 | 63793670 | - | Epha3 | NNNAGTTAATGTATGGCTTG | NGG | 2 | 0.6429 | Tier II |
4 | NC_000075.6 | 9 | 63687959 | - | Smad3 | NNNAATTGGTGTGTGGCTTG | NGG | 2 | 0.5652 | Tier II |
5 | NC_000067.6 | 1 | 91935504 | - | Hdac4 | NNNTGTTGGTGAATGGCTTG | NGG | 2 | 0.5091 | Tier II |
6 | NC_000078.6 | 12 | 53005561 | + | Akap6 | NNNAGTGGGTGTGTGGCTTG | NGG | 2 | 0.3836 | Tier II |
7 | NC_000076.6 | 10 | 44301201 | + | Atg5 | NNNAGTTGGTGTTTGGCTTT | NGG | 2 | 0.21 | Tier II |
8 | NC_000075.6 | 9 | 113759267 | - | Clasp2 | NNNTGTTGGTGTTTGGCTTG | NGG | 2 | 0.1909 | Tier II |
9 | NC_000071.6 | 5 | 36971741 | - | Wfs1 | NNNAGTGGGTGTATGGCTTG | NAG | 2 | 0.1525 | Tier II |
10 | NC_000077.6 | 11 | 116067965 | + | Unc13d | NNNAGTTGGTGTAAGGCTTG | NGT | 2 | 0.01 | Tier II |
11 | NC_000083.6 | 17 | 83598882 | - | Kcng3 | NNNACTTGGTGTAGGGCTTG | NGG | 2 | 0.0 | Tier II |
12 | NC_000072.6 | 6 | 106634982 | + | Cntn4 | NNNAGTTGGTGAAGGGCTTG | NGG | 2 | 0.0 | Tier II |
13 | NC_000068.7 | 2 | 165090250 | + | 1700025C18Rik | NNNAGTTGGTGGTTGGCTTG | NGG | 2 | 0.15 | Tier III |