Construct: sgRNA BRDN0001149057

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGAGTTGGTGTATGGCTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH1 (5681)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76738

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 67909565 - PSKH1 NNNAGTTGGTGTATGGCTTG NGG 0 1.0 Tier I
2 NC_000001.11 1 215766782 + USH2A NNNAGTTGGTGTATGGCTGG NGA 2 0.0174 Tier I
3 NC_000017.11 17 33369938 - ASIC2 NNNAGTTGGAGTATGGCTTA NGG 2 0.8036 Tier II
4 NC_000003.12 3 58069910 + FLNB NNNACTTGGAGTATGGCTTG NGG 2 0.6735 Tier II
5 NC_000020.11 20 31710756 - BCL2L1 NNNACTTGGTGAATGGCTTG NGG 2 0.6286 Tier II
6 NC_000011.10 11 35395205 - SLC1A2 NNNTGTTGGTGCATGGCTTG NGG 2 0.6029 Tier II
7 NC_000002.12 2 120761831 + GLI2 NNNTCTTGGTGTATGGCTTG NGG 2 0.5 Tier II
8 NC_000010.11 10 68744407 + CCAR1 NNNAGTAGGTGGATGGCTTG NGG 2 0.4375 Tier II
9 NC_000012.12 12 39645838 - REDIC1 NNNAGTTGGAGGATGGCTTG NGG 2 0.4286 Tier II
10 NC_000022.11 22 30827271 + OSBP2 NNNAGGTGGTGTGTGGCTTG NGG 2 0.3727 Tier II
11 NC_000011.10 11 114275638 + NNMT NNNAGTGTGTGTATGGCTTG NGG 2 0.3676 Tier II
12 NC_000016.10 16 23954834 + PRKCB NNNAGTGGGTGGATGGCTTG NGG 2 0.2941 Tier II
13 NC_000003.12 3 4527412 - ITPR1 NNNAGGTGGTGGATGGCTTG NGG 2 0.2857 Tier II
14 NC_000017.11 17 45037028 + DCAKD NNNAGTTGGTGCCTGGCTTG NGG 2 0.1994 Tier II
15 NC_000002.12 2 9914552 - TAF1B NNNGGTTGGTGTCTGGCTTG NGG 2 0.1316 Tier II
16 NC_000016.10 16 20949886 + DNAH3 NNNAGTTGGTTTATGGCTTG NAG 2 0.0997 Tier II
17 NC_000022.11 22 42004706 + WBP2NL NNNAGTGGGTGTATTGCTTG NGG 2 0.084 Tier II
18 NC_000001.11 1 27843735 + PPP1R8 NNNAGTTGGTGGATTGCTTG NGG 2 0.0714 Tier II
19 NC_000003.12 3 142307362 + XRN1 NNNAGTTGGTGTACGGCTGG NGG 2 0.0714 Tier II
20 NC_000001.11 1 107887738 - VAV3 NNNTGTTGGTGTATGGGTTG NGG 2 0.0374 Tier II
21 NC_000006.12 6 110276050 - METTL24 NNNTGTTGGTGTATGGCTTG NTG 2 0.0248 Tier II
22 NC_000001.11 1 72077572 + NEGR1 NNNAGTTGGGGTATGGCTTG NGC 2 0.0111 Tier II
23 NC_000016.10 16 11692113 + TXNDC11 NNNAGTTGGTGAAGGGCTTG NGG 2 0.0 Tier II
24 NC_000007.14 7 71940277 + CALN1 NNNAGTTGGTGAATGCCTTG NGG 2 0.0 Tier II
25 NC_000008.11 8 60789948 + CHD7 NNNAGTTGGTGTATGCCTTG NTG 2 0.0 Tier II
26 NC_000016.10 16 66144421 - LOC105371316 NNNAGTAGGTGTATGACTTG NGG 2 0.875 Tier III
27 NC_000003.12 3 90221082 + PROS2P NNNAGTTGGTGAATAGCTTG NGG 2 0.7529 Tier III
28 NC_000008.11 8 95389111 + CFAP418-AS1 NNNAGATGGAGTATGGCTTG NGG 2 0.7429 Tier III
29 NC_000020.11 20 31710756 - BCL2L1-AS1 NNNACTTGGTGAATGGCTTG NGG 2 0.6286 Tier III
30 NC_000002.12 2 97609886 + LOC105373499 NNNAGTGGGTGGATGGCTTG NGG 2 0.2941 Tier III
31 NC_000017.11 17 36892837 - LHX1-DT NNNAGTTGGAGTTTGGCTTG NGG 2 0.2571 Tier III
32 NC_000008.11 8 5130325 + LOC107986907 NNNAGTTGGGGTATGGCTTG NAG 2 0.1296 Tier III
33 NC_000002.12 2 24982718 + DNAJC27-AS1 NNNGGTTGGTGTATTGCTTG NGG 2 0.0893 Tier III
34 NC_000019.10 19 43689087 - LOC105372412 NNNAGTGGGTGTATTGCTTG NGG 2 0.084 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 133914692 + Prelp NNNAGCTGGTGGATGGCTTG NGG 2 0.4545 Tier I
2 NC_000073.6 7 109727881 + Ascl3 NNNAGTTGGTGTAAGGCATG NGG 2 0.4127 Tier I
3 NC_000082.6 16 63793670 - Epha3 NNNAGTTAATGTATGGCTTG NGG 2 0.6429 Tier II
4 NC_000075.6 9 63687959 - Smad3 NNNAATTGGTGTGTGGCTTG NGG 2 0.5652 Tier II
5 NC_000067.6 1 91935504 - Hdac4 NNNTGTTGGTGAATGGCTTG NGG 2 0.5091 Tier II
6 NC_000078.6 12 53005561 + Akap6 NNNAGTGGGTGTGTGGCTTG NGG 2 0.3836 Tier II
7 NC_000076.6 10 44301201 + Atg5 NNNAGTTGGTGTTTGGCTTT NGG 2 0.21 Tier II
8 NC_000075.6 9 113759267 - Clasp2 NNNTGTTGGTGTTTGGCTTG NGG 2 0.1909 Tier II
9 NC_000071.6 5 36971741 - Wfs1 NNNAGTGGGTGTATGGCTTG NAG 2 0.1525 Tier II
10 NC_000077.6 11 116067965 + Unc13d NNNAGTTGGTGTAAGGCTTG NGT 2 0.01 Tier II
11 NC_000083.6 17 83598882 - Kcng3 NNNACTTGGTGTAGGGCTTG NGG 2 0.0 Tier II
12 NC_000072.6 6 106634982 + Cntn4 NNNAGTTGGTGAAGGGCTTG NGG 2 0.0 Tier II
13 NC_000068.7 2 165090250 + 1700025C18Rik NNNAGTTGGTGGTTGGCTTG NGG 2 0.15 Tier III
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Other clones with same target sequence:

(none)