Construct: sgRNA BRDN0001149064
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGTACATCACATGTGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME9 (347736)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76835
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
138304996 |
- |
NME9 |
NNNGTACATCACATGTGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
183078926 |
- |
DUSP19 |
NNNGTACATTACATGAGCAG |
NGG |
2 |
0.8556 |
Tier I |
| 3 |
NC_000001.11 |
1 |
20049634 |
- |
PLA2G5 |
NNNGCACATAACATGTGCAG |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000003.12 |
3 |
131782406 |
- |
CPNE4 |
NNNGTGCATCACATGTGCAA |
NGG |
2 |
0.6696 |
Tier II |
| 5 |
NC_000005.10 |
5 |
147047077 |
+ |
PPP2R2B |
NNNGGACATCACATGTGAAG |
NGG |
2 |
0.3446 |
Tier II |
| 6 |
NC_000010.11 |
10 |
5204838 |
- |
AKR1C4 |
NNNGTACACCACATGTGCAG |
NAG |
2 |
0.2393 |
Tier II |
| 7 |
NC_000010.11 |
10 |
5097903 |
- |
AKR1C3 |
NNNGTGCATCACATGTGCAG |
NAG |
2 |
0.1852 |
Tier II |
| 8 |
NC_000011.10 |
11 |
60519244 |
+ |
MS4A13 |
NNNGTACATCAGATGGGCAG |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000002.12 |
2 |
1171861 |
+ |
SNTG2 |
NNNGCACATCACATTTGCAG |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000006.12 |
6 |
38141643 |
+ |
ZFAND3 |
NNNGTACATCATATGTTCAG |
NGG |
2 |
0.1346 |
Tier II |
| 11 |
NC_000010.11 |
10 |
51938660 |
- |
PRKG1 |
NNNGGACATCACATGTGCAG |
NTG |
2 |
0.0249 |
Tier II |
| 12 |
NC_000001.11 |
1 |
197776940 |
+ |
DENND1B |
NNNGTACATCACATGAGCAG |
NGT |
2 |
0.0147 |
Tier II |
| 13 |
NC_000011.10 |
11 |
128856509 |
- |
KCNJ1 |
NNNGTACATCACATGTGGAG |
NTG |
2 |
0.0052 |
Tier II |
| 14 |
NC_000002.12 |
2 |
102370956 |
+ |
IL18R1 |
NNNATACATCACAGGTGCAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000003.12 |
3 |
164524718 |
- |
LOC105374191 |
NNNGTACATTCCATGTGCAG |
NGG |
2 |
0.3765 |
Tier III |
| 16 |
NC_000017.11 |
17 |
27346577 |
+ |
LOC124903957 |
NNNGTCCATCATATGTGCAG |
NGG |
2 |
0.2448 |
Tier III |
| 17 |
NC_000017.11 |
17 |
27346577 |
+ |
LOC124903959 |
NNNGTCCATCATATGTGCAG |
NGG |
2 |
0.2448 |
Tier III |
| 18 |
NC_000016.10 |
16 |
79757343 |
+ |
MAFTRR |
NNNTTAGATCACATGTGCAG |
NGG |
2 |
0.1711 |
Tier III |
| 19 |
NC_000006.12 |
6 |
38141643 |
+ |
LOC124901314 |
NNNGTACATCATATGTTCAG |
NGG |
2 |
0.1346 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
99469633 |
+ |
Nme9 |
NNNGTACATCACATGTGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
133982920 |
+ |
Adam12 |
NNNGCACACCACATGTGCAG |
NGG |
2 |
0.9231 |
Tier II |
| 3 |
NC_000081.6 |
15 |
84812380 |
+ |
Phf21b |
NNNGTTCATTACATGTGCAG |
NGG |
2 |
0.6723 |
Tier II |
| 4 |
NC_000077.6 |
11 |
103139282 |
+ |
Hexim2 |
NNNGTGCACCACATGTGCAG |
NGG |
2 |
0.6593 |
Tier II |
| 5 |
NC_000080.6 |
14 |
75477052 |
+ |
Siah3 |
NNNGCACATCACAAGTGCAG |
NGG |
2 |
0.619 |
Tier II |
| 6 |
NC_000072.6 |
6 |
95491323 |
- |
Suclg2 |
NNNGTACAACACATGTGTAG |
NGG |
2 |
0.5969 |
Tier II |
| 7 |
NC_000072.6 |
6 |
29746522 |
+ |
Smo |
NNNGTGCATCACATGTGCAT |
NGG |
2 |
0.5 |
Tier II |
| 8 |
NC_000074.6 |
8 |
3543226 |
+ |
Pnpla6 |
NNNGCAGATCACATGTGCAG |
NGG |
2 |
0.4706 |
Tier II |
| 9 |
NC_000077.6 |
11 |
75551392 |
+ |
Slc43a2 |
NNNGCACATGACATGTGCAG |
NGG |
2 |
0.3889 |
Tier II |
| 10 |
NC_000070.6 |
4 |
76502730 |
- |
Ptprd |
NNNGTACATTCCATGTGCAG |
NGG |
2 |
0.3765 |
Tier II |
| 11 |
NC_000075.6 |
9 |
50537063 |
+ |
Bco2 |
NNNGTTCCTCACATGTGCAG |
NGG |
2 |
0.3061 |
Tier II |
| 12 |
NC_000085.6 |
19 |
55792125 |
+ |
Tcf7l2 |
NNNGTACATCACAACTGCAG |
NGG |
2 |
0.1688 |
Tier II |
| 13 |
NC_000067.6 |
1 |
150364230 |
- |
Odr4 |
NNNGTACATCACATGTTCTG |
NGG |
2 |
0.1346 |
Tier II |
| 14 |
NC_000074.6 |
8 |
109380710 |
- |
Pmfbp1 |
NNNATACATCACATTTGCAG |
NGG |
2 |
0.1286 |
Tier II |
| 15 |
NC_000084.6 |
18 |
78296045 |
- |
Slc14a2 |
NNNATACATCACATTTGCAG |
NGG |
2 |
0.1286 |
Tier II |
| 16 |
NC_000073.6 |
7 |
132952756 |
+ |
Zranb1 |
NNNGTAAATCACATTTGCAG |
NGG |
2 |
0.1071 |
Tier II |
| 17 |
NC_000083.6 |
17 |
80040915 |
- |
Hnrnpll |
NNNGTACATCACATGTGCAG |
NTG |
1 |
0.039 |
Tier II |
| 18 |
NC_000075.6 |
9 |
67911122 |
+ |
Vps13c |
NNNATACATCACATGTGCAG |
NGC |
2 |
0.02 |
Tier II |
| 19 |
NC_000073.6 |
7 |
113310825 |
- |
Arntl |
NNNGTATATCACATGTGCAG |
NGC |
2 |
0.0181 |
Tier II |
| 20 |
NC_000072.6 |
6 |
65130277 |
+ |
Hpgds |
NNNGTACATCACATGTGTAG |
NGC |
2 |
0.0143 |
Tier II |
| 21 |
NC_000078.6 |
12 |
69262435 |
+ |
Klhdc1 |
NNNGTGCATCACATGTGCAG |
NGT |
2 |
0.0115 |
Tier II |
| 22 |
NC_000080.6 |
14 |
9450142 |
+ |
Gm41097 |
NNNTTACATCACATGTGCTG |
NGG |
2 |
0.1958 |
Tier III |
| 23 |
NC_000077.6 |
11 |
20026100 |
+ |
Gm39603 |
NNNGGACATCACATGTGGAG |
NGG |
2 |
0.0853 |
Tier III |
Other clones with same target sequence:
(none)
