Construct: sgRNA BRDN0001149068
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTATTCATAAGGGATGAGGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAOK3 (51347)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76734
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
118189919 |
- |
TAOK3 |
NNNTTCATAAGGGATGAGGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
7041893 |
- |
SLC16A11 |
NNNTTCCTAAGGGATGAGAC |
NGG |
2 |
0.3125 |
Tier I |
| 3 |
NC_000006.12 |
6 |
52025362 |
- |
PKHD1 |
NNNTTCATAAGGGAAGAGGC |
NAG |
2 |
0.1501 |
Tier I |
| 4 |
NC_000002.12 |
2 |
74012579 |
+ |
TET3 |
NNNTTCACAAGGGATGAAGC |
NGG |
2 |
0.6923 |
Tier II |
| 5 |
NC_000007.14 |
7 |
88785646 |
- |
ZNF804B |
NNNGTCATAAGAGATGAGGC |
NGG |
2 |
0.5833 |
Tier II |
| 6 |
NC_000010.11 |
10 |
13506465 |
+ |
BEND7 |
NNNTTCATAGGAGATGAGGC |
NGG |
2 |
0.5185 |
Tier II |
| 7 |
NC_000002.12 |
2 |
69186079 |
- |
ANTXR1 |
NNNTTAATAAGGGATGAGCC |
NGG |
2 |
0.4163 |
Tier II |
| 8 |
NC_000021.9 |
21 |
44927353 |
+ |
ITGB2 |
NNNTTCATAAGGGATGAGAA |
NGG |
2 |
0.3571 |
Tier II |
| 9 |
NC_000015.10 |
15 |
101428444 |
- |
PCSK6 |
NNNTGCATAGGGGATGAGGC |
NGG |
2 |
0.3556 |
Tier II |
| 10 |
NC_000006.12 |
6 |
42114336 |
- |
C6orf132 |
NNNTTCTGAAGGGATGAGGC |
NGG |
2 |
0.3208 |
Tier II |
| 11 |
NC_000020.11 |
20 |
6038889 |
- |
CRLS1 |
NNNTTCCTAAGGGATGAAGC |
NGG |
2 |
0.3029 |
Tier II |
| 12 |
NC_000002.12 |
2 |
50167216 |
- |
NRXN1 |
NNNTGCATAAGGGGTGAGGC |
NGG |
2 |
0.2987 |
Tier II |
| 13 |
NC_000005.10 |
5 |
66993754 |
+ |
MAST4 |
NNNTTGTTAAGGGATGAGGC |
NGG |
2 |
0.2188 |
Tier II |
| 14 |
NC_000002.12 |
2 |
189069076 |
+ |
COL5A2 |
NNNTTCTTAAGGGATGACGC |
NGG |
2 |
0.2083 |
Tier II |
| 15 |
NC_000003.12 |
3 |
69785205 |
+ |
MITF |
NNNTTCATGAGGGCTGAGGC |
NGG |
2 |
0.1429 |
Tier II |
| 16 |
NC_000004.12 |
4 |
42119034 |
- |
BEND4 |
NNNTTCAGAAGGGATGGGGC |
NGG |
2 |
0.1294 |
Tier II |
| 17 |
NC_000018.10 |
18 |
11760678 |
+ |
GNAL |
NNNTTCATAGGGGCTGAGGC |
NGG |
2 |
0.119 |
Tier II |
| 18 |
NC_000023.11 |
X |
15549470 |
+ |
BMX |
NNNTTCATAAGGGTTGGGGC |
NGG |
2 |
0.0941 |
Tier II |
| 19 |
NC_000023.11 |
X |
15549470 |
+ |
ACE2 |
NNNTTCATAAGGGTTGGGGC |
NGG |
2 |
0.0941 |
Tier II |
| 20 |
NC_000005.10 |
5 |
36896118 |
+ |
NIPBL |
NNNTTCATAAGGTATGAGGT |
NGG |
2 |
0.09 |
Tier II |
| 21 |
NC_000001.11 |
1 |
1758722 |
- |
NADK |
NNNTTCAGAAGGGATTAGGC |
NGG |
2 |
0.0 |
Tier II |
| 22 |
NC_000002.12 |
2 |
224642513 |
+ |
LOC105373909 |
NNNGTCATCAGGGATGAGGC |
NGG |
2 |
0.3571 |
Tier III |
| 23 |
NC_000021.9 |
21 |
44927353 |
+ |
ITGB2-AS1 |
NNNTTCATAAGGGATGAGAA |
NGG |
2 |
0.3571 |
Tier III |
| 24 |
NC_000023.11 |
X |
16448098 |
- |
LOC124905249 |
NNNTTCATAAGGCAAGAGGC |
NGG |
2 |
0.2438 |
Tier III |
| 25 |
NC_000004.12 |
4 |
183495006 |
- |
ING2-DT |
NNNTTCATAAAGGCTGAGGC |
NGG |
2 |
0.2143 |
Tier III |
| 26 |
NC_000005.10 |
5 |
57404075 |
+ |
RMEL3 |
NNNTTCATAAGGGATGGGGC |
NGG |
1 |
0.1765 |
Tier III |
| 27 |
NC_000011.10 |
11 |
83085427 |
+ |
LINC02951 |
NNNTTCATCAGGGATGAGGT |
NGG |
2 |
0.1714 |
Tier III |
| 28 |
NC_000001.11 |
1 |
29620345 |
- |
LOC107984934 |
NNNCTCATAAGGGATGAGGC |
NGT |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
117241119 |
+ |
Taok3 |
NNNTTCGTAAGGGATGAGGC |
NGG |
1 |
0.7059 |
Tier I |
| 2 |
NC_000068.7 |
2 |
10233135 |
+ |
Itih5 |
NNNTTCATGAAGGATGAGGC |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000086.7 |
X |
12083894 |
- |
Bcor |
NNNTTCATGGGGGATGAGGC |
NGG |
2 |
0.3704 |
Tier II |
| 4 |
NC_000086.7 |
X |
83315161 |
+ |
Dmd |
NNNTTCATAAGGGTTGAGGA |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000068.7 |
2 |
146195530 |
+ |
Cfap61 |
NNNTTCAAAAGGGCTGAGGC |
NGG |
2 |
0.1714 |
Tier II |
| 6 |
NC_000080.6 |
14 |
60613333 |
- |
Spata13 |
NNNTTCAGAAGGGCTGAGGC |
NGG |
2 |
0.1571 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48234841 |
+ |
Sorcs3 |
NNNTTCAAAAGGGATGTGGC |
NGG |
2 |
0.1067 |
Tier II |
| 8 |
NC_000069.6 |
3 |
30794040 |
- |
Sec62 |
NNNTTCATAAGGGATGAGGG |
NGG |
1 |
0.0588 |
Tier II |
| 9 |
NC_000075.6 |
9 |
50880156 |
- |
Ppp2r1b |
NNNTTCAAAAGGGAGGAGGC |
NGG |
2 |
0.04 |
Tier II |
| 10 |
NC_000081.6 |
15 |
10205974 |
- |
Prlr |
NNNTTCATATGGGATGAGGC |
NGT |
2 |
0.0142 |
Tier II |
| 11 |
NC_000069.6 |
3 |
142253487 |
- |
Pdlim5 |
NNNTTCATAAGGGCTGAGGG |
NGG |
2 |
0.0126 |
Tier II |
| 12 |
NC_000079.6 |
13 |
77520878 |
+ |
2210408I21Rik |
NNNTTCATAAGGGATTAGGC |
NAG |
2 |
0.0 |
Tier II |
| 13 |
NC_000076.6 |
10 |
44618006 |
- |
Gm35154 |
NNNTTCAGAAAGGATGAGGC |
NGG |
2 |
0.7333 |
Tier III |
| 14 |
NC_000078.6 |
12 |
52450189 |
- |
Gm35071 |
NNNTTCATAAGGCATGATGC |
NGG |
2 |
0.2807 |
Tier III |
| 15 |
NC_000071.6 |
5 |
123007880 |
+ |
Gm2479 |
NNNGTCATAAGGGAGGAGGC |
NGG |
2 |
0.0313 |
Tier III |
| 16 |
NC_000073.6 |
7 |
93005629 |
+ |
Gm31663 |
NNNTTGATAAGGGAGGAGGC |
NGG |
2 |
0.025 |
Tier III |
Other clones with same target sequence:
(none)
