Construct: sgRNA BRDN0001149083

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCAGTGAAAACATTAAAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRK (2444)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76241

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 115967556 - FRK NNNGTGAAAACATTAAAACC NGG 0 1.0 Tier I
2 NC_000009.12 9 97328022 - CCDC180 NNNGTGAAAACATTCATACC NGG 2 0.0364 Tier I
3 NC_000012.12 12 2803942 + FKBP4 NNNGTGGAAACAATAAAACC NGG 2 0.4887 Tier II
4 NC_000004.12 4 153204663 - TRIM2 NNNGGGAAAAAATTAAAACC NGG 2 0.48 Tier II
5 NC_000003.12 3 60994794 + FHIT NNNGAGAAAACACTAAAACC NGG 2 0.3947 Tier II
6 NC_000015.10 15 99359716 + LRRC28 NNNGAGAAAACATTGAAACC NGG 2 0.325 Tier II
7 NC_000017.11 17 630592 + VPS53 NNNGTGAAGACATTAAAATC NGG 2 0.3077 Tier II
8 NC_000010.11 10 67219043 - CTNNA3 NNNGTGACAACATAAAAACC NGG 2 0.2653 Tier II
9 NC_000006.12 6 131012845 + EPB41L2 NNNGTAAAAACATTAAAACC NAG 2 0.2593 Tier II
10 NC_000011.10 11 26467073 + ANO3 NNNGTGAAAACATTAAATCA NGG 2 0.25 Tier II
11 NC_000020.11 20 8271455 + PLCB1 NNNATGAAAACCTTAAAACC NGG 2 0.2368 Tier II
12 NC_000005.10 5 45265277 - HCN1 NNNGAGAAAACATTAAAATC NGG 2 0.2308 Tier II
13 NC_000010.11 10 98143678 + R3HCC1L NNNGAGAAAACATTAAAATC NGG 2 0.2308 Tier II
14 NC_000013.11 13 94105633 + GPC6 NNNGTGAAAACATTAAATTC NGG 2 0.2308 Tier II
15 NC_000001.11 1 45442348 - TESK2 NNNGTGAAAACTATAAAACC NGG 2 0.2308 Tier II
16 NC_000006.12 6 119025560 - FAM184A NNNGTGAAATCATTAAAACC NAG 2 0.2288 Tier II
17 NC_000022.11 22 43541501 + EFCAB6 NNNGTGAAAACACCAAAACC NGG 2 0.2256 Tier II
18 NC_000015.10 15 40245404 + PAK6 NNNGTTAAAACTTTAAAACC NGG 2 0.2222 Tier II
19 NC_000015.10 15 40245404 + BUB1B-PAK6 NNNGTTAAAACTTTAAAACC NGG 2 0.2222 Tier II
20 NC_000012.12 12 116725530 + SPRING1 NNNGTGATAACATTAAAAAC NGG 2 0.1837 Tier II
21 NC_000008.11 8 1212090 + DLGAP2 NNNATGAAAACATTTAAACC NGG 2 0.18 Tier II
22 NC_000002.12 2 143522492 + ARHGAP15 NNNGTGAAAATATTAAAACA NGG 2 0.1538 Tier II
23 NC_000001.11 1 108955901 - CLCC1 NNNGTGAAAGCCTTAAAACC NGG 2 0.1462 Tier II
24 NC_000004.12 4 182049895 + TENM3 NNNCTGAAAACATTAAAACC NAG 2 0.1373 Tier II
25 NC_000013.11 13 98967844 - DOCK9 NNNGTGAAGACATTTAAACC NGG 2 0.1333 Tier II
26 NC_000006.12 6 44831783 - SUPT3H NNNCTGAAAAGATTAAAACC NGG 2 0.1324 Tier II
27 NC_000010.11 10 25549450 + GPR158 NNNGTGAAAACAGTAAAAAC NGG 2 0.1118 Tier II
28 NC_000012.12 12 14682709 + GUCY2C NNNGTGAAAACATTAACTCC NGG 2 0.0882 Tier II
29 NC_000011.10 11 24591630 + LUZP2 NNNGTGAAAACTTTAAAACC NAG 2 0.0864 Tier II
30 NC_000010.11 10 34461002 + PARD3 NNNGTGAAAATATTAAAACC NAG 2 0.0798 Tier II
31 NC_000006.12 6 36307188 - PNPLA1 NNNGTGCAAACATTAAAAGC NGG 2 0.0547 Tier II
32 NC_000005.10 5 134550163 - JADE2 NNNGTGAAAAAATTAAAACC NGA 2 0.0521 Tier II
33 NC_000011.10 11 55891810 + TRIM51 NNNGTGAAAACATTAAAACC NTG 1 0.039 Tier II
34 NC_000002.12 2 190429028 - MFSD6 NNNGTGAAAGCATTAAAACC NGA 2 0.0386 Tier II
35 NC_000002.12 2 190429028 - NEMP2 NNNGTGAAAGCATTAAAACC NGA 2 0.0386 Tier II
36 NC_000002.12 2 169018505 + ABCB11 NNNGAGAAAACATTAAAACC NTG 2 0.0195 Tier II
37 NC_000023.11 X 37889521 - SYTL5 NNNGTAAAAACATTAAAACC NGT 2 0.0161 Tier II
38 NC_000012.12 12 3387704 + PRMT8 NNNTTGAAAACATTAAAACC NGC 2 0.0081 Tier II
39 NC_000010.11 10 22599215 - PIP4K2A NNNGTGAAAACATTAAAAAC NGT 2 0.0069 Tier II
40 NC_000020.11 20 33851702 - CHMP4B NNNGTGAAAACATGAAAACC NTG 2 0.0 Tier II
41 NC_000017.11 17 67138356 + HELZ NNNGTGAAAACATGAAAATC NGG 2 0.0 Tier II
42 NC_000003.12 3 15021575 + NR2C2 NNNGTGAAAACATGAAGACC NGG 2 0.0 Tier II
43 NC_000003.12 3 195128385 - XXYLT1 NNNGTGAAAACATGAATACC NGG 2 0.0 Tier II
44 NC_000002.12 2 48561806 - STON1 NNNGTGAAAACCTGAAAACC NGG 2 0.0 Tier II
45 NC_000002.12 2 48561806 - STON1-GTF2A1L NNNGTGAAAACCTGAAAACC NGG 2 0.0 Tier II
46 NC_000012.12 12 2803942 + ITFG2-AS1 NNNGTGGAAACAATAAAACC NGG 2 0.4887 Tier III
47 NC_000007.14 7 24476546 + LOC124901601 NNNGTCAAAACAATAAAACC NGG 2 0.472 Tier III
48 NC_000020.11 20 48056950 + LOC105372639 NNNAAGAAAACATTAAAACC NGG 2 0.45 Tier III
49 NC_000001.11 1 189178359 + LOC105371657 NNNGTGAAAACATAAAAACA NGG 2 0.3095 Tier III
50 NC_000005.10 5 118510868 + LINC02208 NNNGTGAAGACATTAAAATC NGG 2 0.3077 Tier III
51 NC_000007.14 7 136263722 - LOC105375523 NNNGTTACAACATTAAAACC NGG 2 0.2857 Tier III
52 NC_000006.12 6 119025560 - LOC124901389 NNNGTGAAATCATTAAAACC NAG 2 0.2288 Tier III
53 NC_000011.10 11 129778333 + LOC124902793 NNNCTGAAAACATTAAAAAC NGG 2 0.2269 Tier III
54 NC_000011.10 11 116349014 + LOC107987166 NNNGTGAAAACCTTGAAACC NGG 2 0.1711 Tier III
55 NC_000018.10 18 55666965 + LOC105372130 NNNTTGAAAACATTAAAATC NGG 2 0.1678 Tier III
56 NC_000006.12 6 44831783 - LOC101929770 NNNCTGAAAAGATTAAAACC NGG 2 0.1324 Tier III
57 NC_000012.12 12 14682709 + GUCY2C-AS1 NNNGTGAAAACATTAACTCC NGG 2 0.0882 Tier III
58 NC_000009.12 9 97328022 - SUGT1P4-STRA6LP-CCDC180 NNNGTGAAAACATTCATACC NGG 2 0.0364 Tier III
59 NC_000011.10 11 89863071 + TRIM51BP NNNGTGAAAACACTAAAACC NTG 2 0.0308 Tier III
60 NC_000011.10 11 89981440 - TRIM51EP NNNGTGAAAACACTAAAACC NTG 2 0.0308 Tier III
61 NC_000013.11 13 53144421 - LINC01065 NNNGAGAAAACATTAAAACC NTG 2 0.0195 Tier III
62 NC_000003.12 3 176371563 + LOC124909460 NNNGTAAAAACATTAAAACC NGT 2 0.0161 Tier III
63 NC_000001.11 1 78327667 + MGC27382 NNNGTGAAAACATGGAAACC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 34586330 + Frk NNNGTAAAAACGTTAAAACC NGG 2 0.7222 Tier I
2 NC_000078.6 12 81729814 - Map3k9 NNNGTGAAAGCAGTAAAACC NGG 2 0.1449 Tier I
3 NC_000080.6 14 67706598 + Cdca2 NNNGTAAAAAAATTAAAACC NGG 2 0.75 Tier II
4 NC_000084.6 18 50065928 - Tnfaip8 NNNGTGAAAAAATAAAAACC NGG 2 0.4643 Tier II
5 NC_000076.6 10 3753747 - Plekhg1 NNNGTGTAATCATTAAAACC NGG 2 0.386 Tier II
6 NC_000068.7 2 93320523 - Tspan18 NNNGAGAAAAAATTAAAACC NGG 2 0.375 Tier II
7 NC_000077.6 11 104999530 - Gm11639 NNNGAGAAAACATTGAAACC NGG 2 0.325 Tier II
8 NC_000081.6 15 11266707 - Adamts12 NNNGTGAAAACACTAAAACC NAG 2 0.2047 Tier II
9 NC_000081.6 15 54451640 + Colec10 NNNGTCAAAACATTAAAACT NGG 2 0.2045 Tier II
10 NC_000077.6 11 87278689 - Ppm1e NNNGTGTAAACATTAAAAAC NGG 2 0.1875 Tier II
11 NC_000086.7 X 138377366 - Il1rapl2 NNNGTGAAAATATTAAAACA NGG 2 0.1538 Tier II
12 NC_000075.6 9 123816807 + Fyco1 NNNGTGAAAACATTAAATCC NAG 2 0.1296 Tier II
13 NC_000083.6 17 73156090 + Lclat1 NNNGTGACAACATTAAAACT NGG 2 0.1286 Tier II
14 NC_000067.6 1 86217627 - Armc9 NNNGTGAAGACATTAGAACC NGG 2 0.1282 Tier II
15 NC_000086.7 X 36774824 + Slc25a43 NNNGAGAAAAGATTAAAACC NGG 2 0.125 Tier II
16 NC_000069.6 3 119747719 + Ptbp2 NNNGTGAAAACATTAAAATC NAG 2 0.1197 Tier II
17 NC_000067.6 1 140253669 + Kcnt2 NNNGTGAAGACATTAAGACC NGG 2 0.1176 Tier II
18 NC_000068.7 2 119385323 + Ino80 NNNGGGAAAACATTAACACC NGG 2 0.1129 Tier II
19 NC_000073.6 7 91956566 + Dlg2 NNNTTGAAAACATTAAAACC NAG 2 0.0943 Tier II
20 NC_000070.6 4 65788180 + Astn2 NNNGTGAAAACAGTAAAACT NGG 2 0.0783 Tier II
21 NC_000079.6 13 102775769 - Mast4 NNNGTGAAAACATTAATAAC NGG 2 0.0571 Tier II
22 NC_000076.6 10 14071881 - Hivep2 NNNATGAAAACATTAAAACG NGG 2 0.0529 Tier II
23 NC_000081.6 15 92173057 - Cntn1 NNNGTGAAATCATTAAAACG NGG 2 0.0519 Tier II
24 NC_000076.6 10 115399970 - Zfc3h1 NNNGTGAAAACATTAAAATC NTG 2 0.018 Tier II
25 NC_000078.6 12 72287363 + Rtn1 NNNGTGAAAACATTAAATCC NGC 2 0.0111 Tier II
26 NC_000071.6 5 29767568 - Dnajb6 NNNGTGAAAACATTAGAACC NGT 2 0.0031 Tier II
27 NC_000069.6 3 59024090 - Med12l NNNGTGAAAACATTAATACC NGC 2 0.003 Tier II
28 NC_000082.6 16 74442718 - Robo2 NNNGTGAAAACATGAAAACC NAG 2 0.0 Tier II
29 NC_000076.6 10 3753747 - Gm30164 NNNGTGTAATCATTAAAACC NGG 2 0.386 Tier III
30 NC_000068.7 2 93320523 - Gm39881 NNNGAGAAAAAATTAAAACC NGG 2 0.375 Tier III
31 NC_000082.6 16 78046245 + Gm31431 NNNGAGATAACATTAAAACC NGG 2 0.2143 Tier III
32 NC_000073.6 7 91956566 + Gm32353 NNNTTGAAAACATTAAAACC NAG 2 0.0943 Tier III
33 NC_000081.6 15 92173057 - Gm36901 NNNGTGAAATCATTAAAACG NGG 2 0.0519 Tier III
34 NC_000086.7 X 105169749 - Gm33605 NNNGTGAACACATTAAAACC NTG 2 0.0223 Tier III
35 NC_000084.6 18 45718298 - A330093E20Rik NNNGTGAAAACATTAGAACC NGA 2 0.0134 Tier III
36 NC_000082.6 16 38829513 - Gm15802 NNNGTGAAAACCTTAAAACC NGC 2 0.0058 Tier III
37 NC_000068.7 2 147102170 + Gm34889 NNNTTGAAAACATTACAACC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)